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Cat. No.: HY-185273

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3'-O-(2-Nitrobenzyl)-dGTP

3'-O-(2-Nitrobenzyl)-dGTP is a reversible terminator. 3'-O-(2-Nitrobenzyl)-dGTP can be recognized and incorporated by DNA polymerases, thereby temporarily terminating DNA primer extension; after the 2-nitrobenzyl blocking group is removed via laser irradiation, a free 3'-OH can be regenerated to allow subsequent polymerase-mediated extension. 3'-O-(2-Nitrobenzyl)-dGTP can be used in DNA sequencing studies.

For research use only. We do not sell to patients.

CAS No.: 959755-49-8
Formula: C₁₇H₂₁N₆O₁₅P₃
Molecular Weight:642.30

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

3'-O-(2-Nitrobenzyl)-dGTP Chemical Structure

CAS No.: 959755-49-8

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Biological Activity

Description

IC₅₀ & Target

DNA Polymerase

All DNA/RNA Synthesis Isoforms :

In Vitro

3'-O-(2-Nitrobenzyl)-dGTP (100 pmol) can be efficiently incorporated by 9°N polymerase (exo-) A485L/Y409V to terminate primer extension, and its 3'-O-(2-nitrobenzyl) group can be completely removed by 30 s of 355 nm laser, thereby regenerating a free 3'-OH for subsequent extension cycles using the same target reagent^[1].
3'-O-(2-Nitrobenzyl)-dGTP (200 pmol) acts as a reversible terminator in pyrosequencing, enabling accurate single-base resolution identification of guanine bases in homopolymeric DNA regions immobilized on Sepharose microspheres^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 959755-49-8
Molecular Weight 642.30
Formula C₁₇H₂₁N₆O₁₅P₃
SMILES

OP(OC[C@H]1O[C@@H](N2C(NC(N)=NC3=O)=C3N=C2)C[C@@H]1OCC4=CC=CC=C4[N+]([O-])=O)(OP(OP(O)(O)=O)(O)=O)=O
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

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Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wu J, et al. 3'-O-modified nucleotides as reversible terminators for pyrosequencing. Proc Natl Acad Sci U S A. 2007;104(42):16462-16467. [Content Brief]

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How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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3'-O-(2-Nitrobenzyl)-dGTP

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

3'-O-(2-Nitrobenzyl)-dGTP Related Classifications

HY-185273 Related Classifications