3,4-Dichloroisocoumarin
Based on 1 Customer Validation
3,4-Dichloroisocoumarin is a potent serine-protease and SrLip inhibitor (Ki for SrLip: 26.6 μM). 3,4-Dichloroisocoumarin is opened by serine proteases and then undergoes acylation with the enzyme, thereby inhibiting protease activity. 3,4-Dichloroisocoumarin can induce DNA fragmentation and Apoptosis. 3,4-Dichloroisocoumarin can be used in the research of multiple fields such as tumors, cardiovascular disease and enzyme catalytic mechanisms.
For research use only. We do not sell to patients.
- Purity: 99.68%
- CAS No.: 51050-59-0
- Formula: C9H4Cl2O2
- Molecular Weight:215.03
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-L181
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HFF | IC50 |
12.5 μM
Compound: 3,4-DCI
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Inhibition of Toxoplasma gondii 2F1 invasion in HFF cells by red/green invasion assay
Inhibition of Toxoplasma gondii 2F1 invasion in HFF cells by red/green invasion assay
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[PMID: 17145790] |
3,4-Dichloroisocoumarin (1 mM, 4-6 h) induces oligonucleosomal DNA fragmentation in cell lines expressing high levels of protease activity (such as LAK, NK-92 cells, etc.)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Japanese white rabbits (weighing 1.9-2.5 kg) with myocardial ischemia-reperfusion model[5]
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Dosage:2 mg/kg (dissolved in DMSO)
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Administration:Intravenous injection (i.v.), once, 20 min before coronary occlusion
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Result:Reduced the percentage of DNA fragmentation in the infarcted area.
Attenuated the DNA ladder pattern.
Did not significantly reduce the myocardial infarct size.
Chemical Information
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CAS No. 51050-59-0
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Appearance Solid
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Molecular Weight 215.03
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Formula C9H4Cl2O2
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Color White to off-white
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SMILES
ClC1=C(OC(C2=C1C=CC=C2)=O)Cl
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Structure Classification
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Initial Source
pig
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (465.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Hameed A, et al. 3,4-Dichloroisocoumarin serine protease inhibitor induces DNA fragmentation and apoptosis in susceptible target cells. Proc Soc Exp Biol Med. 1998 Nov;219(2):132-7. [Content Brief]
[2]. Anees M, et al. Inhibition of a tumour protease with 3,4-dichloroisocoumarin, pentamidine-isethionate and guanidino derivatives. J Enzyme Inhib. 1994;8(3):213-21. [Content Brief]
[3]. Pereira ME, et al. 3,4-dichloroisocoumarin-induced activation of the degradation of beta-casein by the bovine pituitary multicatalytic proteinase complex. J Biol Chem. 1992 Apr 15;267(11):7949-55. [Content Brief]
[4]. Ašler IL, et al. Inhibition of extracellular lipase from Streptomyces rimosus with 3,4-dichloroisocoumarin. J Enzyme Inhib Med Chem. 2013 Oct;28(5):1094-104. [Content Brief]
[5]. Minatoguchi S, et al. Caspase-dependent and serine protease-dependent DNA fragmentation of myocytes in the ischemia-reperfused rabbit heart: these inhibitors do not reduce infarct size. Jpn Circ J. 2001 Oct;65(10):907-11. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.6505 mL | 23.2526 mL | 46.5051 mL | 116.2628 mL |
| 5 mM | 0.9301 mL | 4.6505 mL | 9.3010 mL | 23.2526 mL | |
| 10 mM | 0.4651 mL | 2.3253 mL | 4.6505 mL | 11.6263 mL | |
| 15 mM | 0.3100 mL | 1.5502 mL | 3.1003 mL | 7.7509 mL | |
| 20 mM | 0.2325 mL | 1.1626 mL | 2.3253 mL | 5.8131 mL | |
| 25 mM | 0.1860 mL | 0.9301 mL | 1.8602 mL | 4.6505 mL | |
| 30 mM | 0.1550 mL | 0.7751 mL | 1.5502 mL | 3.8754 mL | |
| 40 mM | 0.1163 mL | 0.5813 mL | 1.1626 mL | 2.9066 mL | |
| 50 mM | 0.0930 mL | 0.4651 mL | 0.9301 mL | 2.3253 mL | |
| 60 mM | 0.0775 mL | 0.3875 mL | 0.7751 mL | 1.9377 mL | |
| 80 mM | 0.0581 mL | 0.2907 mL | 0.5813 mL | 1.4533 mL | |
| 100 mM | 0.0465 mL | 0.2325 mL | 0.4651 mL | 1.1626 mL |
3,4-Dichloroisocoumarin Related Classifications
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