Cat. No.: HY-136254

BzATP triethylammonium salt

15 Cited Publications

Customer Review

Based on 15 publication(s) in Google Scholar

BzATP triethylammonium salt acts as a P2X receptor agonist with pEC₅₀s of 8.74, 5.26, 7.10, 7.50, 6.19, 6.31, 5.33 for P2X1, P2X2, P2X3, P2X2/3, P2X4 and P2X7, respectively. BzATP triethylammonium salt is potent at P2X7 receptors with EC₅₀s of 3.6 μM and 285 μM for rat P2X7 and mouse P2X7, respectively.

For research use only. We do not sell to patients.

Purity: 99.19%
Formula: C₂₄H₂₄N₅O₁₅P₃.C₁₈H₄₅N₃
Molecular Weight:1018.97

Storage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

BzATP triethylammonium salt Chemical Structure

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (15)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

BzATP triethylammonium salt acts as a P2X receptor agonist with pEC₅₀s of 8.74, 5.26, 7.10, 7.50, 6.19, 6.31, 5.33 for P2X1, P2X2, P2X3, P2X2/3, P2X4 and P2X7, respectively^[1]. BzATP triethylammonium salt is potent at P2X7 receptors with EC₅₀s of 3.6 μM and 285 μM for rat P2X7 and mouse P2X7, respectively^[2].

IC₅₀ & Target

p2x1 Receptor

P2X3 Receptor

P2X4 Receptor

P2X7 Receptor

All P2X Receptor Isoforms :

In Vitro

BzATP (10-1000 μM; 24 h) promotes the proliferation and migration of U87 and U251 glioma cells^[3].
P2X7R protein expression is induced by BzATP (100 μM; 6-48 h) in human glioma cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[3]

Cell Line:

U87 and U251 glioma cells
Concentration:

5, 10, 50, 100, 500 and 1000 μM
Incubation Time:

2, 6, 12, 24, 48 and 72 hours
Result:

The proliferation of U87 and U251 glioma cell lines was significantly increased in the presence of 10-1000 uM and 100-1000 μM, respectively.
The peak of cell proliferation of both U87 and U251 cell lines was at 100 μM.
The optimal incubation time is 24 hours in both U87 and U251 cells lines.

Western Blot Analysis^[3]

Cell Line:

U87 and U251 glioma cells
Concentration:

100 μM
Incubation Time:

6-48 hours
Result:

Induced the upregulation of P2X7R.

In Vivo

BzATP (5 mg/kg) significantly promotes P2X7R expression in the intestines compared with intestines in the sham group and the control group after cecal ligation and puncture (CLP) induction^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male 2-month-old C57BL/6 mice (each weighing between 20 and 25 g)^[4]
Dosage:

5 mg/kg
Administration:

Injected through the intraperitoneal route
Result:

At 48 hours, mice in the treated group and control group exhibited mortalities of 91% and 86%, respectively.

Chemical Information

Appearance Solid
Molecular Weight 1018.97
Formula C₂₄H₂₄N₅O₁₅P₃.C₁₈H₄₅N₃
Color White to off-white
SMILES

O[C@@H]1[C@H](O[C@H]([C@@H]1OC(C(C=C2)=CC=C2C(C3=CC=CC=C3)=O)=O)N4C5=NC=NC(N)=C5N=C4)COP(OP(OP([O-])(O)=O)([O-])=O)([O-])=O.O=C(O[C@@H]6[C@H](O[C@H]([C@@H]6O)N7C8=NC=NC(N)=C8N=C7)COP(OP(OP([O-])(O)=O)([O-])=O)([O-])=O)C(C=C9)=CC=C9C(C%10=CC=CC=C%10)=O.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (15)

All In Vivo Efficacy StudyWBELISAIF

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Journal Impact Factor

Cell

Metabolite-gated vascular contractility switch: OXGR1 activation mechanism enables agonist therapy for rosacea erythema. [Abstract]

