DNA Stain
There are several different stains that can be used to visualize and photograph DNA after separation by gel electrophoresis. Molecules of the dye adhere to DNA strands and fluoresce under UV light, showing where the bands are within the gel.
Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation/emission spectra.
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DNA Stain Related Products (91)
Related Products (91)
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Methyl Green zinc chloride
0 ImagesMethyl Green zinc chloride is a potent fluorescent dye. Methyl Green zinc chloride is a DNA stains of cells and electrophoretic gels. Methyl Green zinc chloride can be used as direct measuring of viability by both microscopy and flow cytometry, with peaks at 633 and 677 nm.
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DBCO-PEG4-TAMRA
0 ImagesCat. No.: HY-D1070CAS No.: 1895849-41-8DBCO-PEG4-TAMRA is a PEG-based TAMRA dye and contains a DBCO group, which enables Click Chemistry. The TAMRA dye is a dye widely used in oligonucleotide labeling and automated DNA sequencing applications. DBCO-PEG4-TAMRA is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
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6-TAMRA-SE
0 ImagesCat. No.: HY-D0049CAS No.: 150810-69-8Synonyms: 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing.
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Acridine Orange zinc chloride salt
0 ImagesCat. No.: HY-D0942CAS No.: 10127-02-3Synonyms: Euchrysine 3RXAcridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm).
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Cy5 maleimide (solution)
0 ImagesCat. No.: HY-DY1067CAS No.: 1437796-65-0Synonyms: Cyanine5 maleimide (solution)Cy5 maleimide (solution) is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
Solvent and concentration: DMSO: 10 mM
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Br-DAPI
0 ImagesBr-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells. Storage: Keep away from light.
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7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester
0 ImagesCat. No.: HY-D0115CAS No.: 134471-24-27-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is the amine-reactive succinimidyl ester of 7-Hydroxycoumarin-3-carboxylic acid. 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is a blue fluorescent dye for labeling proteins and nucleic acids.
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Cyanine 5 Tyramide methyl indole
0 ImagesCat. No.: HY-136247ACyanine 5 Tyramide (Tyramide-Cy5) methyl indole is a red fluorescent dye. Cyanine 5 Tyramide is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids. Storage: protect from light.
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6-Carboxy-X-rhodamine, succinimidyl ester
0 ImagesCat. No.: HY-D1086CAS No.: 216699-36-4Synonyms: 6-ROX, SE6-Carboxy-X-rhodamine, succinimidyl ester (6-ROX, SE) is a fluorescent dye for oligonucleotide labeling and automated DNA sequencing.
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Chlorotris(triphenylphosphine)copper
0 ImagesSynonyms: CuCl(TPP)3Chlorotris(triphenylphosphine)copper (CuCl(TPP)₃) is a DNA-targeted metal complex. Chlorotris(triphenylphosphine)copper involves non-covalent interactions (such as groove binding mode) through the copper(I) center to affect DNA function, showing inhibitory activity against bacteria, fungi, and tumor cells. Chlorotris(triphenylphosphine)copper is promising for research of antibacterial, antitumor, and antioxidant agents.
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Hexidium iodide
0 ImagesHexidium iodide, a fluorescent nucleic binding acid stain (excitation/emission ~ 518/600 nm), permeants to mammalian cells and selectively stains almost all gram-positive bacteria. Hexidium iodide can bind to the DNA of all bacteria after permeabilization by EDTA.
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Biotin-16-dCTP
0 ImagesCat. No.: HY-160841CAS No.: 2567941-52-8Biotin-16-dCTP is a biotinylated deoxycytidine triphosphate that serves as an important DNA labeling substrate. Biotin-16-dCTP can be enzymatically incorporated into the 3' end of DNA probes via terminal deoxynucleotidyl transferase, forming a 1-3 nucleotide-long tail to achieve biotinylation of the probes. Biotin-16-dCTP enhances chemiluminescent detection of low-abundance targets such as specific tRNA isoacceptors through Northern blotting. Biotin-16-dCTP can also replace conventional dCTP to be integrated into single-stranded DNA generated by asymmetric polymerase chain reaction, which is applicable for bioconjugation or pull-down assays. Repeated freeze-thaw cycles of Biotin-16-dCTP should be avoided to prevent degradation of its function for probe biotinylation.
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Hoechst 33258 analog 3
0 ImagesHoechst 33258 analog 3 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
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3,6-Dichlorotrimellitic acid
0 Images3,6-Dichlorotrimellitic acid is the key precursor that is used for preparing a variety of dichlorinated fluoresceins and rhodamines such as TET and HEX.
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HOE 32020
0 ImagesHOE 32020 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
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meta-iodoHoechst 33258
0 Imagesmeta-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
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Hoechst 33258 analog 5
0 ImagesHoechst 33258 analog 5 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
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Hoechst 33258 analog 2
0 ImagesHoechst 33258 analog 2 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
