Verbascoside
Based on 15 publication(s) in Google Scholar
Verbascoside is isolated from Acanthus mollis, acts as an ATP-competitive inhibitor of PKC, with an IC50 of 25 μM, and has antitumor, anti-inflammatory and antineuropathic pain activity.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.83%
- CAS. Nr.: 61276-17-3
- Formel: C29H36O15
- Molecular Weight:624.59
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Publications Citing Use of MedChemExpress (MCE) Verbascoside
More- Cell. 2021 Apr 1;184(7):1693-1705.e17. [Abstract]
- Dev Cell. 2025 Jun 23;60(12):1751-1767.e9. [Abstract]
- J Ethnopharmacol. 2024 May 6:331:118272. [Abstract]
- Int J Mol Sci. 2024 Jun 28;25(13):7112. [Abstract]
- Cell Cycle. 2024 Mar;23(5):537-554. [Abstract]
- FOOD BIOPROD PROCESS. 2023 Feb 11.
- Neurotox Res. 2026 Mar 19;44(2):12. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- Arch Physiol Biochem. 2025 Nov 24:1-19. [Abstract]
- Pancreatology. 2024 Aug;24(5):719-731. [Abstract]
- Arch Physiol Biochem. 2023 Dec;129(6):1177-1186. [Abstract]
- Cell Biochem Biophys. 2021 Jun;79(2):301-310. [Abstract]
- SSRN. 2023 Oct 31.
- Acta medica mediterr . 2023 Feb 28.
- Patent. US20210085739A1.
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IP
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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Histological Imaging/Staining
Biologische Aktivität
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PKC 25 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
5.81 μM
Compound: 2
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Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
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[PMID: 25981688] |
| Erythrocyte | IC50 |
28 μM
Compound: 6
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Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs
Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs
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[PMID: 12828473] |
| HCT-15 | IC50 |
2.73 μM
Compound: 2
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Cytotoxicity against human HCT15 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
Cytotoxicity against human HCT15 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
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[PMID: 25981688] |
| HEp-2 | EC50 |
0.26 μg/mL
Compound: 1, verbascoside
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Antiviral activity against RSV infected in human Hep2 cells assessed as virus-induced cytopathic effect administered 3 hrs postinfection
Antiviral activity against RSV infected in human Hep2 cells assessed as virus-induced cytopathic effect administered 3 hrs postinfection
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[PMID: 9599250] |
| HEp-2 | EC50 |
0.8 μg/mL
Compound: 1, verbascoside
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Antiviral activity against RSV long infected in human Hep2 cells assessed as virus-induced cytopathic effect after 3 days
Antiviral activity against RSV long infected in human Hep2 cells assessed as virus-induced cytopathic effect after 3 days
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[PMID: 9599250] |
| HEp-2 | EC50 |
76.9 μg/mL
Compound: 1, verbascoside
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Cytotoxicity against RSV long infected human Hep2 cells after 3 days
Cytotoxicity against RSV long infected human Hep2 cells after 3 days
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[PMID: 9599250] |
| HEp-2 | EC50 |
9.7 μg/mL
Compound: 1, verbascoside
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Antiviral activity against RSV A-2 infected in human Hep2 cells after 3 days by plaque neutralization assay
Antiviral activity against RSV A-2 infected in human Hep2 cells after 3 days by plaque neutralization assay
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[PMID: 9599250] |
| HEp-2 | IC50 |
108 μg/mL
Compound: 1, verbascoside
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Cytotoxicity against human Hep2 cells administered 3 hrs postinfection
Cytotoxicity against human Hep2 cells administered 3 hrs postinfection
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[PMID: 9599250] |
| HEp-2 | IC50 |
44 μg/mL
Compound: 1, verbascoside
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Cytotoxicity against mCMV RM-461 infected human Hep2 cells after 3 days
Cytotoxicity against mCMV RM-461 infected human Hep2 cells after 3 days
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[PMID: 9599250] |
| HEp-2 | IC50 |
71 μg/mL
Compound: 1, verbascoside
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Cytotoxicity against HSV2 infected human Hep2 cells after 3 days
Cytotoxicity against HSV2 infected human Hep2 cells after 3 days
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[PMID: 9599250] |
| HEp-2 | EC50 |
0.8 μg/mL
Compound: 4
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Antiviral activity against respiratory syncytial virus A2 in human Hep2 cells assessed as inhibition of virus-induced cytopathic effect
Antiviral activity against respiratory syncytial virus A2 in human Hep2 cells assessed as inhibition of virus-induced cytopathic effect
