7-Aminoactinomycin D for apoptosis staining in flow cytometry

  • Anal Biochem. 2012 Oct 1;429(1):79-81. doi: 10.1016/j.ab.2012.07.005.
Nadine C L Zembruski  1 ,  Vanessa Stache ,  Walter E Haefeli ,  Johanna Weiss
Affiliations
  • 1. Department of Clinical Pharmacology and Pharmacoepidemiology, University of Heidelberg, 69120 Heidelberg, Germany.
PMID: 22796502 DOI: 10.1016/j.ab.2012.07.005
Abstract

7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. Several staining protocols using 7-AAD have been described, but data on the influence of the 7-AAD concentration on the readout are not available. Therefore, we compared the results obtained by staining with 1, 5, 10, and 20μg/ml 7-AAD for 20min with the PE-Annexin V Apoptosis Detection Kit and Cell Death Detection ELISA(PLUS) in lymphocytes and CEM human leukemia cells. The results showed that 7-AAD staining with 5, 10, and 20μg/ml, but not with 1μg/ml, is suitable for quantification of Apoptosis in flow cytometry.

Products