Salviolone
Based on 1 publication(s) in Google Scholar
Salviolone is a natural diterpenoid derivative that can against melanoma cells. Salviolone exhibits a pleiotropic effect against melanoma by hampering cell cycle progression, STAT3 signaling, and malignant phenotype of A375 melanoma cells.
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- Reinheit : 99.32%
- CAS. Nr.: 119400-86-1
- Formel: C18H20O2
- Molecular Weight:268.35
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Speicherung:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biologische Aktivität
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Publications Citing Use of MedChemExpress (MCE) Salviolone
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Biologische Aktivität
Beschreibung
In Vitro
Salviolone (5-60 μM; 72 hours) reduces cell viability in the A375 and MeWo melanoma cell lines with EC50 values of 17 µM and 22 µM, respectively. Salviolone does not affect the growth of normal melanocytes[1].
Salviolone (20 μM; 48-72 hours) strongly reduces pRb, pCdk2, and cyclin A2, Tyr705-STAT3 phosphorylation expression levels in A375 cells. Salviolone also strongly increases the P21 and P53 protein expression level. Salviolone induces sustained activation of the phosphorylation of ERK1/2 and Akt[1].
Salviolone (10-20 μM) inhibits MMP2 gelatinase activity in the A375 melanoma cell line[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A375, MeWo melanoma cells, and NHEM cells
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Concentration:5 μM, 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM
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Incubation Time:72 hours
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Result:Impaired the viability of melanoma cells without affecting the growth of normal melanocytes.
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Cell Line:A375 cells
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Concentration:20 μM
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Incubation Time:48 hours, 72 hours
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Result:Reduced the expression of the active forms of Cdk2 (pCdk2) and cyclin A2, and the phosphorylation of Rb.
Chemical Information
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CAS. Nr. 119400-86-1
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Appearance Solid
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Molecular Weight 268.35
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Formel C18H20O2
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Color Light yellow to yellow
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SMILES
O=C1C(C)=CC2=CC=C3C(C)(C)CCCC3=C2C=C1O
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Multi-Targeting Carnosic Acid Kills Drug-Resistant Helicobacter pylori With Narrow-Spectrum Activity. [Abstract]2026 Jun 11:e76080. PMID: 42272345
Protokoll
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Flow cytometric DNA-content cell-cycle staining
Flow cytometric DNA-content cell-cycle staining measures the fluorescence intensity of DNA-bound fluorochromes in single cells or nuclei to estimate DNA content distributions, allowing assignment of populations to G0/G1, S, and G2/M phases by DNA histogram deconvolution. Propidium iodide (PI) intercalates into DNA, and PI fluorescence is proportional to cellular DNA content when staining is performed under conditions that make DNA accessible and minimize non-DNA signal. Cells with G2/M DNA content are expected to show approximately twice the fluorescence intensity of G0/G1 cells, while S-phase cells occupy intermediate fluorescence values. PI-based DNA-content analysis can also detect cells with fractional DNA content, often reported as sub-G1, when DNA fragmentation and extraction during staining reduce retained DNA signal in apoptotic cells. DAPI is an alternative DNA fluorochrome for univariate DNA-content analysis, while bivariate approaches combining DNA content with proliferation
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BrdU Incorporation Assay
Bromodeoxyuridine (BrdU) incorporation assay is based on the principle that BrdU, a thymidine analog, is incorporated into newly synthesized DNA during the S phase of the cell cycle, thereby serving as a marker of DNA replication and cellular proliferation. Incorporated BrdU can be detected using anti-BrdU antibodies following DNA denaturation, enabling visualization or quantification of proliferating cells through immunochemical detection methods such as immunofluorescence or immunohistochemistry.
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Protocol for Cell Cycle
Cell-cycle analysis by flow cytometry measures DNA content in single cells to estimate the fraction of cells in G0/G1, S, and G2/M phases. Propidium iodide intercalates into DNA, and after RNA removal with RNase, fluorescence intensity reflects cellular DNA content: 2N cells are assigned to G0/G1, cells between 2N and 4N to S phase, and 4N cells to G2/M. DNA-content analysis alone cannot reliably separate G0 from G1 or G2 from M. Ki-67 can distinguish quiescent G0 cells from cycling cells, EdU or BrdU incorporation marks active DNA synthesis in S phase, and phospho-histone H3 staining identifies mitotic cells within the 4N population.
Reinheit & Dokumentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Valentina Zanrè, et al. Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation. Int J Mol Sci. 2022 Jan 20;23(3):1121. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
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