2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species (ROS) NO Synthase
  4. DMPO

DMPO is a cell permeable hydrophillic spin trap agent for superoxide detection.

For research use only. We do not sell to patients.

CAS No. : 3317-61-1

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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of DMPO:

DMPO Related Antibodies

Top Publications Citing Use of Products

    DMPO purchased from MedChemExpress. Usage Cited in: Chem Bio Eng. 2025 Dec 18.

    DMPO (5 μL, 10 M). ESR spectra of ·OH for ZnO2 and SNP/ZnO2 at various pH conditions.

    DMPO purchased from MedChemExpress. Usage Cited in: Chem Bio Eng. 2025 Dec 18.

    DMPO (5 μL, 10 M). ESR spectra of ·O2 for ZnO2 and SNP/ZnO2 at various pH conditions.

    View All NO Synthase Isoform Specific Products:

    View All Isoforms
    nNOS iNOS eNOS

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DMPO is a cell permeable hydrophillic spin trap agent for superoxide detection[1].

    In Vitro

    DMPO (100 μM, 24 h) attenuates SIN-1 (3-Morpholinosydnonimine (HY-126849)) (500 μM, 2 h)-mediated cytotoxicity and ROS generation in BAEC or HEK293 cells[3].
    DMPO (100 μM, 24 h) increases NO levels via increasing eNOS activity and phospho-eNOS levels in BAEC cells[3].
    DMPO (1-100 mM) inhibits FMLP and concanavalin A (HY-P2149) induced ·O2- secretion in neutrophils[4].
    DMPO (50 mM, 24 h) inhibits Lipopolysaccharides (HY-D1056)-triggered M1-linked pro-inflammatory cytokine (IL-1β, IL-6 and TNF-α) and NO production in RAW 264,6 cells[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DMPO Related Antibodies

    Western Blot Analysis[3]

    Cell Line: BAEC cells
    Concentration: 100 μM
    Incubation Time: 12 and 24 h
    Result: Increased p-eNOS and p-Akt level.
    In Vivo

    DMPO (10-100 mg/kg, i.p.) shows antinociceptive effect in rats subjected to Formalin induced hyperalgesia[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    113.16

    Formula

    C6H11NO
    CAS No.
    Appearance

    Solid-Liquid Mixture

    Color

    Colorless to off-white

    SMILES

    CC1(C)[N+]([O-])=CCC1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    H2O : 125 mg/mL (1104.63 mM; Need ultrasonic)

    DMSO : 100 mg/mL (883.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 8.8370 mL 44.1852 mL 88.3704 mL
    5 mM 1.7674 mL 8.8370 mL 17.6741 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (22.09 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (22.09 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.98%

    SDS (394 KB)

    COA (255 KB) HNMR (214 KB) RP-HPLC (244 KB) LCMS (88 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 8.8370 mL 44.1852 mL 88.3704 mL 220.9261 mL
    5 mM 1.7674 mL 8.8370 mL 17.6741 mL 44.1852 mL
    10 mM 0.8837 mL 4.4185 mL 8.8370 mL 22.0926 mL
    15 mM 0.5891 mL 2.9457 mL 5.8914 mL 14.7284 mL
    20 mM 0.4419 mL 2.2093 mL 4.4185 mL 11.0463 mL
    25 mM 0.3535 mL 1.7674 mL 3.5348 mL 8.8370 mL
    30 mM 0.2946 mL 1.4728 mL 2.9457 mL 7.3642 mL
    40 mM 0.2209 mL 1.1046 mL 2.2093 mL 5.5232 mL
    50 mM 0.1767 mL 0.8837 mL 1.7674 mL 4.4185 mL
    60 mM 0.1473 mL 0.7364 mL 1.4728 mL 3.6821 mL
    80 mM 0.1105 mL 0.5523 mL 1.1046 mL 2.7616 mL
    100 mM 0.0884 mL 0.4419 mL 0.8837 mL 2.2093 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    DMPO Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DMPO3317-61-1Reactive Oxygen Species (ROS)NO SynthaseNitric oxide synthasesNOSantinociceptiveeNOSROSBAEC cellInhibitorinhibitorinhibit

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