1-Stearoyl-sn-glycero-3-phosphocholine
Based on 1 publication(s) in Google Scholar
1-Stearoyl-sn-glycero-3-phosphocholine is a lysophosphatidylcholine that inhibits HDAC3 activity and phosphorylation of STAT3 in K562 cells. 1-Stearoyl-sn-glycero-3-phosphocholine induces apoptosis and exhibits anticancer activity in chronic myelogenous leukemia (CML) K562 cells.
For research use only. We do not sell to patients.
- Purity: 99.53%
- CAS No.: 19420-57-6
- Formula: C26H54NO7P
- Molecular Weight:523.68
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 1-Stearoyl-sn-glycero-3-phosphocholine
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
HDAC3 |
p-STAT3 |
In Vitro
1-Stearoyl-sn-glycero-3-phosphocholine (10-80 μM; 24-72 h) significantly reduces the viability of K562, HL-60, U937 and MDA - MB-231 cells[1].
1-Stearoyl-sn-glycero-3-phosphocholine (80 μM; 24 h) induces apoptosis in K562, U937 and HL-60 cells, and significantly increases the sub-G1 cell population in K562 cells[1].
1-Stearoyl-sn-glycero-3-phosphocholine (80 μM; 24 h) induces the cleavage of caspase-9, caspase 3 and PARP, enhances the levels of acetyl - histone H3 at Lys 18 and 23, reduces the expression of HDAC3, inhibits the phosphorylation of STAT3 at Ser 727 and Tyr 705, and downregulates the expression of survival genes such as Cyclin D1, Cyclin E, Bcl-xL, Bcl-2 and survivin in K562 cells[1].
K562
80 μM
24 h
Induced apoptosis.
K562
80 μM
24 h
The sub-G1 cell population was significantly increased.
K562
80 μM
24 h
Downregulated the expression of survival genes such as Cyclin D1, Cyclin E, Bcl-xL, Bcl-2, and survivin.
K562
0 μM, 10 μM, 20 μM, 40 μM, 80 μM
24 h
Induced the cleavage of caspase-9, caspase 3, and PARP. It also enhanced the levels of acetyl - histone H3 at Lys 18 and 23, reduced the expression of HDAC3, inhibited the phosphorylation of STAT3 at Ser 727 and Tyr 705.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:K562 cells
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Concentration:0 μM, 10 μM, 20 μM, 40 μM, 80 μM
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Incubation Time:24, 48 and 72 h
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Result:Decreased cell viability in a concentration-dependent manner.
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Cell Line:K562 cells
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Concentration:80 μM
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Incubation Time:24 h
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Result:Induced apoptosis.
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Cell Line:K562 cells
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Concentration:80 μM
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Incubation Time:24 h
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Result:The sub-G1 cell population was significantly increased.
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Cell Line:K562 cells
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Concentration:80 μM
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Incubation Time:24 h
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Result:Downregulated the expression of survival genes such as Cyclin D1, Cyclin E, Bcl-xL, Bcl-2, and survivin.
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Cell Line:K562 cells
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Concentration:0 μM, 10 μM, 20 μM, 40 μM, 80 μM
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Incubation Time:24 h
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Result:Induced the cleavage of caspase-9, caspase 3, and PARP. It also enhanced the levels of acetyl-histone H3 at Lys 18 and 23, reduced the expression of HDAC3, inhibited the phosphorylation of STAT3 at Ser 727 and Tyr 705.
Chemical Information
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CAS No. 19420-57-6
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Appearance Solid
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Molecular Weight 523.68
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Formula C26H54NO7P
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Color White to off-white
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SMILES
CCCCCCCCCCCCCCCCCC(OC[C@@H](O)COP(OCC[N+](C)(C)C)([O-])=O)=O
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Synonyms
18:0 Lyso-PC; LPC(18:0)
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Structure Classification
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Initial Source
Hirudo nipponica
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
H2O : 25 mg/mL (47.74 mM; Need ultrasonic)
Ethanol : 12.5 mg/mL (23.87 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.39 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.39 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.39 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / H2O | 1 mM | 1.9096 mL | 9.5478 mL | 19.0956 mL | 47.7391 mL |
| 5 mM | 0.3819 mL | 1.9096 mL | 3.8191 mL | 9.5478 mL | |
| 10 mM | 0.1910 mL | 0.9548 mL | 1.9096 mL | 4.7739 mL | |
| 15 mM | 0.1273 mL | 0.6365 mL | 1.2730 mL | 3.1826 mL | |
| 20 mM | 0.0955 mL | 0.4774 mL | 0.9548 mL | 2.3870 mL | |
| H2O | 25 mM | 0.0764 mL | 0.3819 mL | 0.7638 mL | 1.9096 mL |
| 30 mM | 0.0637 mL | 0.3183 mL | 0.6365 mL | 1.5913 mL | |
| 40 mM | 0.0477 mL | 0.2387 mL | 0.4774 mL | 1.1935 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
1-Stearoyl-sn-glycero-3-phosphocholine Related Classifications
HY-125771 Related Classifications
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