2. Induced Disease Models Products GPCR/G Protein MAPK/ERK Pathway NF-κB Neuronal Signaling Metabolic Enzyme/Protease Immunology/Inflammation PI3K/Akt/mTOR Apoptosis
  3. Immunology and Inflammatory Disease Models Digestive System Disease Models Akt p38 MAPK Reactive Oxygen Species (ROS) Histamine Receptor Endogenous Metabolite Apoptosis
  4. Gastrointestinal Inflammation and Ulcer Models Gastrointestinal Disease Models
  5. Gastric Ulcer Models Duodenal Ulcer Models
  6. Histamine phosphate

Histamine phosphate  (Synonyms: Histamine diphosphate)

Cat. No.: HY-A0129 Purity: 99.34%
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Histamine phosphate is the agonist for histamine receptor and a vasodilator. Histamine phosphate is an organic nitrogen compound that participates in local immune responses, regulates intestinal physiological functions, and acts as a neurotransmitter. Histamine phosphate affects p38 MAPK/Akt signaling pathway, exhibits antitumor, antioxidant and anti-inflammatory activities. Histamine phosphate can be used in the research of acute myeloid leukemia, malignant melanoma, and renal cell carcinoma.

For research use only. We do not sell to patients.

CAS No. : 51-74-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
500 mg In-stock
5 g In-stock
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Based on 20 publication(s) in Google Scholar

Other Forms of Histamine phosphate:

Histamine phosphate Related Antibodies

Top Publications Citing Use of Products

    Histamine phosphate purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Jul 15;39(13):e70778.  [Abstract]

    Changes in HRH1 gene expression in F11 cells after treatment with 10−7 mM histamine for 24 h, as measured by RT–qPCR.

    Histamine phosphate purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Jul 15;39(13):e70778.  [Abstract]

    Changes in HRH1 protein expression in F11 cells after treatment with 10−7 mM histamine for 24 h, as detected by western blotting.

    Histamine phosphate purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Jul 15;39(13):e70778.  [Abstract]

    Increase in the Ca2+ peak (DF/F0) in F11 cells from the control, histamine (10−7 mM for 24 h), and histamine+DLT groups (n = 3 in each group).

    Histamine phosphate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Mar;10(7):e2203869.  [Abstract]

    Confocal microscopy of mitochondrial Ca2+ levels in HepG2 transfected with GCaMP6m. Histamine (100 μM) was applied to stimulate mitochondrial Ca2+ accumulation, and the 475/410 fluorescence ratio was analyzed.

    Histamine phosphate purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2019 Nov 15;10:1380.  [Abstract]

    Effects of histamine (0.1%, 20 µL/site, intradermal injection) on scratching behavior in mice.

    View All Akt Isoform Specific Products:

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    Akt Akt1 Akt2 Akt3

    View All p38 MAPK Isoform Specific Products:

    View All Isoforms
    p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

    View All Histamine Receptor Isoform Specific Products:

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    H1 Receptor H2 Receptor H3 Receptor H4 Receptor Histamine Receptor

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Histamine phosphate is the agonist for histamine receptor and a vasodilator. Histamine phosphate is an organic nitrogen compound that participates in local immune responses, regulates intestinal physiological functions, and acts as a neurotransmitter. Histamine phosphate affects p38 MAPK/Akt signaling pathway, exhibits antitumor, antioxidant and anti-inflammatory activities. Histamine phosphate can be used in the research of acute myeloid leukemia, malignant melanoma, and renal cell carcinoma[1][2][3][4][5][6][7].

    In Vitro

    Histamine phosphate (0.01-10 μM, 72 h) exhibits a bidirectional regulatory effect on the proliferation of MDA-MB-231, arrests the cell cycle at G2/M phase, induces the formation of lipid droplet, induces differentiation and apoptosis in MDA-MB-231[4].
    Histamine phosphate (10 μM, 24 h) increases the generation of ROS, especially H2O2, reduces the activity of antioxidant enzymes (such as catalase), thereby promoting radiosensitivity in MDA-MB-231[4].
    Histamine phosphate (1-100 μM, 12 h) inhibits the migration of microglia and the release of inflammatory mediator IL-1β in LPS (HY-D1056)-stimulated N9 mouse microglial, exhibits anti-inflammatory activity[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Histamine phosphate Related Antibodies

    Cell Proliferation Assay[4]

    Cell Line: MDA-MB-231
    Concentration: 0.01-10 µM
    Incubation Time: 72 h
    Result: Promoted cell proliferation at 10 µM, inhibited cell proliferation at 0.01 µM.

