1-Oleoyl-sn-glycero-3-phosphocholine (Synonyms: 18:1 Lyso PC)

Cat. No.: HY-W140439 Purity: 99.58%: Data Sheet
COA

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1-Oleoyl-sn-glycero-3-phosphocholine (18:1 Lyso PC), a lysophospholipid, is a GPR82 inhibitor. 1-Oleoyl-sn-glycero-3-phosphocholine abrogates constitutive Gi-coupled GPR82 activity, shifts active/inactive equilibrium to inactive, suppresses Gi protein activation, increases cAMP production, and decreases GTPγS binding to Gαi proteins. 1-Oleoyl-sn-glycero-3-phosphocholine contributes to adipocyte lipolysis regulation.1-Oleoyl-sn-glycero-3-phosphocholine exhibits reduced serum levels in mouse models of steatohepatitis, linked to hepatic Lpcat 1-4 up-regulation.

For research use only. We do not sell to patients.

1-Oleoyl-sn-glycero-3-phosphocholine Chemical Structure

CAS No. : 19420-56-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Other Forms of 1-Oleoyl-sn-glycero-3-phosphocholine:

1-Oleoyl-sn-glycero-3-phosphocholine-d₇ In-stock
1-Oleoyl-sn-glycero-3-phosphocholine (Standard) Get quote

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Description

In Vitro

1-Oleoyl-sn-glycero-3-phosphocholine (1-10 μM; 30 min) increases cAMP production in Forskolin (HY-15371)-treated CHO-GPR82 cells in a concentration-dependent manner^[1].
1-Oleoyl-sn-glycero-3-phosphocholine (3.3-10 μM; 10 min pre-incubation; 30 min [³⁵S]GTPγS binding) concentration-dependently inhibits the constitutive [³⁵S]GTPγS activity of GPR82 in CHO-GPR82 cell membrane fractions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

1-Oleoyl-sn-glycero-3-phosphocholine shows that feeding male C57BL/6NCr mice an MCD diet causes a time-dependent reduction in serum 1-Oleoyl-sn-glycero-3-phosphocholine levels as NASH develops^[2].
1-Oleoyl-sn-glycero-3-phosphocholine shows that intraperitoneal injection of GalN into male ob/ob mice induces steatohepatitis and causes a significant reduction in serum 1-Oleoyl-sn-glycero-3-phosphocholine levels relative to saline-injected controls^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

521.67

Formula

C₂₆H₅₂NO₇P

CAS No.

19420-56-5

Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : 50 mg/mL (95.85 mM; Need ultrasonic)

H₂O : 33.33 mg/mL (63.89 mM; Need ultrasonic)

DMSO : 10 mg/mL (19.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9169 mL	9.5846 mL	19.1692 mL
5 mM	0.3834 mL	1.9169 mL	3.8338 mL
10 mM	0.1917 mL	0.9585 mL	1.9169 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (1.92 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (1.92 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 1 mg/mL (1.92 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.58%

Select Batch:

Data Sheet (268 KB) SDS (647 KB)

COA (239 KB) HNMR (242 KB) RP-HPLC (243 KB) MS (72 KB)

Handling Instructions (2659 KB)

References

[1]. Yasuda D, et al. Inverse agonism of lysophospholipids with cationic head groups at Gi-coupled receptor GPR82. Eur J Pharmacol. 2023;954:175893.

[2]. Tanaka N, et al. Disruption of phospholipid and bile acid homeostasis in mice with nonalcoholic steatohepatitis. Hepatology. 2012;56(1):118-129.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O / Ethanol	1 mM	1.9169 mL	9.5846 mL	19.1692 mL	47.9230 mL
	5 mM	0.3834 mL	1.9169 mL	3.8338 mL	9.5846 mL
	10 mM	0.1917 mL	0.9585 mL	1.9169 mL	4.7923 mL
	15 mM	0.1278 mL	0.6390 mL	1.2779 mL	3.1949 mL
H₂O / Ethanol	20 mM	0.0958 mL	0.4792 mL	0.9585 mL	2.3962 mL
	25 mM	0.0767 mL	0.3834 mL	0.7668 mL	1.9169 mL
	30 mM	0.0639 mL	0.3195 mL	0.6390 mL	1.5974 mL
	40 mM	0.0479 mL	0.2396 mL	0.4792 mL	1.1981 mL
	50 mM	0.0383 mL	0.1917 mL	0.3834 mL	0.9585 mL
	60 mM	0.0319 mL	0.1597 mL	0.3195 mL	0.7987 mL
Ethanol	80 mM	0.0240 mL	0.1198 mL	0.2396 mL	0.5990 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

1-Oleoyl-sn-glycero-3-phosphocholine Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1-Oleoyl-sn-glycero-3-phosphocholine19420-56-518:1 Lyso PCEndogenous MetabolitecAMPGPR82primary hepatocytesCHO-GPR82 cellsob/ob miceadipocyte lipolysisGi proteinnonalcoholic steatohepatitisHEK-GPR82 cellsC57BL/6NCr miceInhibitorinhibitorinhibit

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