4-Amino-TEMPO
Based on 1 Customer Validation
4-Amino-TEMPO (4-Amino-2,2,6,6-tetramethylpiperidine-1-oxyl) is a stable nitroxide radical and N-nucleophile based on TEMPO. 4-Amino-TEMPO has superoxide dismutase-mimetic activity, can protect cells from oxidative damage, and has radioprotective effects. 4-Amino-TEMPO is widely used in fields such as biomedicine, materials science, and industrial production. 4-Amino-TEMPO can be used as a spin label to detect free radicals, an oxidation catalyst in industrial production, and an antioxidant stabilizer for polymers, among others.
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 14691-88-4
- Formula: C9H19N2O
- Molecular Weight:171.26
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
4-Amino-TEMPO is used as a spin label to detect various free radicals and the damage caused by these species. Determining it under physiological conditions can help study the mechanism of oxidative stress[1].
Dihydroxycoumarins can be used as fluorescent probes to detect 4-Amino-TEMPO with high sensitivity and selectivity. The interaction mechanism between them is proton transfer, and this detection is affected by the pH of the solution[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 14691-88-4
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Appearance <37°C Solid,>39°C Liquid
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Molecular Weight 171.26
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Formula C9H19N2O
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Color Pink to red
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SMILES
CC1(C)N([O])C(C)(C)CC(N)C1
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Synonyms
4-Amino-2,2,6,6-tetramethylpiperidine-1-oxyl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 200 mg/mL (1167.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (29.20 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (29.20 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.8391 mL | 29.1954 mL | 58.3908 mL | 145.9769 mL |
| 5 mM | 1.1678 mL | 5.8391 mL | 11.6782 mL | 29.1954 mL | |
| 10 mM | 0.5839 mL | 2.9195 mL | 5.8391 mL | 14.5977 mL | |
| 15 mM | 0.3893 mL | 1.9464 mL | 3.8927 mL | 9.7318 mL | |
| 20 mM | 0.2920 mL | 1.4598 mL | 2.9195 mL | 7.2988 mL | |
| 25 mM | 0.2336 mL | 1.1678 mL | 2.3356 mL | 5.8391 mL | |
| 30 mM | 0.1946 mL | 0.9732 mL | 1.9464 mL | 4.8659 mL | |
| 40 mM | 0.1460 mL | 0.7299 mL | 1.4598 mL | 3.6494 mL | |
| 50 mM | 0.1168 mL | 0.5839 mL | 1.1678 mL | 2.9195 mL | |
| 60 mM | 0.0973 mL | 0.4866 mL | 0.9732 mL | 2.4329 mL | |
| 80 mM | 0.0730 mL | 0.3649 mL | 0.7299 mL | 1.8247 mL | |
| 100 mM | 0.0584 mL | 0.2920 mL | 0.5839 mL | 1.4598 mL |
4-Amino-TEMPO Related Classifications
HY-W002004 Related Classifications
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