NucPE1
Based on 5 publication(s) in Google Scholar
NucPE1 (Nuc-H2O2 Probe) is a nuclear-localized fluorescent hydrogen peroxide that is specifically localized to cellular nuclei without appended targeting moieties.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 1404091-23-1
- Formula: C29H30BNO5
- Molecular Weight:483.36
-
Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-12289
DefactinibFAK Inhibitor -
HY-100965
Diphenyleneiodonium chlorideNOX Inhibitor -
HY-18950
GSK2795039NOX2 Inhibitor -
HY-N0532
MorronisidePyroptosis Inhibitor -
HY-100111
GLX351322NADPH Oxidase 4 Inhibitor -
HY-103469
Peroxy Orange 1H2O2 probe -
HY-D1159
HKOH-1rOH Indicator -
HY-P7205
IL-1 beta Protein, Human (CHO)
Publications Citing Use of MedChemExpress (MCE) NucPE1
More
Biological Activity
Description
In Vitro
NucPE1 features two major visible region absorptions (λabs = 468 nm, ε = 27,300/M/cm; λabs = 490 nm, ε = 26,000/M/cm) and a weak emission (λem = 530 nm, Φ = 0.117). Reaction of NucPE1 with H2O2 triggers a fluorescence increase upon its conversion to fluorophore NucPE1, which possesses one major absorption band at 505 nm (ε = 19,100/M/cm) with enhanced emission (λem = 530 nm, Φ = 0.626). NucPE1 selectively accumulates in the nuclei of a variety of mammalian cell lines as well as in whole model organisms like C. clegans, where it can respond to subcellular changes in H2O2 fluxes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
530
Excitation (Ex)
490
Chemical Information
-
CAS No. 1404091-23-1
-
Appearance Solid
-
Molecular Weight 483.36
-
Formula C29H30BNO5
-
Color White to pink
-
SMILES
O=C1OC2(C3=C(OC4=C2C=CC(B5OC(C)(C)C(C)(C)O5)=C4)C=C(NCC)C(C)=C3)C6=C1C=CC=C6
-
Synonyms
Nuc-H2O2 Probe
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (5)
-
Journal Impact Factor
-
Most Recent
-
Int J Biol Sci
NOX4-derived oxidative DNA damage impairs thyroid differentiation through an epigenetic mechanism in BRAF-mutated radioactive iodine refractory papillary thyroid cancer cells. [Abstract]2026 Jan 15;22(4):1674-1692. PMID: 41694580 -
Cell Biosci
2024 Jun 10;14(1):78. PMID: 38858714 -
FASEB J
Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter. [Abstract]2020 Sep;34(9):11474-11487. PMID: 32729950 -
-
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (51.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (280 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Stanicka J, et al. NADPH oxidase-generated hydrogen peroxide induces DNA damage in mutant FLT3-expressing leukemia cells. J Biol Chem. 2015 Apr 10;290(15):9348-61. [Content Brief]
[2]. Dickinson BC, et al. A nuclear-localized fluorescent hydrogen peroxide probe for monitoring sirtuin-mediated oxidative stress responses in vivo. Chem Biol. 2011 Aug 26;18(8):943-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0689 mL | 10.3443 mL | 20.6885 mL | 51.7213 mL |
| 5 mM | 0.4138 mL | 2.0689 mL | 4.1377 mL | 10.3443 mL | |
| 10 mM | 0.2069 mL | 1.0344 mL | 2.0689 mL | 5.1721 mL | |
| 15 mM | 0.1379 mL | 0.6896 mL | 1.3792 mL | 3.4481 mL | |
| 20 mM | 0.1034 mL | 0.5172 mL | 1.0344 mL | 2.5861 mL | |
| 25 mM | 0.0828 mL | 0.4138 mL | 0.8275 mL | 2.0689 mL | |
| 30 mM | 0.0690 mL | 0.3448 mL | 0.6896 mL | 1.7240 mL | |
| 40 mM | 0.0517 mL | 0.2586 mL | 0.5172 mL | 1.2930 mL | |
| 50 mM | 0.0414 mL | 0.2069 mL | 0.4138 mL | 1.0344 mL |
NucPE1 Related Classifications
HY-101859 Related Classifications
Powered by Bioz