Anti-Mouse CCR2 Antibody (C2Mab-6)
Anti-Mouse CCR2 Antibody (C2Mab-6) is a monoclonal antibody targeting mouse CC chemokine receptor type 2 (mCCR2), with a KD value of 0.18-1.6 nM. Anti-Mouse CCR2 Antibody (C2Mab-6) exhibits high binding affinity to cells expressing mCCR2 and shows no reactivity to parental CHO-K1 cells. Anti-Mouse CCR2 Antibody (C2Mab-6) recognizes the amino acid region spanning positions 1-19 of mCCR2, and its key binding epitope includes Asp3, Asn4, Met6, Pro8, Gln9 and Phe10. Anti-Mouse CCR2 Antibody (C2Mab-6) can be used in leukemia-related research.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Rat IgG1 kappa
Mouse
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mCCR2 1.6 nM (Kd, CHO/mCCR2 cells) |
mCCR2 0.18 nM (Kd, L1210 cells) |
Anti-Mouse CCR2 Antibody (C2Mab-6) (0.01-10 μg/mL; 30 min) specifically binds to CHO/mCCR2 cells overexpressing mouse CCR2 and L1210 mouse leukemia cells expressing endogenous mouse CCR2 in a dose-dependent manner, but does not bind to parental CHO-K1 cells[1].
Anti-Mouse CCR2 Antibody (C2Mab-6) (0.0001-25 μg/mL) exhibits high binding affinity to CHO/mCCR2 cells overexpressing mouse CCR2 (KD = 1.6 nM) and L1210 mouse leukemia cells expressing endogenous mouse CCR2 (KD = 0.18 nM)[1].
Anti-Mouse CCR2 Antibody (C2Mab-6) (1 μg/mL; 30 min) specifically recognizes the N-terminal amino acid region 1-19 of mouse CCR2 via synthetic peptide ELISA[2].
Binding of Anti-Mouse CCR2 Antibody (C2Mab-6) (1 μg/mL; 30 min) to mouse CCR2 depends on the Asp3, Asn4, Met6, Pro8, Gln9, and Phe10 sites; ELISA assays show that the reactivity of this antibody disappears when the amino acids at these sites are substituted with alanine[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley (female, 6 weeks old)[1]
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Dosage:100 µg of each of three keyhole limpet hemocyanin-conjugated mCCR2 peptides
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Administration:i.p.; initial immunization, repeated three additional times; final booster 2 days before spleen harvest
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Result:Generated hybridomas, with 3 of 1916 wells producing CHO/mCCR2-reactive supernatants.
Established the C2Mab-6 antibody (rat IgG1, kappa) after cloning by limiting dilution.
P51683
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
Flow cytometry; ELISA
Chemical Information
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Tanaka T, et al. Development of a Novel Anti-Mouse CCR2 Monoclonal Antibody (C2Mab-6) by N-Terminal Peptide Immunization. Monoclon Antib Immunodiagn Immunother. 2022 Apr;41(2):80-86. [Content Brief]
[2]. Tanaka T, et al. Epitope Mapping of an Anti-Mouse CCR2 Monoclonal Antibody (CMab-6) Using Enzyme-Linked Immunosorbent Assay. Monoclonal antibodies in immunodiagnosis and immunotherapy. 2022 Dec;41(6):339-342. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CCR2 Antibody (C2Mab-6) Related Classifications
HY-P991745 Related Classifications
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