    Apoptosis Analysis[4]

    Cell Line: MDA-MB-231
    Concentration: 10 µM
    Incubation Time: 48 h
    Result: Induced apoptosis.
    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    Histamine (0.05-100 μg/mouse, icv, single dose) exhibits analgesic effect at high doses of histamine (50-100 μg/mouse), causes hyperalgesia at low doses (0.1-1 μg/mouse) in mouse model[3].
    Histamine dihydrochloride (62.5-2000 μg; Intravaginal injection) can exert a similar effect to estrogen in ovariectomized mice, causing a decrease in intravaginal tetrazolium, an increase in epithelial growth, and keratinization[6].
    Histamine can be used in animal modeling to create gastrointestinal ulcer models[1].

    Induction of Gastrointestinal Ulcer[1]
    Background
    Histamine can cause increased gastric acid secretion, decreased mucus production, reflux of pancreatic juice, poor gastric blood flow, and thus gastric ulcers. Stress can cause increased gastrointestinal motility, making the gastric folds more susceptible to damage when exposed to acid.
    Specific Modeling Methods
    Guinea Pig: male • albino • Administration: 5 mg/kg • i.p. • single dose
    Note
    (1) No eating or drinking is allowed 24 hours before the experiment.
    (2) The animals are sacrificed 2 hours after histamine injection, and treated with 1% formalin to check for gastric ulcers.
    Modeling Indicators
    The ulcers were punctate or elongated. After the model was dissected, the ulcer index (the sum of the lengths of the lesions) was measured under a microscope to be 3.4 mm.
    Correlated Product(s): Serotonin (HY-B1473A); Reserpine (HY-N0480)
    Opposite Product(s): Cimetidine (HY-14289)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Mouse model[3]
    Dosage: 0.05-100 μg/mouse
    Administration: icv, single dose
    Result: Prolonged the licking latency at the dose of 50 and 100 μg/mouse.
    Shortened the licking latency at the dose of 0.1-1 μg/mouse.
    Animal Model: Ovariectomized female mice of the SW strain[6]
    Dosage: 62.5-2000 μg
    Administration: Intravaginal injection
    Result: Stimulated a vaginal response similar to that caused by estrogen, resulting in a decrease in intravaginal tetrazolium, increased epithelial growth, and keratinization.
    Molecular Weight

    307.14

    Formula

    C5H15N3O8P2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NCCC1=CNC=N1.O=P(O)(O)O.O=P(O)(O)O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (325.58 mM; Need ultrasonic)

    DMSO : 1.43 mg/mL (4.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2558 mL 16.2792 mL 32.5584 mL
    5 mM 0.6512 mL 3.2558 mL 6.5117 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (162.79 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.2558 mL 16.2792 mL 32.5584 mL 81.3961 mL
    H2O 5 mM 0.6512 mL 3.2558 mL 6.5117 mL 16.2792 mL
    10 mM 0.3256 mL 1.6279 mL 3.2558 mL 8.1396 mL
    15 mM 0.2171 mL 1.0853 mL 2.1706 mL 5.4264 mL
    20 mM 0.1628 mL 0.8140 mL 1.6279 mL 4.0698 mL
    25 mM 0.1302 mL 0.6512 mL 1.3023 mL 3.2558 mL
    30 mM 0.1085 mL 0.5426 mL 1.0853 mL 2.7132 mL
    40 mM 0.0814 mL 0.4070 mL 0.8140 mL 2.0349 mL
    50 mM 0.0651 mL 0.3256 mL 0.6512 mL 1.6279 mL
    60 mM 0.0543 mL 0.2713 mL 0.5426 mL 1.3566 mL
    80 mM 0.0407 mL 0.2035 mL 0.4070 mL 1.0175 mL
    100 mM 0.0326 mL 0.1628 mL 0.3256 mL 0.8140 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Histamine phosphate Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Histamine51-74-1Histamine diphosphateAktp38 MAPKReactive Oxygen Species (ROS)Histamine ReceptorEndogenous MetaboliteApoptosisPKBProtein kinase Borganic nitrogenimmune responsesintestinal physiological functionsneurotransmitterInhibitorinhibitorinhibit

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