β-Casomorphin, bovine
Based on 1 Customer Validation
β-Casomorphin, bovine (β-Casomorphin-7 (bovine) ) is a opioid peptide with an IC50 of 14 μM in an Opioid receptors binding assay.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 72122-62-4
- Formula: C41H55N7O9
- Molecular Weight:789.92
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All Opioid Receptor Isoforms
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Biological Activity
IC50: 14 μM (Opioid receptor)[1]
β-Casomorphin, bovine (0.1 μM; 30 minutes-24 hours) stimulates the secretion of mucin in DHE Cells after 8 h of treatment[2].
β-Casomorphin, bovine (0.1 μM; 24 hours) induces an increase in rMuc2 and rMuc3 mRNA levels[2].
β-Casomorphin, bovine do not modify the expression of rMuc1, rMuc4, and rMuc5AC[2].
β-Casomorphin, bovine (0.1 μM; 24 hours) stimulates MUC5AC expression and mucin Secretion in HT29-MTX Cells (human)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
β-Casomorphin, bovine (7.5×10 6 mol/day/kg ; i.g.; for 30 days) alters the changes of SOD, GPx, T-AOC, MDA and H2O2 in the kidney of diabetic rats[3].
β-Casomorphin, bovine (7.5×10 6 mol/day/kg ; i.g.; for 30 days) reduces MDA (significantly) and H2O2 (non-significantly) level in the kidney compared to the diabetic rats[3].
β-Casomorphin, bovine (7.5×10 6 mol/day/kg ; i.g.; for 30 days) alleviates high glucose-induced decreasement in SOD and GPx activity, increasement in MDA contents in the NRK-52E cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley (SD) rats (200 g±10 g)[3]
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Dosage:7.5×10−6 mol/day/kg
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Administration:Oral gavage; for 30 days
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Result:Increased plasma insulin and decreased plasma glucagon of diabetic rats.
Chemical Information
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CAS No. 72122-62-4
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Appearance Solid
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Molecular Weight 789.92
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Formula C41H55N7O9
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Color White to off-white
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Synonyms
β-Casomorphin-7 (bovine); Bovine β-casomorphin-7
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Sequence
Tyr-Pro-Phe-Pro-Gly-Pro-Ile
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Sequence Shortening
YPFPGPI
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (126.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 50 mg/mL (63.30 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.16 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.16 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 25 mg/mL (31.65 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Brantl V, et al. Opioid activities of beta-casomorphins. Life Sci. 1981 Apr 27;28(17):1903-9. [Content Brief]
[2]. Zoghbi S, et al. β-casomorphin-7-7 regulates the secretion and expression of gastrointestinal mucins through a μ-opioid pathway. Am J Physiol Gastrointest Liver Physiol. 2006 Jun;290(6):G1105-13. [Content Brief]
[3]. Zhang W, et al. The protective effects of beta-casomorphin-7 against glucose -induced renal oxidative stress in vivo and vitro. PLoS One. 2013 May 3;8(5):e63472. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.2660 mL | 6.3298 mL | 12.6595 mL | 31.6488 mL |
| 5 mM | 0.2532 mL | 1.2660 mL | 2.5319 mL | 6.3298 mL | |
| 10 mM | 0.1266 mL | 0.6330 mL | 1.2660 mL | 3.1649 mL | |
| 15 mM | 0.0844 mL | 0.4220 mL | 0.8440 mL | 2.1099 mL | |
| 20 mM | 0.0633 mL | 0.3165 mL | 0.6330 mL | 1.5824 mL | |
| 25 mM | 0.0506 mL | 0.2532 mL | 0.5064 mL | 1.2660 mL | |
| 30 mM | 0.0422 mL | 0.2110 mL | 0.4220 mL | 1.0550 mL | |
| 40 mM | 0.0316 mL | 0.1582 mL | 0.3165 mL | 0.7912 mL | |
| 50 mM | 0.0253 mL | 0.1266 mL | 0.2532 mL | 0.6330 mL | |
| 60 mM | 0.0211 mL | 0.1055 mL | 0.2110 mL | 0.5275 mL | |
| DMSO | 80 mM | 0.0158 mL | 0.0791 mL | 0.1582 mL | 0.3956 mL |
| 100 mM | 0.0127 mL | 0.0633 mL | 0.1266 mL | 0.3165 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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