2. Metabolic Enzyme/Protease Anti-infection
  3. Ser/Thr Protease HPV
  4. TPCK

TPCK  (Synonyms: L-1-Tosylamido-2-phenylethyl chloromethyl ketone; L-TPCK)

Cat. No.: HY-124379 Purity: 98.49%
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TPCK (L-1-Tosylamido-2-phenylethyl chloromethyl ketone; L-TPCK) is a potent serine protease inhibitor that reacts with the retinoblastoma protein (RB)-binding core of HPV-18 E7 protein and abolish its RB-binding capability. TPCK is able to modify E7 protein in live keratinocytes following its addition to the culture medium.

For research use only. We do not sell to patients.

TPCK Chemical Structure

TPCK Chemical Structure

CAS No. : 402-71-1

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 25 In-stock
Solution
10 mM * 1 mL in DMSO USD 25 In-stock
Solid
5 mg USD 23 In-stock
10 mg USD 35 In-stock
25 mg USD 50 In-stock
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100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

TPCK Related Antibodies

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Description

TPCK (L-1-Tosylamido-2-phenylethyl chloromethyl ketone; L-TPCK) is a potent serine protease inhibitor that reacts with the retinoblastoma protein (RB)-binding core of HPV-18 E7 protein and abolish its RB-binding capability. TPCK is able to modify E7 protein in live keratinocytes following its addition to the culture medium[1].

Molecular Weight

351.85

Formula

C17H18ClNO3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=CC=C(C=C1)S(N[C@@H](CC2=CC=CC=C2)C(CCl)=O)(=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (284.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8421 mL 14.2106 mL 28.4212 mL
5 mM 0.5684 mL 2.8421 mL 5.6842 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8421 mL 14.2106 mL 28.4212 mL 71.0530 mL
5 mM 0.5684 mL 2.8421 mL 5.6842 mL 14.2106 mL
10 mM 0.2842 mL 1.4211 mL 2.8421 mL 7.1053 mL
15 mM 0.1895 mL 0.9474 mL 1.8947 mL 4.7369 mL
20 mM 0.1421 mL 0.7105 mL 1.4211 mL 3.5527 mL
25 mM 0.1137 mL 0.5684 mL 1.1368 mL 2.8421 mL
30 mM 0.0947 mL 0.4737 mL 0.9474 mL 2.3684 mL
40 mM 0.0711 mL 0.3553 mL 0.7105 mL 1.7763 mL
50 mM 0.0568 mL 0.2842 mL 0.5684 mL 1.4211 mL
60 mM 0.0474 mL 0.2368 mL 0.4737 mL 1.1842 mL
80 mM 0.0355 mL 0.1776 mL 0.3553 mL 0.8882 mL
100 mM 0.0284 mL 0.1421 mL 0.2842 mL 0.7105 mL
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TPCK Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TPCK402-71-1L-1-Tosylamido-2-phenylethyl chloromethyl ketone L-TPCKSer/Thr ProteaseHPVSerine proteasesSerine endopeptidasesThreonine proteasesHuman papillomavirusserine protease inhibitorretinoblastoma proteinHPV-18 E7 proteinInhibitorinhibitorinhibit

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