2026 Apr 2;189(7):1990-2006.e30. PMID: 41791372
Adv Sci (Weinh)

Microglia-Derived Interleukin-6 Triggers Astrocyte Apoptosis in the Hippocampus and Mediates Depression-Like Behavior. [Abstract]

2025 Mar;12(11):e2412556. PMID: 39888279

BzATP triethylammonium salt purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar;12(11):e2412556. [Abstract]

Drawing of ELISA technique for the detection of inflammatory cytokines secreted by BzATP (BzATP triethylammonium salt)‐activated cultured microglia (left). The concentration of IL‐6 released by microglia after BzATP (100 μM) treatment for 0 h, 4 h, 12 h, 24 h, and 36 h (right). Two‐way ANOVA. n = 4.
ACS Appl Mater Interfaces

The P2X7-Mediated Mitochondrial ROS as an Emerging Core Target of Tuftsin Nanoparticles in Severe Acute Pancreatitis Therapy via Regulating Mitophagy. [Abstract]

2025 Feb 5;17(5):7521-7538. PMID: 39854589
Chin Med

Qiming granules regulate Müller cell pyroptosis and the P2X7R/NLRP3 immune inflammatory pathway in diabetic retinopathy. [Abstract]

2026 Mar 16;21(1):89. PMID: 41840640
J Ethnopharmacol

Tangzu granule alleviate neuroinflammation in diabetic peripheral neuropathy by suppressing pyroptosis through P2X7R /NLRP3 signaling pathway. [Abstract]

2025 Jan 30;337(Pt 1):118792. PMID: 39251151
CNS Neurosci Ther

Astrocytic Connexin43 Channels Are Essential for Breathing Pattern Stabilization in the preBötzinger Complex. [Abstract]

2025 Nov;31(11):e70668. PMID: 41277876

BzATP triethylammonium salt purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Nov;31(11):e70668. [Abstract]

Representative traces showing the effect of injections of saline and BzATP (BzATP triethylammonium salt, 100 μM, 80 nL per injection) on PND activity.
Neurobiol Dis

S100A9 protein activates microglia and stimulates phagocytosis, resulting in synaptic and neuronal loss. [Abstract]

2025 Mar:206:106817. PMID: 39884585

BzATP triethylammonium salt purchased from MedChemExpress. Usage Cited in: Neurobiol Dis. 2025 Mar:206:106817. [Abstract]

Cell culture exposure to 500 nM S100A9 (prepared by protocol III) for 24 h was followed by 3 mM BzATP (BzATP triethylammonium salt) treatment for 1 h.
Int J Mol Sci

Quercetin Mitigates Lysophosphatidylcholine (LPC)-Induced Neutrophil Extracellular Traps (NETs) Formation through Inhibiting the P2X7R/P38MAPK/NOX2 Pathway. [Abstract]

2024 Aug 30;25(17):9411. PMID: 39273358

BzATP triethylammonium salt purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 30;25(17):9411. [Abstract]

Cells were treated by RPMI1640, LPC (100 μg/mL), LPC + Q (25 μmol/L), or LPC + Q + BzATP (BzATP triethylammonium salt, 100 µmol/L) for 3 h. Representative immunoblots probed for H3cit, P38MAPK, p-P38MAPK, NOX2, MPO, P2X7R, and β-Actin are shown.

BzATP triethylammonium salt purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 30;25(17):9411. [Abstract]

BzATP (BzATP triethylammonium salt, 100 µmol/L; 3 h). The level of NETs marker MPO-DNA in the supernatant was detected by ELISA.

BzATP triethylammonium salt purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 30;25(17):9411. [Abstract]

BzATP (BzATP triethylammonium salt, 100 µmol/L; 3 h). The formation of NETs was identified by labeling with H3cit and observed by fluorescence microscopy (scale bar, 50 µm).
Int Immunopharmacol

BzATP reverses ferroptosis-induced gut microbiota disorders in collagen-induced arthritis mice. [Abstract]

2023 Sep 13;124(Pt A):110885. PMID: 37713784
Inflammation

Vitexin Alleviates Kainic Acid-Induced Seizure Through Inhibiting P2X7R/NLRP3 Signaling Pathway. [Abstract]

