TCS PrP Inhibitor 13
Based on 1 Customer Validation
TCS PrP Inhibitor 13, an antiprion agent, is a cellular prion protein (PrPC) inhibitor. TCS PrP Inhibitor 13, as a protease-resistant form of prion protein (PrP-res) accumulation inhibitor, shows an IC50 value of 3 nM in both ScN2a and F3 cell lines. TCS PrP Inhibitor 13 induces Schwannoma cells apoptosis.
For research use only. We do not sell to patients.
- Purity: 99.72%
- CAS No.: 34320-83-7
- Formula: C15H11N3O3
- Molecular Weight:281.27
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
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Biological Activity
IC50: 3 nM (PrPC in ScN2a and F3 cells)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| N2a #58 F3 | IC50 |
3 nM
Compound: 13
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Antiprion activity in F3 cells assessed as inhibition of protease-resistant prion protein accumulation
Antiprion activity in F3 cells assessed as inhibition of protease-resistant prion protein accumulation
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[PMID: 17850126] |
| ScN2a | IC50 |
3 nM
Compound: 13
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Antiprion activity in ScN2a cells assessed as inhibition of protease-resistant prion protein accumulation
Antiprion activity in ScN2a cells assessed as inhibition of protease-resistant prion protein accumulation
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[PMID: 17850126] |
TCS PrP Inhibitor 13 (0~500 μM; Schwannoma cells) significantly reduces levels of total ERK1/2, pAKT, total FAK, Cyclin D1, PrPC and significantly increases expression of cleaved caspase-3. TCS PrP Inhibitor 13 (Schwannoma cells) significantly reduces the number of proliferating, Ki67-positive cells and total cell number[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 34320-83-7
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Appearance Solid
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Molecular Weight 281.27
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Formula C15H11N3O3
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Color White to yellow
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SMILES
O=C1CC(C2=CC=C([N+]([O-])=O)C=C2)=NN1C3=CC=CC=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (355.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Kimata A, et al. New series of antiprion compounds: pyrazolone derivatives have the potent activity of inhibiting protease-resistant prion protein accumulation [published correction appears in J Med Chem. 2008 Mar 13;51(5):1503]. J Med Chem. 2007;50(21):5053-5056. [Content Brief]
[2]. Provenzano L, et al. Cellular prion protein (PrPC) in the development of Merlin-deficient tumours. Oncogene. 2017;36(44):6132-6142. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5553 mL | 17.7765 mL | 35.5530 mL | 88.8826 mL |
| 5 mM | 0.7111 mL | 3.5553 mL | 7.1106 mL | 17.7765 mL | |
| 10 mM | 0.3555 mL | 1.7777 mL | 3.5553 mL | 8.8883 mL | |
| 15 mM | 0.2370 mL | 1.1851 mL | 2.3702 mL | 5.9255 mL | |
| 20 mM | 0.1778 mL | 0.8888 mL | 1.7777 mL | 4.4441 mL | |
| 25 mM | 0.1422 mL | 0.7111 mL | 1.4221 mL | 3.5553 mL | |
| 30 mM | 0.1185 mL | 0.5926 mL | 1.1851 mL | 2.9628 mL | |
| 40 mM | 0.0889 mL | 0.4444 mL | 0.8888 mL | 2.2221 mL | |
| 50 mM | 0.0711 mL | 0.3555 mL | 0.7111 mL | 1.7777 mL | |
| 60 mM | 0.0593 mL | 0.2963 mL | 0.5926 mL | 1.4814 mL | |
| 80 mM | 0.0444 mL | 0.2222 mL | 0.4444 mL | 1.1110 mL | |
| 100 mM | 0.0356 mL | 0.1778 mL | 0.3555 mL | 0.8888 mL |
TCS PrP Inhibitor 13 Related Classifications
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