AFAP1 Antibody (YA8689)

Cat. No.: HY-P89005: Data Sheet User Guide for Antibodies
FAQs
Technical Support

AFAP1 Antibody (YA8689) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AFAP1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

AFAP1 Antibody (YA8689) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AFAP1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 80.5 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8N556

Immunogen

Human recombinant protein fragment corresponding to amino acids 100-386 of human AFAP1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells

Subcellular Localization：Cytoplasm, cytoskeleton, stress fiber

Expression：
Tissue_Specificity: Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma

Isoforms & Post-Translational Modification：Q8N556 has two isomers: Q8N556-1: 80725 Da (predicted); Q8N556-2: 89795 Da (predicted).
Phosphorylated on tyrosine residues by SRC

Subunit：Monomer and homomultimer

Synonyms

AFAP; AFAP-110; AFAP110

Documentation

User Guide for Antibodies (1077 KB)