Dimetacrine
Based on 1 Customer Validation
Dimetacrine is a useful antidepressant effect, and can be used for the research of various types of depression.
For research use only. We do not sell to patients.
- Purity: 98.08%
- CAS No.: 4757-55-5
- Formula: C20H26N2
- Molecular Weight:294.43
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 4757-55-5
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Appearance Viscous liquid
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Molecular Weight 294.43
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Formula C20H26N2
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Color Colorless to light yellow
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SMILES
CN(C)CCCN1C2=CC=CC=C2C(C)(C)C3=C1C=CC=C3
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Synonyms
Dimethacrine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (339.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (16.98 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Adult male mongrel dogs weighing 8 to 13 kg are used and anesthetized with sodium pentobarbital, 35 mg/kg i.v. Respiratory movement is measured by an accordion-type pneumograph placed around the thoraco-abdominal region and a pressure transducer. For the experiments on single i.v. injections, the increasing doses of Dimetacrine, 0.1, 0.3, 1, 3, 10 and 30 mg/kg i.v., until cardiac arrest occurs, are given into the cannulated left cephalic vein and are flushed with 1 mL of physiological saline. The changes in arterial blood pressure and heart rate caused by each dose are estimated for IMP, AMT and Dimetacrine[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3964 mL | 16.9820 mL | 33.9639 mL | 84.9098 mL |
| 5 mM | 0.6793 mL | 3.3964 mL | 6.7928 mL | 16.9820 mL | |
| 10 mM | 0.3396 mL | 1.6982 mL | 3.3964 mL | 8.4910 mL | |
| 15 mM | 0.2264 mL | 1.1321 mL | 2.2643 mL | 5.6607 mL | |
| 20 mM | 0.1698 mL | 0.8491 mL | 1.6982 mL | 4.2455 mL | |
| 25 mM | 0.1359 mL | 0.6793 mL | 1.3586 mL | 3.3964 mL | |
| 30 mM | 0.1132 mL | 0.5661 mL | 1.1321 mL | 2.8303 mL | |
| 40 mM | 0.0849 mL | 0.4245 mL | 0.8491 mL | 2.1227 mL | |
| 50 mM | 0.0679 mL | 0.3396 mL | 0.6793 mL | 1.6982 mL | |
| 60 mM | 0.0566 mL | 0.2830 mL | 0.5661 mL | 1.4152 mL | |
| 80 mM | 0.0425 mL | 0.2123 mL | 0.4245 mL | 1.0614 mL | |
| 100 mM | 0.0340 mL | 0.1698 mL | 0.3396 mL | 0.8491 mL |
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