1. Immunology/Inflammation
  2. IFNAR
  3. IFN alpha-IFNAR-IN-1 hydrochloride

IFN alpha-IFNAR-IN-1 hydrochloride 

Cat. No.: HY-12836A Purity: 99.87%
COA Handling Instructions

IFN alpha-IFNAR-IN-1 hydrochloride is a nonpeptidic, low-molecular-weight inhibitor of the interaction between IFN-α and IFNAR. IFN alpha-IFNAR-IN-1 hydrochloride inhibits modified Vaccinia virus ankara (MVA)-induced IFN-α responses in murine bone-marrow-derived, Flt3- L-differentiated pDC cultures (BM-pDCs) (IC50=2-8 μM).

For research use only. We do not sell to patients.

IFN alpha-IFNAR-IN-1 hydrochloride Chemical Structure

IFN alpha-IFNAR-IN-1 hydrochloride Chemical Structure

CAS No. : 2070014-98-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 131 In-stock
Solution
10 mM * 1 mL in DMSO USD 131 In-stock
Solid
5 mg USD 119 In-stock
10 mg USD 158 In-stock
50 mg USD 422 In-stock
100 mg USD 739 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 20 publication(s) in Google Scholar

Other Forms of IFN alpha-IFNAR-IN-1 hydrochloride:

Top Publications Citing Use of Products

    IFN alpha-IFNAR-IN-1 hydrochloride purchased from MedChemExpress. Usage Cited in: Sci Rep. 2016 Jul 14;6:29665.  [Abstract]

    PAMs are first pretreated with IFN-alpha-IFNAR-IN-1 or mock pretreated with medium containing DMSO for 2 h. The cells are then infected with the HP-PRRSV virus at an MOI of 5 or mock-infected with DMEM for 12 h and 16 h, respectively. The cell lysates are collected and analyzed by western blot. The fold change of SAMHD1 protein is expressed as densitometric units of the band normalized to the β-actin level relative to the uninfected control. The error bar represents standard deviation from three
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    IFN alpha-IFNAR-IN-1 hydrochloride is a nonpeptidic, low-molecular-weight inhibitor of the interaction between IFN-α and IFNAR. IFN alpha-IFNAR-IN-1 hydrochloride inhibits modified Vaccinia virus ankara (MVA)-induced IFN-α responses in murine bone-marrow-derived, Flt3- L-differentiated pDC cultures (BM-pDCs) (IC50=2-8 μM)[1].

    In Vitro

    IFN alpha-IFNAR-IN-1 (compound 1) (18 μM; 24 h) specifically inhibits MVA-induced IFN-a responses by BMpDCs[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    315.86

    Formula

    C18H18ClNS

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CNCC1=CC=CC=C1SC2=C(C=CC=C3)C3=CC=C2.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (316.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 16.67 mg/mL (52.78 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1660 mL 15.8298 mL 31.6596 mL
    5 mM 0.6332 mL 3.1660 mL 6.3319 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.87%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.1660 mL 15.8298 mL 31.6596 mL 79.1490 mL
    5 mM 0.6332 mL 3.1660 mL 6.3319 mL 15.8298 mL
    10 mM 0.3166 mL 1.5830 mL 3.1660 mL 7.9149 mL
    15 mM 0.2111 mL 1.0553 mL 2.1106 mL 5.2766 mL
    20 mM 0.1583 mL 0.7915 mL 1.5830 mL 3.9574 mL
    25 mM 0.1266 mL 0.6332 mL 1.2664 mL 3.1660 mL
    30 mM 0.1055 mL 0.5277 mL 1.0553 mL 2.6383 mL
    40 mM 0.0791 mL 0.3957 mL 0.7915 mL 1.9787 mL
    50 mM 0.0633 mL 0.3166 mL 0.6332 mL 1.5830 mL
    DMSO 60 mM 0.0528 mL 0.2638 mL 0.5277 mL 1.3191 mL
    80 mM 0.0396 mL 0.1979 mL 0.3957 mL 0.9894 mL
    100 mM 0.0317 mL 0.1583 mL 0.3166 mL 0.7915 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    IFN alpha-IFNAR-IN-1 hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    IFN alpha-IFNAR-IN-1 hydrochloride
    Cat. No.:
    HY-12836A
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