7-Ketolithocholic acid
Based on 8 publication(s) in Google Scholar
7-Ketolithocholic acid (3α-Hydroxy-7-oxo-5β-cholanic acid), a bile acid, can be absorbed and suppresses endogenous bile acid production and biliary cholesterol secretion.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 4651-67-6
- Formula: C24H38O4
- Molecular Weight:390.56
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Bioactive Compound Library Max
HY-L181
-
Bioactive Compound Library
HY-L001
-
Metabolism/Protease Compound Library
HY-L012
-
Natural Product Library
HY-L021
-
Human Endogenous Metabolite Compound Library
HY-L030
-
Lipid Compound Library
HY-L043
-
FDA Approved & Pharmacopeial Drug Library
HY-L066
-
Food-Sourced Compound Library
HY-L094
-
Human Metabolite Library
HY-L123
-
Cysteine Targeted Covalent Library
HY-L153
-
Animal-Sourced Natural Product Library
HY-L157
-
MCE Bioactive Compound Library
HY-L001V
-
Natural Product Library Plus
HY-L021P
-
Natural Product and Natural Product-Like Compound Library
HY-L021M
-
Fatty Acids Compound Library
HY-L182
-
RO5 Drug-like Natural Product Library
HY-L200
-
High-Throughput Bioactive Compound Library
HY-L205
-
High-Throughput Natural Product Library
HY-L206
-
Mass Spectrometry Human Endogenous Metabolite Library
HY-L207
-
Bile Acids Library
HY-L208
-
Mass Spectrometry Human Metabolite Library
HY-L215
-
Mass Spectrometry Natural Product Library
HY-L262
Publications Citing Use of MedChemExpress (MCE) 7-Ketolithocholic acid
More- Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]
- Microbiome. 2024 Jul 17;12(1):128. [Abstract]
- NPJ Biofilms Microbiomes. 2026 May 22. [Abstract]
- J Pharm Anal. 2025 Apr;15(4):101121. [Abstract]
- J Transl Med. 2026 Jun 18. [Abstract]
- J Agric Food Chem. 2026 Apr 22;74(15):12069-12086. [Abstract]
- Biomedicines. 2025 Apr 4;13(4):874. [Abstract]
- bioRxiv. 2026 May 5:2026.04.30.721772. [Abstract]
Customer Validation & Images
-
In Vivo Efficacy Study
-
ELISA
-
Histological Imaging/Staining
-
IF
-
WB
All Endogenous Metabolite Isoforms
More
Biological Activity
|
Human Endogenous Metabolite |
Bile acids are main components of mammalian bile. They are steroid carboxylic acids that synthesized from cholesterol in liver tissue[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 4651-67-6
-
Appearance Solid
-
Molecular Weight 390.56
-
Formula C24H38O4
-
Color White to off-white
-
SMILES
C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])C(C[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C)=O
-
Synonyms
3α-Hydroxy-7-oxo-5β-cholanic acid
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (8)
-
Journal Impact Factor
-
Most Recent
-
Cell Host Microbe
A gut microbiota-bile acid axis promotes intestinal homeostasis upon aspirin-mediated damage. [Abstract]2024 Feb 14;32(2):191-208.e9. PMID: 38237593 -
Microbiome
Parabacteroides distasonis regulates the infectivity and pathogenicity of SVCV at different water temperatures. [Abstract]2024 Jul 17;12(1):128. PMID: 39020382 -
NPJ Biofilms Microbiomes
Parabacteroides goldsteinii and its metabolite 7-KLCA attenuate endometriosis via TGR5 to reprogram macrophages by modulating the PPARγ/GPR132 axis. [Abstract]2026 May 22. PMID: 42173876 -
J Pharm Anal
Thalidomide mitigates Crohn's disease colitis by modulating gut microbiota, metabolites, and regulatory T cell immunity. [Abstract]2025 Apr;15(4):101121. PMID: 40309194
7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121. [Abstract]
7-Ketolithocholic acid (7-KA; 50 mg/kg; PO; Once every two days, for a total of 5 times) significantly mitigated the reduction in colon length in the DSS-induced colitis model mice.
