2. Metabolic Enzyme/Protease NF-κB Immunology/Inflammation Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis Reactive Oxygen Species Mitochondrial Metabolism
  4. Tempo

Tempo is a nitric oxide radical and a selective scavenger of ROS in mitochondria. Tempo is also an organocatalyst that disproportionates superoxide and oxidizes primary alcohols to aldehydes in a catalytic cycle. Tempo has mutagenic and antioxidant effects and can induceDNA strand breaks. Tempo also exerts cytotoxic and mutagenic properties in mouse lymphoma cells.

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Tempo Chemical Structure

Tempo Chemical Structure

CAS No. : 2564-83-2

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10 mM * 1 mL in DMSO
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Tempo:

Tempo Related Antibodies

Top Publications Citing Use of Products

    Tempo purchased from MedChemExpress. Usage Cited in: Br J Cancer. 2020 Jul;123(1):126-136.  [Abstract]

    Radiation inhibits AKT phosphorylation, and Tempo could alleviate the IDH2 knockdown-induced decrease in AKT phosphorylation in both the steady state and after ROS induction by radiation.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Tempo is a nitric oxide radical and a selective scavenger of ROS in mitochondria. Tempo is also an organocatalyst that disproportionates superoxide and oxidizes primary alcohols to aldehydes in a catalytic cycle. Tempo has mutagenic and antioxidant effects and can induceDNA strand breaks. Tempo also exerts cytotoxic and mutagenic properties in mouse lymphoma cells[1][2][3][4].

    In Vitro

    Tempo (1-5 mM; 4 h) can cause DNA damage in L5178Y cells and exert cytotoxicity and genotoxicity[2].
    Tempo (1-5 mM; 4 h) causes DNA breaks in mouse lymphoma cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tempo Related Antibodies

    Cell Cytotoxicity Assay[2]

    Cell Line: L5178Y cells and mouse lymphoma cells
    Concentration: 1, 2, 3, 4, 5 mM
    Incubation Time: 4 h
    Result: Exerted cytotoxicity and genotoxicity in L5178Y cells, and caused DNA-strand breaks in lymphoma cells.
    Molecular Weight

    156.25

    Formula

    C9H18NO
    CAS No.
    Appearance

    Solid

    Color

    Orange to reddish brown

    SMILES

    [O]N1C(C)(C)CCCC1(C)C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (640.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (640.00 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.4000 mL 32.0000 mL 64.0000 mL
    5 mM 1.2800 mL 6.4000 mL 12.8000 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (16.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (16.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.92%

    COA (248 KB) GC (202 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 6.4000 mL 32.0000 mL 64.0000 mL 160.0000 mL
    5 mM 1.2800 mL 6.4000 mL 12.8000 mL 32.0000 mL
    10 mM 0.6400 mL 3.2000 mL 6.4000 mL 16.0000 mL
    15 mM 0.4267 mL 2.1333 mL 4.2667 mL 10.6667 mL
    20 mM 0.3200 mL 1.6000 mL 3.2000 mL 8.0000 mL
    25 mM 0.2560 mL 1.2800 mL 2.5600 mL 6.4000 mL
    30 mM 0.2133 mL 1.0667 mL 2.1333 mL 5.3333 mL
    40 mM 0.1600 mL 0.8000 mL 1.6000 mL 4.0000 mL
    50 mM 0.1280 mL 0.6400 mL 1.2800 mL 3.2000 mL
    60 mM 0.1067 mL 0.5333 mL 1.0667 mL 2.6667 mL
    80 mM 0.0800 mL 0.4000 mL 0.8000 mL 2.0000 mL
    100 mM 0.0640 mL 0.3200 mL 0.6400 mL 1.6000 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Tempo Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tempo2564-83-2DNA/RNA SynthesisReactive Oxygen SpeciesMitochondrial MetabolismSODantioxidantpiperidinenitroxidecatalyticradicaldismutasescytotoxicitymutagenicDNA-strandInhibitorinhibitorinhibit

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