2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. 7-Ketolithocholic acid

7-Ketolithocholic acid  (Synonyms: 3α-Hydroxy-7-oxo-5β-cholanic acid)

Cat. No.: HY-W018512 Purity: 99.93%
COA
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Handling Instructions Technical Support

7-Ketolithocholic acid (3α-Hydroxy-7-oxo-5β-cholanic acid), a bile acid, can be absorbed and suppresses endogenous bile acid production and biliary cholesterol secretion.

For research use only. We do not sell to patients.

CAS No. : 4651-67-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g In-stock
10 g   Get quote  
50 g   Get quote  

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of 7-Ketolithocholic acid:

7-Ketolithocholic acid Related Antibodies

Top Publications Citing Use of Products

    7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121.

    7-Ketolithocholic acid (7-KA; 50 mg/kg; PO; Once every two days, for a total of 5 times) significantly mitigated the reduction in colon length in the DSS-induced colitis model mice.

    7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121.

    7-Ketolithocholic acid (7-KA; 50 mg/kg; PO; Once every two days, for a total of 5 times) showed the levels of inflammatory factors such as IL-18, IL-17, and IL-1b were significantly greater in the DSS alone group than in the DSS and 7-KA group.

    7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121.

    7-Ketolithocholic acid (7-KA; 50 mg/kg; PO; Once every two days, for a total of 5 times) improved DSS-induced mucosal gland damage and increased the protein levels of ZO-1 and Ki67.

    7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121.

    7-Ketolithocholic acid (7-KA; 50 mg/kg; PO; Once every two days, for a total of 5 times) significantly increased CD4 and FOXP3 expression in intestinal tissues.

    7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121.

    7-Ketolithocholic acid (7-KA; 0, 10, 20, 30, 40, 50 μg/mL; 8 h/ 50 μg/mL; 0, 2,4, 6, 8, 10 h) increased FOXP3 protein expression in a time- and concentration-dependent manner in 293T cells.

    7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121.

    7-Ketolithocholic acid (7-KA; 0, 10, 20, 30, 40, 50 μg/mL; 8 h/ 50 μg/mL; 0, 2,4, 6, 8, 10 h)showed no effect on the FOXP3 mRNA expression in 293T cells.

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    7-Ketolithocholic acid (3α-Hydroxy-7-oxo-5β-cholanic acid), a bile acid, can be absorbed and suppresses endogenous bile acid production and biliary cholesterol secretion[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Bile acids are main components of mammalian bile. They are steroid carboxylic acids that synthesized from cholesterol in liver tissue[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    7-Ketolithocholic acid Related Antibodies
    Molecular Weight

    390.56

    Formula

    C24H38O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])C(C[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (256.04 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5604 mL 12.8021 mL 25.6043 mL
    5 mM 0.5121 mL 2.5604 mL 5.1209 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.93%

    SDS (396 KB)

    COA (250 KB) HNMR (292 KB) RP-HPLC (236 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5604 mL 12.8021 mL 25.6043 mL 64.0107 mL
    5 mM 0.5121 mL 2.5604 mL 5.1209 mL 12.8021 mL
    10 mM 0.2560 mL 1.2802 mL 2.5604 mL 6.4011 mL
    15 mM 0.1707 mL 0.8535 mL 1.7070 mL 4.2674 mL
    20 mM 0.1280 mL 0.6401 mL 1.2802 mL 3.2005 mL
    25 mM 0.1024 mL 0.5121 mL 1.0242 mL 2.5604 mL
    30 mM 0.0853 mL 0.4267 mL 0.8535 mL 2.1337 mL
    40 mM 0.0640 mL 0.3201 mL 0.6401 mL 1.6003 mL
    50 mM 0.0512 mL 0.2560 mL 0.5121 mL 1.2802 mL
    60 mM 0.0427 mL 0.2134 mL 0.4267 mL 1.0668 mL
    80 mM 0.0320 mL 0.1600 mL 0.3201 mL 0.8001 mL
    100 mM 0.0256 mL 0.1280 mL 0.2560 mL 0.6401 mL
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    7-Ketolithocholic acid Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    7-Ketolithocholic acid4651-67-63α-Hydroxy-7-oxo-5β-cholanic acidEndogenous MetabolitebileacidendogenousbiliarycholesterolsecretionInhibitorinhibitorinhibit

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