1-Oleoyl-sn-glycero-3-phosphocholine
Based on 1 publication(s) in Google Scholar
1-Oleoyl-sn-glycero-3-phosphocholine (18:1 Lyso PC), a lysophospholipid, is a GPR82 inhibitor. 1-Oleoyl-sn-glycero-3-phosphocholine abrogates constitutive Gi-coupled GPR82 activity, shifts active/inactive equilibrium to inactive, suppresses Gi protein activation, increases cAMP production, and decreases GTPγS binding to Gαi proteins. 1-Oleoyl-sn-glycero-3-phosphocholine contributes to adipocyte lipolysis regulation.1-Oleoyl-sn-glycero-3-phosphocholine exhibits reduced serum levels in mouse models of steatohepatitis, linked to hepatic Lpcat 1-4 up-regulation.
For research use only. We do not sell to patients.
- Purity : 99.58%
- CAS No.: 19420-56-5
- Formula: C26H52NO7P
- Molecular Weight:521.67
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 1-Oleoyl-sn-glycero-3-phosphocholine
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
1-Oleoyl-sn-glycero-3-phosphocholine (1-10 μM; 30 min) increases cAMP production in Forskolin (HY-15371)-treated CHO-GPR82 cells in a concentration-dependent manner[1].
1-Oleoyl-sn-glycero-3-phosphocholine (3.3-10 μM; 10 min pre-incubation; 30 min [35S]GTPγS binding) concentration-dependently inhibits the constitutive [35S]GTPγS activity of GPR82 in CHO-GPR82 cell membrane fractions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
1-Oleoyl-sn-glycero-3-phosphocholine shows that intraperitoneal injection of GalN into male ob/ob mice induces steatohepatitis and causes a significant reduction in serum 1-Oleoyl-sn-glycero-3-phosphocholine levels relative to saline-injected controls[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 19420-56-5
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Appearance Solid
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Molecular Weight 521.67
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Formula C26H52NO7P
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Color White to off-white
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SMILES
CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)O
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Synonyms
18:1 Lyso PC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
Ethanol : 50 mg/mL (95.85 mM; Need ultrasonic)
H2O : 33.33 mg/mL (63.89 mM; Need ultrasonic)
DMSO : 10 mg/mL (19.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (1.92 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (1.92 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (647 KB)
- English - EN (647 KB)
- Français - FR (647 KB)
- Deutsch - DE (647 KB)
- Norwegian - NO (647 KB)
- Español - ES (647 KB)
- Swedish - SV (647 KB)
- Italian - IT (647 KB)
- Korean - KR (647 KB)
- Portuguese - PT (647 KB)
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Handling Instructions (2659 KB)
References
[1]. Yasuda D, et al. Inverse agonism of lysophospholipids with cationic head groups at Gi-coupled receptor GPR82. Eur J Pharmacol. 2023;954:175893. [Content Brief]
[2]. Tanaka N, et al. Disruption of phospholipid and bile acid homeostasis in mice with nonalcoholic steatohepatitis. Hepatology. 2012;56(1):118-129. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O / Ethanol | 1 mM | 1.9169 mL | 9.5846 mL | 19.1692 mL | 47.9230 mL |
| 5 mM | 0.3834 mL | 1.9169 mL | 3.8338 mL | 9.5846 mL | |
| 10 mM | 0.1917 mL | 0.9585 mL | 1.9169 mL | 4.7923 mL | |
| 15 mM | 0.1278 mL | 0.6390 mL | 1.2779 mL | 3.1949 mL | |
| H2O / Ethanol | 20 mM | 0.0958 mL | 0.4792 mL | 0.9585 mL | 2.3962 mL |
| 25 mM | 0.0767 mL | 0.3834 mL | 0.7668 mL | 1.9169 mL | |
| 30 mM | 0.0639 mL | 0.3195 mL | 0.6390 mL | 1.5974 mL | |
| 40 mM | 0.0479 mL | 0.2396 mL | 0.4792 mL | 1.1981 mL | |
| 50 mM | 0.0383 mL | 0.1917 mL | 0.3834 mL | 0.9585 mL | |
| 60 mM | 0.0319 mL | 0.1597 mL | 0.3195 mL | 0.7987 mL | |
| Ethanol | 80 mM | 0.0240 mL | 0.1198 mL | 0.2396 mL | 0.5990 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
1-Oleoyl-sn-glycero-3-phosphocholine Related Classifications
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