Taurolithocholic acid 3-sulfate disodium
Based on 1 Customer Validation
Taurolithocholic acid 3-sulfate disodium is a GPR39 agonist with EC50s of 71.6 and 69.4 (absence of Zn2+) and 9 and 9.6 μM (presence of Zn2+) in M39-20 and hGPR39-2 cells, respectively. Taurolithocholic acid 3-sulfate disodium stimulates GPR39 receptors to initiate intracellular calcium signaling, independent of Zn2+ binding sites H17 and H19. Taurolithocholic acid 3-sulfate disodium can be used for the research of gallbladder disease.
For research use only. We do not sell to patients.
- Purity : 99.9%
- CAS No.: 64936-83-0
- Formula: C26H43NNa2O8S2
- Molecular Weight:607.73
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-113238A
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Tauro-α-muricholic acid sodium -
HY-114360A
Taurohyodeoxycholic acid sodium -
HY-W587430
Glycolithocholic acid 3-sulfate disodium -
HY-N0545
Taurocholic acid sodium -
HY-N0324S
Cholic acid-d4 -
HY-76847A
Chenodeoxycholic acid sodium -
HY-N2027S
Taurochenodeoxycholic acid-d4 sodium -
HY-N0545R
Taurocholic acid sodium (Standard) -
HY-B1899BS
Taurodeoxycholic acid-d4-1 sodium
Biological Activity
Description
IC50 & Target
EC50s: GPR39; 71.6 and 69.4 (absence of Zn2+) and 9 and 9.6 μM (presence of Zn2+) in M39-20 and hGPR39-2 cells, respectively[1]
In Vitro
Taurolithocholic acid 3-sulfate disodium is a GPR39 agonist with EC50s of 71.6 and 69.4 (absence of Zn2+) and 9 and 9.6 μM (presence of Zn2+) in M39-20 and hGPR39-2 cells, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 64936-83-0
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Appearance Solid
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Molecular Weight 607.73
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Formula C26H43NNa2O8S2
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Color White to off-white
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SMILES
C[C@H](CCC(NCCS(=O)(O[Na])=O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])CC[C@]4([H])C[C@H](OS(=O)(O[Na])=O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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Synonyms
3-Sulfotaurolithocholic acid disodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (41.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.06 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.06 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6455 mL | 8.2273 mL | 16.4547 mL | 41.1367 mL |
| 5 mM | 0.3291 mL | 1.6455 mL | 3.2909 mL | 8.2273 mL | |
| 10 mM | 0.1645 mL | 0.8227 mL | 1.6455 mL | 4.1137 mL | |
| 15 mM | 0.1097 mL | 0.5485 mL | 1.0970 mL | 2.7424 mL | |
| 20 mM | 0.0823 mL | 0.4114 mL | 0.8227 mL | 2.0568 mL | |
| 25 mM | 0.0658 mL | 0.3291 mL | 0.6582 mL | 1.6455 mL | |
| 30 mM | 0.0548 mL | 0.2742 mL | 0.5485 mL | 1.3712 mL | |
| 40 mM | 0.0411 mL | 0.2057 mL | 0.4114 mL | 1.0284 mL |
Taurolithocholic acid 3-sulfate disodium Related Classifications
HY-137255 Related Classifications
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