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[PMID: 9784173] |
| HEp-2 | IC50 |
76.9 μg/mL
Compound: 4
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Cytotoxicity against human Hep2 cells
Cytotoxicity against human Hep2 cells
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[PMID: 9784173] |
| Hepatocyte | IC50 |
4.6 μM
Compound: 5
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Hepatoprotective activity in ddY mouse primary cultured hepatocytes assessed as inhibition of D-galactosamine-induced cytotoxicity after 44 hrs by MTT assay
Hepatoprotective activity in ddY mouse primary cultured hepatocytes assessed as inhibition of D-galactosamine-induced cytotoxicity after 44 hrs by MTT assay
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[PMID: 20159656] |
| J774.A1 | EC50 |
4.9 x 10-4 M
Compound: 2
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Cytotoxicity against mouse J774A1 macrophages grown in monolayers by MTT assay
Cytotoxicity against mouse J774A1 macrophages grown in monolayers by MTT assay
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[PMID: 16309308] |
| L1210 | IC50 |
13 μM
Compound: 1
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Antiproliferative activity against mouse L1210 cells after 48 hrs by coulter counting analysis
Antiproliferative activity against mouse L1210 cells after 48 hrs by coulter counting analysis
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[PMID: 1812212] |
| L929 | IC50 |
17.8 μg/mL
Compound: 5
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Cytoprotective activity in mouse L929 cells assessed as inhibition of TNF-alpha-induced cytotoxicity after 44 hrs by MTT assay
Cytoprotective activity in mouse L929 cells assessed as inhibition of TNF-alpha-induced cytotoxicity after 44 hrs by MTT assay
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[PMID: 20159656] |
| P388 | ED50 |
2.6 μg/mL
Compound: 1
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Cytotoxicity against mouse P388 cells
Cytotoxicity against mouse P388 cells
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[PMID: 2380718] |
| Peritoneal macrophage | IC50 |
>100 μM
Compound: 5
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Inhibition of LPS-induced nitric oxide production in ddY mouse peritoneal macrophages
Inhibition of LPS-induced nitric oxide production in ddY mouse peritoneal macrophages
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[PMID: 20159656] |
| Peritoneal macrophage | IC50 |
>100 μM
Compound: 5
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Inhibition of LPS-induced TNFalpha production in ddY mouse peritoneal macrophages
Inhibition of LPS-induced TNFalpha production in ddY mouse peritoneal macrophages
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[PMID: 20159656] |
| SK-MEL-2 | IC50 |
8.52 μM
Compound: 2
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Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
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[PMID: 25981688] |
| SK-OV-3 | IC50 |
7.61 μM
Compound: 2
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Cytotoxicity against human SKOV3 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
Cytotoxicity against human SKOV3 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
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[PMID: 25981688] |
Verbascoside acts as an ATP-competitive inhibitor of PKC, with an IC50 of 25 μM. Verbascoside shows Kis of 22 and 28 μM with respect to ATP and histone, respectively. Verbascoside has potent antitumor activity against L-1210 cells, with an IC50 of 13 μM[1]. Verbascoside (5, 10 μM) suppresses 2,4-dinitrochlorobenzene (DNCB)-induced T cell costimulatory factors CD86 and CD54, proinflammatory cytokines, and NFκB pathway activation in THP-1 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 61276-17-3
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Appearance Solid
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Molecular Weight 624.59
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Formel C29H36O15
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Color White to yellow
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SMILES
O[C@@H]([C@H](OCCC1=CC(O)=C(O)C=C1)O2)[C@H]([C@@H]([C@H]2CO)OC(/C=C/C3=CC(O)=C(O)C=C3)=O)O[C@@](O[C@@H](C)[C@H](O)[C@H]4O)([H])[C@@H]4O
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Synonyms
Acteoside; Kusaginin; TJC160
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Structure Classification
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (15)
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Journal Impact Factor
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Most Recent
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Cell
2021 Apr 1;184(7):1693-1705.e17. PMID: 33770502 -
Dev Cell
STAT3-controlled CHI3L1/SPP1 positive feedback loop demonstrates the spatial heterogeneity and immune characteristics of glioblastoma. [Abstract]2025 Jun 23;60(12):1751-1767.e9. PMID: 39933531
Verbascoside purchased from MedChemExpress. Usage Cited in: Dev Cell. 2025 Jun 23;60(12):1751-1767.e9. [Abstract]
Verbascoside (VB; 10 μM; 24 h) did not interfere with CHI3L1-STAT3 interaction.
