2. Immunology/Inflammation
  3. IFNAR
  4. RIG012

RIG012 is a potent RIG-I inhibitor with an IC50 of 0.71 μM using the NADH-coupled ATPase assay. RIG012 inhibits IFN-β and ISG hRsad2 expression.

For research use only. We do not sell to patients.

CAS No. : 2642218-43-5

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Based on 5 publication(s) in Google Scholar

RIG012 Related Antibodies

Top Publications Citing Use of Products

    RIG012 purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Jun 17;99(6):e0066625.

    The concentration of RIG012 in the serum of non-pregnant female mice (n = 3) intraperitoneally injected once with 10 mg/kg was measured by mass spectrometer.

    RIG012 purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Jun 17;99(6):e0066625.

    RIG012 treatment did not protect against ZIKV-induced fetal demise in Ifnar1−/− mice. Time-mated Ifnar1−/− dams were treated with vehicle or 22.5 mg/kg RIG012 every 12 h from E6.5 to E14 and inoculated with 103 PFU of ZIKV-BRA on E7.5, and the rate of resorption was calculated at E14.5. Data are presented as the percentage of total fetuses (n = 61–100, from 7 to 10 dams per treatment group). Significance was determined by Fisher’s exact test.

    RIG012 purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Jun 17;99(6):e0066625.

    There were no significant differences in serum titers between vehicle/ZIKV-BRA and RIG012 (I.P, 22.5 mg/kg, every 12 h)/ZIKV-BRA (two-way ANOVA, P > 0.9999). Maternal viremia was assessed via plaque assay at 2, 4, and 7 days post-inoculation (dpi), and significance was determined by two-way ANOVA with Tukey’s multiple comparisons.

    RIG012 purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Jun 17;99(6):e0066625.

    There was no significant difference in infectious virus at the MFI between vehicle/ZIKV-BRA and RIG012 (I.P, 22.5 mg/kg, every 12 h)/ZIKV-BRA, and fetuses had undetectable levels of infectious ZIKV-BRA virus.Tissue titer was assessed via plaque assay of MFI and fetus samples harvested at E14.5, and significance was determined by two-way ANOVA with Sidak’s multiple comparisons. Significance annotations: ****, P ≤ 0.0001; ns, P > 0.05.

    RIG012 purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Jun 17;99(6):e0066625.

    Resorbed fetuses have significantly higher relative interferon-stimulated gene expression than their normal counterparts. ZIKV vRNA load measured by qRT-PCR (A), relative Ifnb (B), Rsad2 (C), and Mx1 (D) expression in the MFI and fetus were plotted against the outcome (resorbed vs normal fetal outcome) and separated by treatment (vehicle vs 22.5 mg/kg RIG012). Gene expression levels in E14.5 MFI and fetus samples were measured by qPCR and normalized to Hprt expression. The ddCT was calculated relative to samples harvested from PBS-inoculated controls. Data points represent individual samples. The mean with standard deviation is plotted. Two-way ANOVA with interaction (between treatment and outcome) and Tukey’s multiple comparisons were used to determine significance. Significance annotations: **, P ≤ 0.01; *, P ≤ 0.05; ns, P > 0.05.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    RIG012 is a potent RIG-I inhibitor with an IC50 of 0.71 μM using the NADH-coupled ATPase assay. RIG012 inhibits IFN-β and ISG hRsad2 expression[1].

    In Vitro

    RIG012 contains a strong chromophore that absorbs in the visible range (λmax of 470 nm). Upon binding to RIG-I, the 470 nm absorbance band of RIG012 undergoes a dramatic hyperchromic shift to higher intensity values[1].
    RIG012 (1, 1.25, 1.5, 2, 2.5, 3, 4 μM) has potent, dose-dependent inhibition of RIG-I signaling in HEK293T cells cotransfected with expression and reporter plasmids[1].
    RIG012 (1.25, 2.25, 4.5, 9, 18 μM) has a potent, dose-dependent inhibition of IFN-β and ISG hRsad2 expression in A549 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    RIG012 Related Antibodies
    Molecular Weight

    359.42

    Formula

    C23H21NO3
    CAS No.
    Appearance

    Solid

    Color

    Orange to red

    SMILES

    O=C1N=C2C3=C(C(C(OCC4=CC=CC=C4)=C2C1(CC)CC)=O)C=CC=C3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (92.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7823 mL 13.9113 mL 27.8226 mL
    5 mM 0.5565 mL 2.7823 mL 5.5645 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.33%

    SDS (251 KB)

    COA (255 KB) HNMR (263 KB) RP-HPLC (250 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.7823 mL 13.9113 mL 27.8226 mL 69.5565 mL
    5 mM 0.5565 mL 2.7823 mL 5.5645 mL 13.9113 mL
    10 mM 0.2782 mL 1.3911 mL 2.7823 mL 6.9557 mL
    15 mM 0.1855 mL 0.9274 mL 1.8548 mL 4.6371 mL
    20 mM 0.1391 mL 0.6956 mL 1.3911 mL 3.4778 mL
    25 mM 0.1113 mL 0.5565 mL 1.1129 mL 2.7823 mL
    30 mM 0.0927 mL 0.4637 mL 0.9274 mL 2.3186 mL
    40 mM 0.0696 mL 0.3478 mL 0.6956 mL 1.7389 mL
    50 mM 0.0556 mL 0.2782 mL 0.5565 mL 1.3911 mL
    60 mM 0.0464 mL 0.2319 mL 0.4637 mL 1.1593 mL
    80 mM 0.0348 mL 0.1739 mL 0.3478 mL 0.8695 mL
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    RIG012 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    RIG0122642218-43-5RIG 012RIG-012IFNARInterferon-α/β receptorInterferon-alpha/beta receptorRIG-IIFN-βISG hRsad2chromophoreInhibitorinhibitorinhibit

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    Cat. No.:
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