7,12-Diketolithocholic acid
Based on 1 Customer Validation
7,12-Diketolithocholic acid (7,12-Diketo-LCA) is a bile acid, which can be produced in the host-gut microbial co-metabolism of the bile acid pool.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 517-33-9
- Formula: C24H36O5
- Molecular Weight:404.54
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Chemical Information
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CAS No. 517-33-9
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Appearance Solid
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Molecular Weight 404.54
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Formula C24H36O5
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Color White to off-white
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SMILES
O[C@@H](C[C@@](CC([C@@]1([H])[C@]2([H])CC[C@@H]([C@]23C)[C@H](C)CCC(O)=O)=O)4[H])CC[C@]4(C)[C@@]1([H])CC3=O
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Synonyms
7,12-Dioxolithocholic acid; 3-Hydroxy-7,12-diketocholanoic acid; 7,12-Diketo-LCA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : ≥ 100 mg/mL (247.19 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4719 mL | 12.3597 mL | 24.7194 mL | 61.7986 mL |
| 5 mM | 0.4944 mL | 2.4719 mL | 4.9439 mL | 12.3597 mL | |
| 10 mM | 0.2472 mL | 1.2360 mL | 2.4719 mL | 6.1799 mL | |
| 15 mM | 0.1648 mL | 0.8240 mL | 1.6480 mL | 4.1199 mL | |
| 20 mM | 0.1236 mL | 0.6180 mL | 1.2360 mL | 3.0899 mL | |
| 25 mM | 0.0989 mL | 0.4944 mL | 0.9888 mL | 2.4719 mL | |
| 30 mM | 0.0824 mL | 0.4120 mL | 0.8240 mL | 2.0600 mL | |
| 40 mM | 0.0618 mL | 0.3090 mL | 0.6180 mL | 1.5450 mL | |
| 50 mM | 0.0494 mL | 0.2472 mL | 0.4944 mL | 1.2360 mL | |
| 60 mM | 0.0412 mL | 0.2060 mL | 0.4120 mL | 1.0300 mL | |
| 80 mM | 0.0309 mL | 0.1545 mL | 0.3090 mL | 0.7725 mL | |
| 100 mM | 0.0247 mL | 0.1236 mL | 0.2472 mL | 0.6180 mL |
7,12-Diketolithocholic acid Related Classifications
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