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J Ethnopharmacol
Radix Rehmanniae Praeparata promoted zebrafish fin regeneration through aryl hydrocarbon receptor-dependent autophagy. [Abstract]2024 May 6:331:118272. PMID: 38710459
Verbascoside purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 May 6:331:118272. [Abstract]
Bar charts showing the length of the regenerating fin of zebrafish exposure to different concentrations of Verbascoside (40, 80, 100, 150 mg/mL) ingredients at 2 dpa.
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Int J Mol Sci
Plantago major and Plantago lanceolata Exhibit Antioxidant and Borrelia burgdorferi Inhibiting Activities. [Abstract]2024 Jun 28;25(13):7112. PMID: 39000214 -
Cell Cycle
Osteopontin-driven partial epithelial-mesenchymal transition governs the development of middle ear cholesteatoma. [Abstract]2024 Mar;23(5):537-554. PMID: 38662954
Verbascoside purchased from MedChemExpress. Usage Cited in: Cell Cycle. 2024 Mar;23(5):537-554. [Abstract]
Verbascoside (CD44 inhibitor; 1.87 μg/ear; 0.2 mL administered into the middle ears) in Six weeks old male Sprague-Dawley (SD) rats could remarkably ameliorate these symptoms could ameliorate the development of middle ear cholesteatoma.
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Neurotox Res
Tubuloside B Alleviates Aβ25-35 Induced PC12 Cell Injury by Attenuating Pyroptosis, Apoptosis and Excessive Autophagy. [Abstract]2026 Mar 19;44(2):12. PMID: 41854817 -
Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
Arch Physiol Biochem
Verbascoside attenuates angiotensin-induced hypertension by inhibiting endoplasmic reticulum stress via the Nur77/GFPT2/CHOP pathway. [Abstract]2025 Nov 24:1-19. PMID: 41284022 -
Pancreatology
Expression of versican isoforms V0/V1 by pancreatic cancer associated fibroblasts increases fibroblast proliferation. [Abstract]2024 Aug;24(5):719-731. PMID: 38719756 -
Arch Physiol Biochem
Actoeside mitigated the renal proximal tubule cells damage triggered by high glucose through miR-766/VCAM1/NF-κB signalling pathway. [Abstract]2023 Dec;129(6):1177-1186. PMID: 34338087 -
Cell Biochem Biophys
Acteoside Presents Protective Effects on Cerebral Ischemia/reperfusion Injury Through Targeting CCL2, CXCL10, and ICAM1. [Abstract]2021 Jun;79(2):301-310. PMID: 33439460 -
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Verbascoside purchased from MedChemExpress. Usage Cited in: Acta medica mediterr . 2023 Feb 28.
Verbascoside (75, 150 mg/kg; po; daily for 4 weeks) 8 weeks Male C57BLKS/J db/db and db/+ mice at a dose of 150 mg/kg/d (db-150VB) significantly lowered the fasting blood glucose (FBG) level and albumin-to-creatinine ratio (ACR) compared to db/db mice.
Verbascoside purchased from MedChemExpress. Usage Cited in: Acta medica mediterr . 2023 Feb 28.
Verbascoside (VB; 75, 150 mg/kg; po; daily for 4 weeks) 8 weeks Male C57BLKS/J db/db and db/+ mice at a dose of 150 mg/kg/d (db-150VB) alleviated kidney pathologic damage, which was characterized by mesangial matrix expansion and glomerulosclerosis compared to diabetic mice.
Verbascoside purchased from MedChemExpress. Usage Cited in: Acta medica mediterr . 2023 Feb 28.
