α,β-Methylene-ATP trisodium
Based on 1 publication(s) in Google Scholar
α,β-Methylene-ATP trisodium is an agonist of P2X1 and P2X3 receptors and can cross the blood-brain barrier. α,β-Methylene-ATP trisodium can trigger a reflex pressor response by activating P2X receptors in peripheral muscles and the central locus coeruleus (LC); this effect can be blocked by the P2X antagonist PPADS (HY-108960). α,β-Methylene-ATP trisodium also activates noradrenergic neurons in the central locus coeruleus, mediating antinociceptive effects; this effect can be attenuated by the locus coeruleus damaging agent DSP-4 (HY-103210/HY-121602). α,β-Methylene-ATP trisodium can be used to study the pathological mechanisms of neuropathic pain, cardiovascular reflex regulation, and antinociceptive effects of the central nervous system.
For research use only. We do not sell to patients.
- Purity: 99.0%
- CAS No.: 1343364-54-4
- Formula: C11H15N5Na3O12P3
- Molecular Weight:571.15
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) α,β-Methylene-ATP trisodium
MoreAll P2X Receptor Isoforms
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Biological Activity
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p2x1 Receptor |
P2X3 Receptor |
P2X7 Receptor |
Electrophysiological activity: α,β-Methylene-ATP trisodium (10 μM; 3 seconds) evokes a fast inward current in rat dorsal root ganglion (DRG) neurons, associated with P2X3 receptor activation. 10 μM Diinosine pentaphosphate (Ip5I) can significantly inhibit this inward current[1].
α,β-Methylene-ATP trisodium (10 μM; transient) enhances the amplitude of the current evoked by Acetylcholine (HY-B0282) in rat adrenal medullary chromaffin cells and increases the proportion of the current sensitive to α-conotoxin RgIA (HY-P5845)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
α,β-Methylene-ATP (10 nmol/rat; intracerebroventricular injection; single dose) trisodium produces an anti-mechanical nociceptive effect in Sprague-Dawley rats, which is significantly attenuated by pretreatment with DSP-4 (HY-121602/HY-103210) (10 mg/kg DSP-4; intraperitoneal injection)[3].
α,β-Methylene-ATP (0.1-1 nmol/side; bilateral microinjection into the locus coeruleus; single dose) trisodium increases the pain threshold of Sprague-Dawley rats in a dose-dependent manner, an effect that could be antagonized by co-injection of PPADS (HY-108960) (0.1-1 nmol/side)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Decerebrate cats[2]
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Dosage:5, 20, 50 µg/kg α,β-Methylene-ATP
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Administration:Left popliteal artery injection, single dose
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Result:Significantly increased mean arterial pressure. The pressor response was attenuated by sciatic nerve section and completely blocked by prior popliteal artery injection of PPADS (10 mg/kg), indicating involvement of P2X receptors in muscle afferents.
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Animal Model:Sprague-Dawley rats (male, 180-250 g, 6-8 weeks old)[3]
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Dosage:10 nmol/rat
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Administration:Intracerebroventricular injection, single dose
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Result:Elevated the mechanical nociceptive threshold by 63.6% at 5 min post-injection. Pretreatment with DSP-4 (50 mg/kg, i.p.), a noradrenergic neurotoxin, reduced the antinociceptive effect to baseline levels, suggesting involvement of locus coeruleus noradrenergic neurons.
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Animal Model:Sprague-Dawley rats (male, 180-250 g, 6-8 weeks old)[3]
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Dosage:0.1-1 nmol/side
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Administration:Bilateral locus coeruleus microinjection, single dose, no cycle
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Result:Dose-dependently increased the nociceptive threshold (peak at 5 min, 117.9% above control at 1 nmol/side).
Co-injection of PPADS (1 nmol/side) completely antagonized the antinociceptive effect, confirming P2X receptor-mediated mechanism.
Chemical Information
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CAS No. 1343364-54-4
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Appearance Solid
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Molecular Weight 571.15
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Formula C11H15N5Na3O12P3
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Color White to off-white
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SMILES
O=P(O)([O])OP(CP(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C3=NC=NC(N)=C3N=C2)O1)(O[Na])=O)(O[Na])=O.[Na]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Research (Wash D C)
2025 Oct 20:8:0932. PMID: 41122266
Solvent & Solubility
H2O : 125 mg/mL (218.86 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Arribas-Blázquez M, et al. Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain. Int J Mol Sci. 2019 Jan 3;20(1). [Content Brief]
[2]. Hanna RL, et al. alpha,beta-Methylene ATP elicits a reflex pressor response arising from muscle in decerebrate cats. J Appl Physiol (1985). 2002 Sep;93(3):834-41. [Content Brief]
[3]. Fukui M, et al. Involvement of locus coeruleus noradrenergic neurons in supraspinal antinociception by alpha,beta-methylene-ATP in rats. J Pharmacol Sci. 2004 Feb;94(2):153-60 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.7509 mL | 8.7543 mL | 17.5085 mL | 43.7713 mL |
| 5 mM | 0.3502 mL | 1.7509 mL | 3.5017 mL | 8.7543 mL | |
| 10 mM | 0.1751 mL | 0.8754 mL | 1.7509 mL | 4.3771 mL | |
| 15 mM | 0.1167 mL | 0.5836 mL | 1.1672 mL | 2.9181 mL | |
| 20 mM | 0.0875 mL | 0.4377 mL | 0.8754 mL | 2.1886 mL | |
| 25 mM | 0.0700 mL | 0.3502 mL | 0.7003 mL | 1.7509 mL | |
| 30 mM | 0.0584 mL | 0.2918 mL | 0.5836 mL | 1.4590 mL | |
| 40 mM | 0.0438 mL | 0.2189 mL | 0.4377 mL | 1.0943 mL | |
| 50 mM | 0.0350 mL | 0.1751 mL | 0.3502 mL | 0.8754 mL | |
| 60 mM | 0.0292 mL | 0.1459 mL | 0.2918 mL | 0.7295 mL | |
| 80 mM | 0.0219 mL | 0.1094 mL | 0.2189 mL | 0.5471 mL | |
| 100 mM | 0.0175 mL | 0.0875 mL | 0.1751 mL | 0.4377 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
α,β-Methylene-ATP trisodium Related Classifications
HY-108652 Related Classifications
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