Lithocholic acid 3-sulfate disodium
Based on 1 Customer Validation
Lithocholic acid 3-sulfate disodium is a GPR39 agonist, with EC50 values of 41 μM and 42.4 μM in M39-20 and hGPR39-2 cells, respectively, in the absence of Zn2+, and 0.88 μM and 0.97 μM in the presence of Zn2+. Lithocholic acid 3-sulfate disodium acts as a RORγt ligand. Lithocholic acid 3-sulfate disodium stimulates the GPR39 receptor to initiate intracellular calcium signaling, independent of the Zn2+-binding sites H17 and H19. LCA-3-S selectively inhibits Th17 cell differentiation by targeting RORγt. Lithocholic acid 3-sulfate disodium can be used in the research of cholestatic liver diseases.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 64936-81-8
- Formula: C24H38Na2O6S
- Molecular Weight:500.60
-
Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-113308A
Taurolithocholic acid sodium salt
-
HY-133890A
Tauro-α-muricholic acid sodium
-
HY-W587430
Glycolithocholic acid 3-sulfate disodium
-
HY-133707
β-Muricholic acid
-
HY-137255
Taurolithocholic acid 3-sulfate disodium
-
HY-122141
Glycohyocholic acid
-
HY-116374A
Glycolithocholic acid sodium
-
HY-116374
Glycolithocholic acid
-
HY-121238
Hyocholic acid
-
HY-113478
3β-Ursodeoxycholic acid
-
HY-N0593A
Deoxycholic acid sodium salt
-
HY-N0593
Deoxycholic acid
Biological Activity
|
RORγt 1.7×10 |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CHO | EC50 |
>100 μM
Compound: 13, LCA-S
|
Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
|
[PMID: 18307294] |
| HEK-293T | EC50 |
>150 μM
Compound: SLCA
|
Agonist activity at VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
Agonist activity at VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
|
[PMID: 26774929] |
| HEK-293T | IC50 |
>50 μM
Compound: SLCA
|
Antagonist activity against VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as inhibition of 1,25-dihydroxyvitamin D3-induced SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
Antagonist activity against VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as inhibition of 1,25-dihydroxyvitamin D3-induced SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
|
[PMID: 26774929] |
Lithocholic acid 3-sulfate disodium binds to the purified human RORγt LBD with a Kd value of 1.7×10-4[1].
Lithocholic acid 3-sulfate disodium is a GPR39 agonist, with EC50 values of 41 μM and 42.4 μM in M39-20 and hGPR39-2 cells (in the absence of Zn2+), and 0.88 μM and 0.97 μM (in the presence of Zn2+)[2].
Lithocholic acid 3-sulfate disodium activates intracellular calcium signaling induced by mutant (H17A and H19A) GPR39 in 293T cells, with an EC50 value of 24 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 64936-81-8
-
Appearance Solid
-
Molecular Weight 500.60
-
Formula C24H38Na2O6S
-
Color White to off-white
-
SMILES
C[C@]1(CC2)[C@](CC[C@]1([H])[C@H](C)CCC(O[Na])=O)([H])[C@@](CC[C@]3([H])C[C@@H]4O[S](=O)(O[Na])=O)([H])[C@@]2([H])[C@]3(CC4)C
-
Synonyms
Sulfolithocholic acid disodium; LCAS disodium
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 25 mg/mL (49.94 mM; Need ultrasonic)
DMSO : 10 mg/mL (19.98 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (273 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.9976 mL | 9.9880 mL | 19.9760 mL | 49.9401 mL |
| 5 mM | 0.3995 mL | 1.9976 mL | 3.9952 mL | 9.9880 mL | |
| 10 mM | 0.1998 mL | 0.9988 mL | 1.9976 mL | 4.9940 mL | |
| 15 mM | 0.1332 mL | 0.6659 mL | 1.3317 mL | 3.3293 mL | |
| H2O | 20 mM | 0.0999 mL | 0.4994 mL | 0.9988 mL | 2.4970 mL |
| 25 mM | 0.0799 mL | 0.3995 mL | 0.7990 mL | 1.9976 mL | |
| 30 mM | 0.0666 mL | 0.3329 mL | 0.6659 mL | 1.6647 mL | |
| 40 mM | 0.0499 mL | 0.2497 mL | 0.4994 mL | 1.2485 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Lithocholic acid 3-sulfate disodium Related Classifications
HY-113238A Related Classifications
Powered by Bioz