1. Cell Cycle/DNA Damage
    Anti-infection
  2. DNA/RNA Synthesis
    Bacterial
    Antibiotic
  3. 7-Aminoactinomycin D

7-Aminoactinomycin D (Synonyms: 7-AAD)

Cat. No.: HY-D1020 Purity: ≥99.0%
Handling Instructions

7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects.

For research use only. We do not sell to patients.

7-Aminoactinomycin D Chemical Structure

7-Aminoactinomycin D Chemical Structure

CAS No. : 7240-37-1

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1 mg USD 230 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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1 Publications Citing Use of MCE 7-Aminoactinomycin D

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  • Customer Review

Description

7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects[1][2][3].

In Vitro

7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. 7-Aminoactinomycin D staining with 5 μg/mL, 10 μg/mL, and 20 μg/mL, but not with 1 μg/mL, is suitable for quantification of apoptosis in flow cytometry[1].
7-Aminoactinomycin D is frequently used to stain and exclude dead cells in flow cytometry at low concentrations (0.5-5 μg/mL). At higher concentrations (10-20 μg/mL), 7-Aminoactinomycin D has also been used to distinguish between viable cells (7-AADnegative) and apoptotic cells (7-AADdim) or dead cells (7-AADbright) using the fact that permeability of the cell membrane, and hence fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1270.43

Formula

C62H87N13O16

CAS No.
SMILES

O=C(N[[email protected]](C(N1[[email protected]@](C(N(C)CC(N(C)[[email protected]@H](C(C)C)C(O[[email protected]@H]2C)=O)=O)=O)([H])CCC1)=O)C(C)C)[[email protected]]2NC(C(C(C(OC3=C(C)C(N)=C4)=C5C)=NC3=C4C(N[[email protected]@H]6C(N[[email protected]](C(N7[[email protected]@](C(N(C)CC(N(C)[[email protected]@H](C(C)C)C(O[[email protected]@H]6C)=O)=O)=O)([H])CCC7)=O)C(C)C)=O)=O)=C(N)C5=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (78.71 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7871 mL 3.9357 mL 7.8714 mL
5 mM 0.1574 mL 0.7871 mL 1.5743 mL
10 mM 0.0787 mL 0.3936 mL 0.7871 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: ≥99.0%

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7-Aminoactinomycin D
Cat. No.:
HY-D1020
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