1. GPCR/G Protein
    Immunology/Inflammation
    Anti-infection
  2. CCR
    HIV
    CXCR

TAK-779 (Synonyms: Takeda 779)

Cat. No.: HY-13406 Purity: 99.73%
Handling Instructions

TAK-779 is a potent and selective nonpeptide antagonist of CCR5 and CXCR3, with a Ki of 1.1 nM for CCR5, and effectively and selectively inhibits R5 HIV-1, with EC50 and EC90 of 1.2 nM and 5.7 nM, respectively, in MAGI-CCR5 cells.

For research use only. We do not sell to patients.

TAK-779 Chemical Structure

TAK-779 Chemical Structure

CAS No. : 229005-80-5

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10 mM * 1 mL in DMSO USD 145 In-stock
Estimated Time of Arrival: December 31
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Estimated Time of Arrival: December 31
10 mg USD 222 In-stock
Estimated Time of Arrival: December 31
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

TAK-779 is a potent and selective nonpeptide antagonist of CCR5 and CXCR3, with a Ki of 1.1 nM for CCR5, and effectively and selectively inhibits R5 HIV-1, with EC50 and EC90 of 1.2 nM and 5.7 nM, respectively, in MAGI-CCR5 cells.

IC50 & Target[1][4]

MIP-1α-CCR5

1 nM (IC50, in CHO/CCR5 cells)

MIP-1β-CCR5

1 nM (IC50, in CHO/CCR5 cells)

RANTES-CCR5

1.4 nM (IC50, in CHO/CCR5 cells)

MCP-1-CCR2b

27 nM (IC50, in CHO/CCR5 cells)

R5 HIV-1 (Ba-L)

1.2 nM (EC50, in MAGI-CCR5 cells)

R5 HIV-1 (KK)

1.6 nM (EC50, in PBMCs)

R5 HIV-1 (HHA)

3.2 nM (EC50, in PBMCs)

R5 HIV-1 (CTV)

3.5 nM (EC50, in PBMCs)

R5 HIV-1 (Ba-L)

3.7 nM (EC50, in PBMCs)

R5 HIV-1 (Ba-L)

5.7 nM (EC90, in MAGI-CCR5 cells)

R5 HIV-1 (HHA)

7.5 nM (EC90, in PBMCs)

R5 HIV-1 (Ba-L)

12.8 nM (EC90, in PBMCs)

R5 HIV-1 (KK)

20.8 nM (EC90, in PBMCs)

R5 HIV-1 (CTV)

27 nM (EC90, in PBMCs)

mCXCR3

369 nM (IC50, in PBMCs)

In Vitro

TAK-779 is a potent and selective nonpeptide antagonist of CCR5, with a Ki of 1.1 nM, and effectively and selectively inhibits R5 HIV-1, with EC50 and EC90 of 1.2 nM and 5.7 nM, respectively, in MAGI-CCR5 cells. TAK-779 less potently blocks the binding of [125I]-monocyte chemotactic protein 1 to CCR2b in CHO/CCR2b cells, with an IC50 for CCR2b of 27 nM. TAK-779 also completely inhibits the binding of [125I]-RANTES to CHO/CCR5 cells with an IC50 of 1.4 nM. TAK-779 (20 nM) selectively inhibits CCR5-mediated Ca2+-signaling. In addition, TAK-779 shows no inhibition on X4 HIV-1 strains[1]. TAK-779 is an antagonist of CXCR3, and inhibits the migration of T cells but not T cell proliferation[2].

In Vivo

TAK-779 (10 mg/kg per day, s.c.) significantly prolongs the allograft survival of the rat intestinal transplantation model. TAK-779 also decreases the number of CD4+ as well as CD8+ T cells in spleen, blood and recipient mesenteric lymph nodes (MLN)[2]. TAK-779 (150 µg per mouse, s.c.) supppresses the development of experimental autoimmune encephalomyelitis (EAE) in myelin oligodendrocyte glycoprotein (MOG)-immunized C57BL/6 mice. TAK-779 decreases the infiltration of CXCR3 and CCR5 bearing leukocytes into the spinal cord. TAK-779 does not alter myelin oligodendrocyte glycoprotein (MOG)-specific immune responses or affect the potential of MOG-specific T cells to transfer experimental autoimmune encephalomyelitis (EAE)[3].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 25 mg/mL (47.07 mM)

H2O : 16.66 mg/mL (31.37 mM; Need ultrasonic and warming)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8828 mL 9.4139 mL 18.8278 mL
5 mM 0.3766 mL 1.8828 mL 3.7656 mL
10 mM 0.1883 mL 0.9414 mL 1.8828 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

The anti-HIV-1 activities of the test compounds (TAK-779, etc.) are based on the inhibition of virus-induced infectious focus formation in MAGI-CCR5 cells and the reduction of p24 antigen production in PBMCs. In brief, MAGI-CCR5 cells (1 × 104 cells per well) are cultured in a microtiter tray. After a 24-h incubation at 37°C, the culture supernatants are replaced with fresh culture media containing the virus (≈300 focus forming units per well) and various concentrations of the test compounds (TAK-779, etc.). After a 2-day incubation, the cells are fixed and stained with 5-bromo-4-chloro-3-indolyl-β-d-galactosidase. The number of infected (blue) cells is counted microscopically. For the PBMC assays, phytohemagglutinin-stimulated PBMCs (2.5 × 105 cells per 500 μl) are infected with HIV-1 in the presence of various concentrations of the test compounds (TAK-779, etc.). The amounts of the virus used for infection are, depending on the replicability of each strain, generally 1-10 ng of p24 per 2.5 × 105 cells. After an overnight incubation at 37°C, the cells are washed extensively to remove unadsorbed viral particles and are incubated further with culture media containing the same concentrations of the compounds as those used during viral adsorption. On day 6 after viral infection, the culture supernatants are collected and determined for their p24 antigen levels with a sandwich ELISA kit. The cytotoxicities of the compounds are evaluated in parallel with their antiviral activities. They are based on the viability and proliferation of mock-infected cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mice[3]
The mice are immunized with MOG and are treated s.c. with TAK-779 or vehicle. The mice (N= 10) are injected s.c. with 150 µg TAK-779 (dissolved in 5% mannitol solution) in a volume of 100 µL, once daily after MOG immunization. TAK-779 injection is started from day 0 after immunization and continued once daily for 22 days. The dose of 150 µg is determined based on the observations in prior experiments that the dose of 50 µg per mouse can not produce inhibition, and a dose of more than 100 µg per mouse is required to produce significant inhibition. The dose of 150 µg per mouse has also been used in other mouse experimental models, and approximately the same dose is used in allograft rejection and asthma models. As a control, an equal volume of PBS containing 5% mannitol is injected daily in the control mice (N= 10)[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

531.13

Formula

C₃₃H₃₉ClN₂O₂

CAS No.

229005-80-5

SMILES

C[N+](C)(CC1=CC=C(NC(C2=CC3=CC(C4=CC=C(C)C=C4)=CC=C3CCC2)=O)C=C1)C5CCOCC5.[Cl-]

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: 99.73%

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Product Name:
TAK-779
Cat. No.:
HY-13406
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TAK-779

Cat. No.: HY-13406