3-Carboxyumbelliferyl acetate
3-Carboxyumbelliferyl acetate (compound ACC) is a fluorescent substrate that can be used to detect the activity of the Escherichia coli polysaccharide deacetylase PgaB, with a Km of 1.2 mM. 3-Carboxyumbelliferyl acetate undergoes acetate hydrolysis under the catalysis of E. coli PgaB. 3-Carboxyumbelliferyl acetate can be used in studies related to PgaB enzymatic activity and the mechanism of bacterial biofilm formation.
For research use only. We do not sell to patients.
- CAS No.: 81017-23-4
- Formula: C12H8O6
- Molecular Weight:248.19
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
PgaB 1.2 mM (Km) |
In Vitro
3-Carboxyumbelliferyl acetate (compound ACC) (up to 10 mM) serves as a soluble and stable substrate for purified E. coli PgaB, and is applicable for assaying the catalytic activity of PgaB, with a Km value of 1.2 mM and a kcat value of 0.013 s−1[1].
3-Carboxyumbelliferyl acetate serves as a competitive substrate in assays evaluating inhibitors of E. coli PgaB, which can confirm the competitive binding of active inhibitors to the PgaB active site[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 81017-23-4
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Molecular Weight 248.19
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Formula C12H8O6
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SMILES
O=C(O)C1=CC=2C=CC(OC(=O)C)=CC2OC1=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Fungal Biofilm Culture
Fungal biofilm culture is an in vitro method for growing surface-attached fungal communities, most commonly Candida albicans, on abiotic substrates such as polystyrene wells, silicone elastomer, or polymethylmethacrylate; the assay models adhesion, proliferation, filamentation, extracellular-matrix-associated maturation, and dispersion. Biofilm output can be read by optical density at 600 nm for adherent biomass, XTT reduction for metabolic activity, CFU recovery for viable attached or dispersed cells, and microscopy for architecture.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
3-Carboxyumbelliferyl acetate Related Classifications
HY-187021 Related Classifications
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