7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121. [Abstract]
7-Ketolithocholic acid (7-KA; 50 mg/kg; PO; Once every two days, for a total of 5 times) showed the levels of inflammatory factors such as IL-18, IL-17, and IL-1b were significantly greater in the DSS alone group than in the DSS and 7-KA group.
7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121. [Abstract]
7-Ketolithocholic acid (7-KA; 50 mg/kg; PO; Once every two days, for a total of 5 times) improved DSS-induced mucosal gland damage and increased the protein levels of ZO-1 and Ki67.
7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121. [Abstract]
7-Ketolithocholic acid (7-KA; 50 mg/kg; PO; Once every two days, for a total of 5 times) significantly increased CD4 and FOXP3 expression in intestinal tissues.
7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121. [Abstract]
7-Ketolithocholic acid (7-KA; 0, 10, 20, 30, 40, 50 μg/mL; 8 h/ 50 μg/mL; 0, 2,4, 6, 8, 10 h) increased FOXP3 protein expression in a time- and concentration-dependent manner in 293T cells.
7-Ketolithocholic acid purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Apr;15(4):101121. [Abstract]
7-Ketolithocholic acid (7-KA; 0, 10, 20, 30, 40, 50 μg/mL; 8 h/ 50 μg/mL; 0, 2,4, 6, 8, 10 h)showed no effect on the FOXP3 mRNA expression in 293T cells.
-
J Transl Med
Chronic proton pump inhibitor exposure aggravates intestinal injury by impairing intestinal stem cell self-renewal through the microbiota-7-ketolithocholic acid Axis. [Abstract]2026 Jun 18. PMID: 42316284 -
J Agric Food Chem
Vitamin D 3 Metabolite-Enhanced Hepatic VDR-FXR Binding Attenuates Bile Acid Dysregulation-Induced Diarrhea in Weaned Piglets. [Abstract]2026 Apr 22;74(15):12069-12086. PMID: 41952052 -
Biomedicines
2025 Apr 4;13(4):874. PMID: 40299495 -
bioRxiv
2026 May 5:2026.04.30.721772. PMID: 42146479
Solvent & Solubility
DMSO : ≥ 100 mg/mL (256.04 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
[1]. Choucair I, et al. Quantification of bile acids: a mass spectrometry platform for studying gut microbe connectionto metabolic diseases. J Lipid Res. 2020 Feb;61(2):159-177. [Content Brief]
[2]. Salen G, et al. Effect of 7-ketolithocholic acid on bile acid metabolism in humans. Gastroenterology. 1982 Aug;83(2):341-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5604 mL | 12.8021 mL | 25.6043 mL | 64.0107 mL |
| 5 mM | 0.5121 mL | 2.5604 mL | 5.1209 mL | 12.8021 mL | |
| 10 mM | 0.2560 mL | 1.2802 mL | 2.5604 mL | 6.4011 mL | |
| 15 mM | 0.1707 mL | 0.8535 mL | 1.7070 mL | 4.2674 mL | |
| 20 mM | 0.1280 mL | 0.6401 mL | 1.2802 mL | 3.2005 mL | |
| 25 mM | 0.1024 mL | 0.5121 mL | 1.0242 mL | 2.5604 mL | |
| 30 mM | 0.0853 mL | 0.4267 mL | 0.8535 mL | 2.1337 mL | |
| 40 mM | 0.0640 mL | 0.3201 mL | 0.6401 mL | 1.6003 mL | |
| 50 mM | 0.0512 mL | 0.2560 mL | 0.5121 mL | 1.2802 mL | |
| 60 mM | 0.0427 mL | 0.2134 mL | 0.4267 mL | 1.0668 mL | |
| 80 mM | 0.0320 mL | 0.1600 mL | 0.3201 mL | 0.8001 mL | |
| 100 mM | 0.0256 mL | 0.1280 mL | 0.2560 mL | 0.6401 mL |
7-Ketolithocholic acid Related Classifications
HY-W018512 Related Classifications
Powered by Bioz