Verbascoside (VB; 75, 150 mg/kg; po; daily for 4 weeks) 8 weeks Male C57BLKS/J db/db and db/+ mice restored the decreased expression of nephrin and podocin.
Verbascoside purchased from MedChemExpress. Usage Cited in: Acta medica mediterr . 2023 Feb 28.
Verbascoside (VB; 75, 150 mg/kg; po; daily for 4 weeks) 8 weeks Male C57BLKS/J db/db and db/+ mice effectively reduced the IL-6, CXCL10, and MCP-1 protein expression in renal tissues, especially in the 150 mg/kg/d dose group.
Verbascoside purchased from MedChemExpress. Usage Cited in: Acta medica mediterr . 2023 Feb 28.
The cytotoxic effect of verbascoside (25, 50, 75, 100, 200, 300 and 400 μM) on MPC5 for 24 h determined by Cell Counting Kit-8 assay.
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Lösungsmittel & Löslichkeit
DMSO : ≥ 100 mg/mL (160.11 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (160.11 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protokoll
The lymphocytic mouse leukemia L1210 cells (ATCC, CCL 219) are plated sparsely at 104 cells per well in 24-well cluster plates in Dulbecco’s modified Eagle medium containing 10% fetal calf serum, 4 mM glutamine, 100 U/mL penicillin, 100 µg/mL streptomycin sulfate, and Verbascoside (solubilized in DMSO). After a 2-day incubation period at 37°C in a humidified atmosphere (5% CO2 in air), growth is monitored by counting cell numbers in a Coulter-counter. IC50 values are calculated on the basis of the linear regression lines established for each compound tested[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Rats[2]
To induce atopic dermatitis (AD)-like symptoms, 2,4-dinitrochlorobenzene (DNCB) is used. Briefly, the dorsal hair of the mice is removed using an electronic clipper 2 days before DNCB treatment. An application of 200 µL of 1% DNCB (in acetone:olive oil = 4:1) is made to the shaved dorsal skin for sensitization. The repeated challenge is performed on the same site with 0.2% DNCB once every 3 days for about 2 weeks. The mice are divided into 4 groups (n = 6 per group): (1) vehicle-treated controls, (2) DNCB-treated only, (3) 1% Verbascoside (in acetone:olive oil 4:1)-treated only, and (4) DNCB + 1% Verbascoside-treated group[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (282 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Herbert JM, et al. Verbascoside isolated from Lantana camara, an inhibitor of protein kinase C. J Nat Prod. 1991 Nov-Dec;54(6):1595-600. [Content Brief]
[2]. Li Y, et al. Verbascoside Alleviates Atopic Dermatitis-Like Symptoms in Mice via Its Potent Anti-Inflammatory Effect. Int Arch Allergy Immunol. 2018;175(4):220-230. [Content Brief]
[3]. Amin B, et al. The Effect of Verbascoside in Neuropathic Pain Induced by Chronic Constriction Injury in Rats. Phytother Res. 2016 Jan;30(1):128-35. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.6011 mL | 8.0053 mL | 16.0105 mL | 40.0263 mL |
| 5 mM | 0.3202 mL | 1.6011 mL | 3.2021 mL | 8.0053 mL | |
| 10 mM | 0.1601 mL | 0.8005 mL | 1.6011 mL | 4.0026 mL | |
| 15 mM | 0.1067 mL | 0.5337 mL | 1.0674 mL | 2.6684 mL | |
| 20 mM | 0.0801 mL | 0.4003 mL | 0.8005 mL | 2.0013 mL | |
| 25 mM | 0.0640 mL | 0.3202 mL | 0.6404 mL | 1.6011 mL | |
| 30 mM | 0.0534 mL | 0.2668 mL | 0.5337 mL | 1.3342 mL | |
| 40 mM | 0.0400 mL | 0.2001 mL | 0.4003 mL | 1.0007 mL | |
| 50 mM | 0.0320 mL | 0.1601 mL | 0.3202 mL | 0.8005 mL | |
| 60 mM | 0.0267 mL | 0.1334 mL | 0.2668 mL | 0.6671 mL | |
| 80 mM | 0.0200 mL | 0.1001 mL | 0.2001 mL | 0.5003 mL | |
| 100 mM | 0.0160 mL | 0.0801 mL | 0.1601 mL | 0.4003 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.