Porcn-IN-1

Name: Porcn-IN-1
Brand: MedChemExpress
SKU: HY-111472
Rating: 4.5 (0 reviews)

Cat. No.: HY-111472 Purity: 99.57%: Data Sheet
COA

SDS
Handling Instructions
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Porcn-IN-1 is potent porcupine inhibitor with an IC₅₀ of 0.5±0.2 nM.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Porcn-IN-1 화학구조

CAS No. : 2036044-77-4

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

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Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

Porcn-IN-1 is potent porcupine inhibitor with an IC₅₀ of 0.5±0.2 nM.

IC₅₀ & Target

IC50: 0.5±0.2 nM (Porcupine inhibitor)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	0.5 nM Compound: 53	Inhibition of porcupine in mouse L Wnt3A cells co-cultured with HEK293 cells after 48 hrs by Super-top flash reporter gene assay Inhibition of porcupine in mouse L Wnt3A cells co-cultured with HEK293 cells after 48 hrs by Super-top flash reporter gene assay	[PMID: 27692509]

In Vitro

Porcupine is an enzyme that catalyses the addition of palmitoleate to a serine residue in Wnt proteins, a process which is required for the secretion of Wnt proteins. Porcupine-IN-1 is as potent as the clinical compound LGK974 in a cell based STF reporter gene assay. Porcn-IN-1 potently inhibits the secretion of Wnt3A, therefore is confirmed to be a porcupine inhibitor^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Porcn-IN-1 demonstrates moderate clearance under the treatment of human liver microsomes (57 mL/min/kg) and rat liver microsomes (24 mL/min/kg). It exhibits high clearance when treated with mouse microsomes (109 mL/min/kg)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

410.44

화학식

C₂₅H₁₉FN₄O

CAS No.

2036044-77-4

Appearance

Solid

Color

White to off-white

SMILES

FC1=C(C2=CC(C)=NC=C2)N=CC(CNC(C3=CC=C(C(C=CC=C4)=C4N5)C5=C3)=O)=C1

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

용액&용해도

In Vitro:

DMSO : ≥ 100 mg/mL (243.64 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4364 mL	12.1820 mL	24.3641 mL
5 mM	0.4873 mL	2.4364 mL	4.8728 mL
10 mM	0.2436 mL	1.2182 mL	2.4364 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 99.57%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (247 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Xu Z, et al. Design, synthesis, and evaluation of novel porcupine inhibitors featuring a fused 3-ring system based on the 'reversed' amide scaffold. [Content Brief]

Cell Assay ^[1]	HEK293T cells are transfected with pLinbin-Wnt3A plasmid or vehicle control. The HEK293T cells are then treated with or without compounds (Porcn-IN-1). Western Blot is used after 48 h to analyze both the cell lysis and culture medium^[1]. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
References	[1]. Xu Z, et al. Design, synthesis, and evaluation of novel porcupine inhibitors featuring a fused 3-ring system based on the 'reversed' amide scaffold. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4364 mL	12.1820 mL	24.3641 mL	60.9102 mL
	5 mM	0.4873 mL	2.4364 mL	4.8728 mL	12.1820 mL
	10 mM	0.2436 mL	1.2182 mL	2.4364 mL	6.0910 mL
	15 mM	0.1624 mL	0.8121 mL	1.6243 mL	4.0607 mL
	20 mM	0.1218 mL	0.6091 mL	1.2182 mL	3.0455 mL
	25 mM	0.0975 mL	0.4873 mL	0.9746 mL	2.4364 mL
	30 mM	0.0812 mL	0.4061 mL	0.8121 mL	2.0303 mL
	40 mM	0.0609 mL	0.3046 mL	0.6091 mL	1.5228 mL
	50 mM	0.0487 mL	0.2436 mL	0.4873 mL	1.2182 mL
	60 mM	0.0406 mL	0.2030 mL	0.4061 mL	1.0152 mL
	80 mM	0.0305 mL	0.1523 mL	0.3046 mL	0.7614 mL
	100 mM	0.0244 mL	0.1218 mL	0.2436 mL	0.6091 mL

Porcn-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Porcn-IN-12036044-77-4PorcupineInhibitorinhibitorinhibit

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Porcn-IN-1

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Porcn-IN-1 Related Antibodies

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Biological Activity

Protocol

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Porcn-IN-1 Related Antibodies

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농도 희석 계산기

Complete Stock Solution Preparation Table

Porcn-IN-1 Related Classifications

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