2025 Jun 30. PMID: 40586851
ACS Omega

Mechanism of Luteolin in the Treatment of Primary Sjogren's Syndrome: a Study Based on Systems Biology and Cell Experiments. [Abstract]

2025 Apr 15;10(16):16339-16354. PMID: 40321503
Neuropharmacology

Hypericin alleviates high glucose-induced ferroptosis in cultured rat satellite glial cells via targeting P2X7R to activate the Akt/GSK3β/Nrf2 axis. [Abstract]

2026 Nov 1:298:111050. PMID: 42214457
J Endocrinol Invest

Pannexin-1 regulation of ATP release promotes the invasion of pituitary adenoma. [Abstract]

2025 Feb;48(2):317-332. PMID: 39527372
Neuroscience

Electroacupuncture reduces microglial pyroptosis via P2X7R/NLRP3 axis in the rat model of asphyxial cardiac arrest and cardiopulmonary resuscitation. [Abstract]

2025 Mar 27:570:27-37. PMID: 39952315
Neuroscience

P2X7 Receptor in microglia contributes to propofol-induced unconsciousness by regulating synaptic plasticity in mice. [Abstract]

2023 Jul 15:523:157-172. PMID: 37211083

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (49.07 mM; Need ultrasonic)

DMSO : 25 mg/mL (24.53 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

0.9814 mL

4.9069 mL

9.8138 mL

5 mM

0.1963 mL

0.9814 mL

1.9628 mL

10 mM

0.0981 mL

0.4907 mL

0.9814 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. B R Bianchi, et al. Pharmacological characterization of recombinant human and rat P2X receptor subtypes. Eur J Pharmacol. 1999 Jul 2;376(1-2):127-38. [Content Brief]

[2]. Mark T Young, et al. Amino acid residues in the P2X7 receptor that mediate differential sensitivity to ATP and BzATP. Mol Pharmacol. 2007 Jan;71(1):92-100. [Content Brief]

[3]. Zhenhua Ji, et al. Involvement of P2X 7 Receptor in Proliferation and Migration of Human Glioma Cells. Biomed Res Int. 2018 Jan 9;2018:8591397. [Content Brief]

[4]. Xiuwen Wu, et al. Systemic blockade of P2X7 receptor protects against sepsis-induced intestinal barrier disruption. Sci Rep. 2017 Jun 29;7(1):4364. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	0.9814 mL	4.9069 mL	9.8138 mL	24.5346 mL
	5 mM	0.1963 mL	0.9814 mL	1.9628 mL	4.9069 mL
	10 mM	0.0981 mL	0.4907 mL	0.9814 mL	2.4535 mL
	15 mM	0.0654 mL	0.3271 mL	0.6543 mL	1.6356 mL
	20 mM	0.0491 mL	0.2453 mL	0.4907 mL	1.2267 mL
H₂O	25 mM	0.0393 mL	0.1963 mL	0.3926 mL	0.9814 mL
	30 mM	0.0327 mL	0.1636 mL	0.3271 mL	0.8178 mL
	40 mM	0.0245 mL	0.1227 mL	0.2453 mL	0.6134 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

BzATP triethylammonium salt

15 Cited Publications

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BzATP triethylammonium salt

Bioactive Compound Library Max

Bioactive Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Anti-Cancer Compound Library

Covalent Screening Library

Neurotransmitter Receptor Compound Library

Targeted Diversity Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Ion Channel Compound Library

Multi-Target Compound Library

Covalent Screening Library Plus

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BzATP triethylammonium salt

All P2X Receptor Isoforms

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

BzATP triethylammonium salt

Bioactive Compound Library Max

Bioactive Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Anti-Cancer Compound Library

Covalent Screening Library

Neurotransmitter Receptor Compound Library

Targeted Diversity Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Ion Channel Compound Library

Multi-Target Compound Library

Covalent Screening Library Plus

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BzATP triethylammonium salt

All P2X Receptor Isoforms

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

BzATP triethylammonium salt Related Classifications

HY-136254 Related Classifications