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AF647-conjugated Goat Anti-Rabbit IgG H&L

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Based on 1 publication(s) in Google Scholar

AF647-conjugated Goat Anti-Rabbit IgG H&L is an AF647-conjugated,Goat-derived anti-Rabbit antibody. AF647-conjugated Goat Anti-Rabbit IgG H&L can binds to the IgG heavy and light chain of Rabbit antibodies.

For research use only. We do not sell to patients.
  • Host:

    Goat

  • Isotype:

    IgG

  • Application:

    ICC/IF, IF-Tissue, FC

  • Reactivity :

    Rabbit

  • Formulation:

    Supplied in PBS, pH7.4, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide

  • Conjugation:
    AF647
ALL ICC Others
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
  • AF647-conjugated Goat Anti-Rabbit IgG H&L Product Image
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Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
Dilution Ratio 1:100-1000 1:100-1000 1:1000-4000

Product Details

Description

AF647-conjugated Goat Anti-Rabbit IgG H&L is an AF647-conjugated,Goat-derived anti-Rabbit antibody. AF647-conjugated Goat Anti-Rabbit IgG H&L can binds to the IgG heavy and light chain of Rabbit antibodies.

  • Host Goat
  • Clonality Polyclonal
  • Species Reactivity
    Rabbit
Immunogen

Synthetic peptide corresponding to Rabbit IgG.

Purification

Affinity Purified

Conjugation

AF647

Modification

Unmodified

Target Specificity

IgG heavy and light chain

Antibody Form

Whole IgG Antibodies

Minimal Cross Reactivity

Human, Mouse

Isotype

IgG

RRID

AB_3102769

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH7.4, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Protect from light. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
    Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
    Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
    Please select the appropriate fluorescent dye based on your fluorescence imaging system. According to the species of the experimental sample you are studying and the host species of the primary antibody, choose the appropriate species of the secondary antibody.

Background

  • Function

    Secondary antibodies are antibodies that bind to primary antibodies in immunoassays. They do not directly recognize the target antigen but rather the Fc region of the primary antibody or the entire immunoglobulin (Ig). Secondary antibodies are typically conjugated to fluorescent groups (such as Alexa Fluor), enzymes (such as HRP and alkaline phosphatase), or proteins (such as biotin).

  • Research Field

    Immunology

AF647-conjugated Goat Anti-Rabbit IgG H&L Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • 1/16

    MedchemExpress Validation
     Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • 1/16

    MedchemExpress Validation
     Please select the appropriate fluorescent dye based on your fluorescence imaging system. According to the species of the experimental sample you are studying and the host species of the primary antibody, choose the appropriate species of the secondary antibody.

  • AF647-conjugated Goat Anti-Rabbit IgG H&L Product Image
    1/16
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
  • AF647-conjugated Goat Anti-Rabbit IgG H&L Product Image
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
    1/1
    MedchemExpress Validation
     Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
    1/1
    MedchemExpress Validation
     Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
    1/1
    MedchemExpress Validation
     Please select the appropriate fluorescent dye based on your fluorescence imaging system. According to the species of the experimental sample you are studying and the host species of the primary antibody, choose the appropriate species of the secondary antibody.
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for AF647-conjugated Goat Anti-Rabbit IgG H&L
  • AF647-conjugated Goat Anti-Rabbit IgG H&L Product Image
    MedchemExpress Validation

    Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • AF647-conjugated Goat Anti-Rabbit IgG H&L Product Image
    MedchemExpress Validation

    Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • AF647-conjugated Goat Anti-Rabbit IgG H&L Product Image
    MedchemExpress Validation

    Please select the appropriate fluorescent dye based on your fluorescence imaging system. According to the species of the experimental sample you are studying and the host species of the primary antibody, choose the appropriate species of the secondary antibody.

  • AF647-conjugated Goat Anti-Rabbit IgG H&L Product Image ICC
  • AF647-conjugated Goat Anti-Rabbit IgG H&L Product Image ICC
  • AF647-conjugated Goat Anti-Rabbit IgG H&L Product Image Others