Cat. No.: HY-B1289

Cetylpyridinium chloride monohydrate

Name: Cetylpyridinium chloride monohydrate
Brand: MedChemExpress
SKU: HY-B1289
Price: 30.00 USD
Availability: InStock
Rating: 5 (9 reviews)

(Synonyms: Hexadecylpyridinium chloride monohydrate)

9 Cited Publications

Customer Review

Based on 9 publication(s) in Google Scholar

Cetylpyridinium chloride monohydrate is a cationic quaternary ammonium compound used in some types of mouthwashes, toothpastes, and throat and nasal sprays as an antiseptic that kills bacteria and other microorganisms. Effectively prevents plaque and reduces gingivitis.

For research use only. We do not sell to patients.

Purity: 99.84%
CAS No.: 6004-24-6
Formula: C₂₁H₄₀ClNO
Molecular Weight:358.00

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Cetylpyridinium chloride monohydrate Chemical Structure

CAS No.: 6004-24-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (9)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	CC₅₀	0.66 μM Compound: CETYLPYRIDINIUM CHLORIDE	Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay	10.21203/rs.3.rs-23951/v1
Caco-2	IC₅₀	0.62 μM Compound: CETYLPYRIDINIUM CHLORIDE	Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging	10.21203/rs.3.rs-23951/v1

In Vitro

Cetylpyridinium chloride monohydrate (7.5-50 µg/mL, 5 minutes) disrupts the influenza viral envelope in a dose-dependent manner, leading to structural damage, increased membrane permeability, and nucleoprotein release^[2].
Cetylpyridinium chloride monohydrate (10-250 µg/mL, 48 hours) exhibits dose-dependent cytotoxicity in MDCK cells, with a CC₅₀ of 96 µg/mL^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:

Madin-Darby Canine Kidney (MDCK) cells
Concentration:

10-250 µg/mL
Incubation Time:

10 minutes at room temperature, followed by 48 hours incubation at 37°C
Result:

Led to a decrease in cell viability as the concentration increased. The 50% cytotoxic concentration (CC₅₀) was 96 µg/mL.

In Vivo

Cetylpyridinium chloride monohydrate (5 µL of 0.1% Cetylpyridinium chloride monohydrate, p.o., twice daily for 5 days) enhances survival and reduces influenza-associated morbidity and mortality in C57BL/6 mice infected with mouse-adapted Influenza A (PR8 strain)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 mice infected with mouse-adapted Influenza A (PR8 strain)^[2]
Dosage:

5 µL of ARMS-1 (Cetylpyridinium chloride monohydrate-based formulation, 0.1% Cetylpyridinium chloride monohydrate w/v)
Administration:

Oral gavage (p.o.), twice daily for 5 days
Result:

Led to higher body weight maintenance in mice and significantly increased survival rates compared to the control group.

Chemical Information

CAS No. 6004-24-6
Appearance Solid
Molecular Weight 358.00
Formula C₂₁H₄₀ClNO
Color White to off-white
SMILES

CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1.O.[Cl-]
Synonyms

Hexadecylpyridinium chloride monohydrate
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (9)

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Journal Impact Factor

Adv Sci (Weinh)

Hyperlipidemia Aggravates Alveolar Bone Loss via Periodontal Ligament Stem Cell Ferroptosis Through GSK3β Dependent Ubiquitin-Mediated NRF2 Degradation. [Abstract]

2026 Jun;13(36):e75157. PMID: 41945797
Chem Eng J

2025 Nov 12;525:170824.
J Control Release

Bioinspired nanomicelles with octopus-like adhesion for microenvironmental reprogramming in periodontitis. [Abstract]

2026 Apr 10:392:114649. PMID: 41570864
Cell Death Dis

Oxidative phosphorylation promotes vascular calcification in chronic kidney disease. [Abstract]

2022 Mar 11;13(3):229. PMID: 35277475
ACS Biomater Sci Eng

Electrospun Nanofibers Loaded with Concentrated Growth Factors and Nanohydroxyapatite for the Healing of Alveolar Bone in Tooth Extraction Wounds. [Abstract]

2025 Sep 2. PMID: 40891613
Inflammation

4-Octyl Itaconate Attenuates Postmenopausal Osteoporosis by Inhibiting Ferroptosis and Enhancing Osteogenesis via the Nrf2 Pathway. [Abstract]

2025 Feb 22. PMID: 39984770
ACS Omega

Urinary Bladder Matrix as a Guide Bone Regeneration Barrier Membrane for Inhibiting Cell Invasion and Promoting Bone Formation. [Abstract]

2025 Jul 30;10(31):34831-34843. PMID: 40821524
ACS Omega

Discovery of PLpro and Mpro Inhibitors for SARS-CoV-2. [Abstract]

2023 Jun 14;8(25):22603-22612. PMID: 37387790
iScience

2025 Nov 6.

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (139.66 mM; Need ultrasonic)

DMSO : 20 mg/mL (55.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7933 mL

13.9665 mL

27.9330 mL

5 mM

0.5587 mL

2.7933 mL

5.5866 mL

10 mM

0.2793 mL

1.3966 mL

2.7933 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2 mg/mL (5.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2 mg/mL (5.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2 mg/mL (5.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (279.33 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (785 KB)
- English - EN (785 KB)
- Français - FR (785 KB)
- Deutsch - DE (785 KB)
- Norwegian - NO (785 KB)
- Español - ES (785 KB)
- Swedish - SV (785 KB)
- Italian - IT (785 KB)
- Korean - KR (785 KB)
- Portuguese - PT (785 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Datta S, et al. In Vitro Evaluation of Mitochondrial Function and Estrogen Signaling in Cell Lines Exposed to the Antiseptic Cetylpyridinium Chloride. Environ Health Perspect. 2017 Aug 22;125(8):087015. [Content Brief]

[2]. Popkin DL, et al. Cetylpyridinium Chloride (CPC) Exhibits Potent, Rapid Activity Against Influenza Viruses in vitro and in vivo. Pathog Immun. 2017;2(2):252-269. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	2.7933 mL	13.9665 mL	27.9330 mL	69.8324 mL
	5 mM	0.5587 mL	2.7933 mL	5.5866 mL	13.9665 mL
	10 mM	0.2793 mL	1.3966 mL	2.7933 mL	6.9832 mL
	15 mM	0.1862 mL	0.9311 mL	1.8622 mL	4.6555 mL
	20 mM	0.1397 mL	0.6983 mL	1.3966 mL	3.4916 mL
	25 mM	0.1117 mL	0.5587 mL	1.1173 mL	2.7933 mL
	30 mM	0.0931 mL	0.4655 mL	0.9311 mL	2.3277 mL
	40 mM	0.0698 mL	0.3492 mL	0.6983 mL	1.7458 mL
	50 mM	0.0559 mL	0.2793 mL	0.5587 mL	1.3966 mL
H₂O	60 mM	0.0466 mL	0.2328 mL	0.4655 mL	1.1639 mL
	80 mM	0.0349 mL	0.1746 mL	0.3492 mL	0.8729 mL
	100 mM	0.0279 mL	0.1397 mL	0.2793 mL	0.6983 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

Keywords

Cetylpyridinium chloride monohydrate

9 Cited Publications

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Cetylpyridinium chloride monohydrate

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