Aminopeptidase-IN-1

Name: Aminopeptidase-IN-1
Brand: MedChemExpress
SKU: HY-151518
Rating: 4.5 (1 reviews)

Cat. No.: HY-151518 Purity: 98.02%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Aminopeptidase-IN-1 (compound 16o) is a potent insulin-regulated aminopeptidase (IRAP) inhibitor with an K_i value of 7.7 μM. Aminopeptidase-IN-1 can be used tor research cognitive and memory impairments.

For research use only. We do not sell to patients.

Aminopeptidase-IN-1 Chemical Structure

CAS No. : 374102-08-6

Size	Price	Stock	Quantity
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

K_i: 7.7 μM (IRAP)^[1]

Molecular Weight

356.33

Formula

C₁₈H₁₆N₂O₆

CAS No.

374102-08-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(N)OC2=CC(O)=CC=C2C1C3=CC=C([N+]([O-])=O)C=C3)OCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (280.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8064 mL	14.0319 mL	28.0639 mL
5 mM	0.5613 mL	2.8064 mL	5.6128 mL
10 mM	0.2806 mL	1.4032 mL	2.8064 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.02%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (253 KB) HNMR (236 KB) LCMS (261 KB)

Handling Instructions (2659 KB)

References

[1]. Mountford SJ, et al. Synthesis, structure-activity relationships and brain uptake of a novel series of benzopyran inhibitors of insulin-regulated aminopeptidase. J Med Chem. 2014 Feb 27;57(4):1368-77. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8064 mL	14.0319 mL	28.0639 mL	70.1597 mL
	5 mM	0.5613 mL	2.8064 mL	5.6128 mL	14.0319 mL
	10 mM	0.2806 mL	1.4032 mL	2.8064 mL	7.0160 mL
	15 mM	0.1871 mL	0.9355 mL	1.8709 mL	4.6773 mL
	20 mM	0.1403 mL	0.7016 mL	1.4032 mL	3.5080 mL
	25 mM	0.1123 mL	0.5613 mL	1.1226 mL	2.8064 mL
	30 mM	0.0935 mL	0.4677 mL	0.9355 mL	2.3387 mL
	40 mM	0.0702 mL	0.3508 mL	0.7016 mL	1.7540 mL
	50 mM	0.0561 mL	0.2806 mL	0.5613 mL	1.4032 mL
	60 mM	0.0468 mL	0.2339 mL	0.4677 mL	1.1693 mL
	80 mM	0.0351 mL	0.1754 mL	0.3508 mL	0.8770 mL
	100 mM	0.0281 mL	0.1403 mL	0.2806 mL	0.7016 mL