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  3. DBCO-PEG4-TAMRA

DBCO-PEG4-TAMRA is a PEG-based TAMRA dye and contains a DBCO group, which enables Click Chemistry. The TAMRA dye is a dye widely used in oligonucleotide labeling and automated DNA sequencing applications. DBCO-PEG4-TAMRA is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

For research use only. We do not sell to patients.

DBCO-PEG4-TAMRA

DBCO-PEG4-TAMRA Chemical Structure

CAS No. : 1895849-41-8

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Description

DBCO-PEG4-TAMRA is a PEG-based TAMRA dye and contains a DBCO group, which enables Click Chemistry. The TAMRA dye is a dye widely used in oligonucleotide labeling and automated DNA sequencing applications. DBCO-PEG4-TAMRA is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

IC50 & Target

TARMA Dye

In Vitro

Click chemistry refers to a group of reactions that are fast, simple to use, easy to purify, versatile, regiospecific, and give high product yields. Click chemistry reaction is suitable for cell marker and low concentration reaction with low toxicity.
DBCO reacts with azide functionalized compounds or biomolecules without catalyst to form a stable triazole linkage, which is an ideal alternative to copper intolerant applications.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

936.06

Formula

C54H57N5O10

CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(C(NCCOCCOCCOCCOCCC(NCCC(N5C6=CC=CC=C6C#CC7=CC=CC=C7C5)=O)=O)=O)C=C4C([O-])=O)=C2C=C1)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (53.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0683 mL 5.3415 mL 10.6831 mL
5 mM 0.2137 mL 1.0683 mL 2.1366 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0683 mL 5.3415 mL 10.6831 mL 26.7077 mL
5 mM 0.2137 mL 1.0683 mL 2.1366 mL 5.3415 mL
10 mM 0.1068 mL 0.5342 mL 1.0683 mL 2.6708 mL
15 mM 0.0712 mL 0.3561 mL 0.7122 mL 1.7805 mL
20 mM 0.0534 mL 0.2671 mL 0.5342 mL 1.3354 mL
25 mM 0.0427 mL 0.2137 mL 0.4273 mL 1.0683 mL
30 mM 0.0356 mL 0.1781 mL 0.3561 mL 0.8903 mL
40 mM 0.0267 mL 0.1335 mL 0.2671 mL 0.6677 mL
50 mM 0.0214 mL 0.1068 mL 0.2137 mL 0.5342 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
DBCO-PEG4-TAMRA
Cat. No.:
HY-D1070
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