1. Anti-infection Cytoskeleton Cell Cycle/DNA Damage Autophagy Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation NF-κB Protein Tyrosine Kinase/RTK
  2. Parasite Microtubule/Tubulin Autophagy Apoptosis Reactive Oxygen Species VEGFR HIF/HIF Prolyl-Hydroxylase Antibiotic Bacterial
  3. Albendazole

Albendazole  (Synonyms: SKF-62979)

Cat. No.: HY-B0223 Purity: 99.00%
COA Handling Instructions

Albendazole (SKF-62979) is an orally active and broad-spectrum parasiticide with high effectiveness and low host toxicity, is used for the research of gastrointestinal parasites in humans and animals. Albendazole induces apoptosis and autophagy in cancer cells. Albendazole also inhibits tubulin polymerization and HIF-1α, VEGF expression, has antioxidant activity, and inhibits the glycolytic process in cancer cells.

For research use only. We do not sell to patients.

Albendazole Chemical Structure

Albendazole Chemical Structure

CAS No. : 54965-21-8

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
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Solid
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Customer Review

Based on 3 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Albendazole (SKF-62979) is an orally active and broad-spectrum parasiticide with high effectiveness and low host toxicity, is used for the research of gastrointestinal parasites in humans and animals. Albendazole induces apoptosis and autophagy in cancer cells. Albendazole also inhibits tubulin polymerization and HIF-1α, VEGF expression, has antioxidant activity, and inhibits the glycolytic process in cancer cells[1][2][3][4][5].

IC50 & Target

parasites, tubulin[1][2].
HIF-1α, VEGF[3].

In Vitro

Albendazole (100, 500, 1000 nM; 1, 3, or 5 days) inhibits cell proliferation in a dose-dependent manner[1].
? Albendazole (100, 250, 500, 1000 nM; 3 days) arrests SKHEP-1 cells at both G0–G1 (250, 500 nM) and G2-M (1000 nM) phases of the cycle[1].
? Albendazole (5 μM; 24, 36 h) mainly induces early apoptosis in HCT-15 cells and late apoptosis in HCT-1 16, HT29, SW480 cells accompanied with cleavage of PARP and caspase-3 in a time-dependent manner[2].
? Albendazole (5 μM; 24, 36 h) induces autophagy via increasing autophagy-related protein (such as LC3, Atg7, p-beclin-1, and beclin-1) expression level in HCT-15, HCT-1 16, HT29, and SW480 cells[2].
? Albendazole (500 nM, 24 h) inhibits hypoxia-induced HIF-1α expression and VEGF expression in A549 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: SKHEP-1 cells
Concentration: 100, 500, 1000 nM
Incubation Time: 1, 3, or 5 days
Result: Inhibited cell proliferation in a dose-dependent manner.

Cell Cycle Analysis[1]

Cell Line: SKHEP-1 HCC cells
Concentration: 100, 250, 500, 1000 nM
Incubation Time: 3 days
Result: Showed dose-dependent effect on the cell cycle kinetics.

Apoptosis Analysis[2]

Cell Line: HCT-15, HCT-1 16, HT29, SW480 cells
Concentration: 5 µM
Incubation Time: 24, 36 h
Result: Promoted apoptosis in colon cancer cells.

Cell Autophagy Assay[2]

Cell Line: HCT-15, HCT-1 16, HT29, SW480 cells
Concentration: 5 µM
Incubation Time: 24, 36 h
Result: Induced autophagy in colon cancer cells.

Western Blot Analysis[2]

Cell Line: HCT-15, HCT-1 16, HT29, SW480 cells
Concentration: 5 µM
Incubation Time: 12, 24, 36 h
Result: Induced apoptosis-related protein (PARP, caspase-3) and autophagy-related protein (such as LC3, Atg7, p-beclin-1, and beclin-1) expression level in a time-dependent manner.
Increased phosphorylation of different MAPKs (AMPK, ERK, JNK, p38) and ULK1 protein in a time dependent manner, and up-regulated the activation of different MAPKs.
Caused the activation of both MAPK and AMPK pathways.

Western Blot Analysis[3]

Cell Line: A549 cells
Concentration: 500 nM
Incubation Time: 24 h
Result: Inhibited hypoxia-induced HIF-1α expression and VEGF expression in A549 cells.
In Vivo

Albendazole (10 mg/kg; i.g.; once a day for 30 days) reduces Echinococcus granulosus cyst weights in mice[4].
? Albendazole (300 mg/kg; p.o.; per day in two divided dose for 20 days) profoundly suppresses tumor growth in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c mice (10-week-age; Echinococcus granulosus infection model)[4].
Dosage: 10 mg/kg
Administration: Oral gavage; once a day for 30 days.
Result: Reduced Echinococcus granulosus cyst weights.
Animal Model: Male BALB/c Nu/Nu mice (6 to 10-week-old; inoculated subcutaneously with SKHEP-1)[1].
Dosage: 50, 150, 300 mg/kg
Administration: Oral administration; per day in two divided dose for 20 days.
Result: Profoundly suppressed tumor growth in vivo.
Clinical Trial
Molecular Weight

265.33

Formula

C12H15N3O2S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OC)NC1=NC2=CC=C(SCCC)C=C2N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (75.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7689 mL 18.8445 mL 37.6889 mL
5 mM 0.7538 mL 3.7689 mL 7.5378 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2 mg/mL (7.54 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2 mg/mL (7.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.00%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7689 mL 18.8445 mL 37.6889 mL 94.2223 mL
5 mM 0.7538 mL 3.7689 mL 7.5378 mL 18.8445 mL
10 mM 0.3769 mL 1.8844 mL 3.7689 mL 9.4222 mL
15 mM 0.2513 mL 1.2563 mL 2.5126 mL 6.2815 mL
20 mM 0.1884 mL 0.9422 mL 1.8844 mL 4.7111 mL
25 mM 0.1508 mL 0.7538 mL 1.5076 mL 3.7689 mL
30 mM 0.1256 mL 0.6281 mL 1.2563 mL 3.1407 mL
40 mM 0.0942 mL 0.4711 mL 0.9422 mL 2.3556 mL
50 mM 0.0754 mL 0.3769 mL 0.7538 mL 1.8844 mL
60 mM 0.0628 mL 0.3141 mL 0.6281 mL 1.5704 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Albendazole
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