Cat. No.: HY-N0859

Schisanhenol

(Synonyms: Schizanhenol; Gomisin-K3)

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Schisanhenol (Schizanhenol), a lignan, is an orally active antioxidant. Schisanhenol reduces AChE activity, increases SIRT1 and PGC-1α expression, and decreases phosphorylated Tau (Ser 396) levels. Schisanhenol increases SOD and glutathione peroxidase activity, decreases malondialdehyde (MDA) content, and inhibits UGT2B7 activitY. Schisanhenol attenuates ox-LDL-induced apoptosis, intracellular reactive oxygen species generation, and cytotoxicity in endothelial cells. Schisanhenol inhibits LDL oxidation, brain mitochondrial and membrane peroxidative damage, and brain mitochondrial swelling and disintegration. Schisanhenol can be used for the research of Alzheimer’s disease, atherosclerosis, brain ischemia, and age-related brain deterioration.

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Pureté: 99.99%
CAS No.: 69363-14-0
Formule: C₂₃H₃₀O₆
Masse moléculaire:402.48

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Schisanhenol Chemical Structure

CAS No.: 69363-14-0

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

AChE

SIRT1

Voir tous les produits spécifiques à Isoform Cholinesterase (ChE) :

AChE
BChE

Voir tous les produits spécifiques à Isoform Sirtuin :

Cellular Effect

Cell Line	Type	Value	Description	References
H9	EC₅₀	5.7 μM Compound: 6	Antiviral activity against HIV1 3B in H9 cells after 4 days by p24-antigen ELISA Antiviral activity against HIV1 3B in H9 cells after 4 days by p24-antigen ELISA	[PMID: 17190445]
H9	IC₅₀	42 μM Compound: 6	Cytotoxicity against human H9 cells after 4 days Cytotoxicity against human H9 cells after 4 days	[PMID: 17190445]

In Vitro

Schisanhenol attenuates ox-LDL-induced apoptosis and reactive oxygen species generation in cultured bovine aorta endothelial cells^[1].
Schisanhenol scavenges oxygen radicals in cultured human neutrophils^[1].
Schisanhenol protects cultured mouse spleen lymphocytes, isolated rat cerebral mitochondria and synaptosomes from oxygen free radical-induced injury^[1].
Schisanhenol (10-50 μM; 1 h pre-incubation, then 24 h co-incubation with ox-LDL) protects bovine aorta endothelial cells against ox-LDL-induced cytotoxicity in a dose-dependent manner, with 50 μM producing the strongest effect by preserving cell morphology, increasing cell viability, and reducing LDH leakage^[3].
Schisanhenol (10-50 μM; 24 h co-incubation with ox-LDL) inhibits ox-LDL-induced apoptosis in bovine aorta endothelial cells in a dose-dependent manner, with 10 μM producing a stronger reduction in sub-G1 apoptotic cells^[3].
Schisanhenol (10-50 μM; 30 min pre-incubation with DCFH-DA, then 15 min stimulation with ox-LDL) inhibits ox-LDL-induced intracellular reactive oxygen species generation in bovine aorta endothelial cells, with 10 μM returning ROS levels to normal control values^[3].
Schisanhenol (1-100 μM; 30 min) inhibits Fe²⁺-cysteine-induced lipid peroxidation and ATPase activity loss in 8-month-old rat brain membrane fractions in vitro in a dose-dependent manner^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[3]

Cell Line:

Bovine aorta endothelial cells (BAECs)
Concentration:

5-50 μM
Incubation Time:

1 h pre-incubation, then 24 h co-incubation with ox-LDL
Result:

Increased cell survival.
Reduced LDH leakage.
Preserved normal BAEC morphology.
Did not significantly increase cell viability or reduce LDH leakage at 5 μM.

Apoptosis Analysis^[3]

Cell Line:

Bovine aorta endothelial cells (BAECs)
Concentration:

10 μM, 50 μM
Incubation Time:

24 h co-incubation with ox-LDL
Result:

Reduced ox-LDL-induced chromatin condensation at 10 and 50 μM.
Diminished the intensity of typical internucleosomal DNA laddering at 10 and 50 μM.
Decreased the percentage of apoptotic cells in the sub-G1 phase to 33.9% at 50 μM.
Decreased the percentage of apoptotic cells in the sub-G1 phase to 23.8% at 10 μM.

In Vivo

Schisanhenol (10-100 mg/kg; i.p.; daily; 7 days) attenuates Scopolamine (HY-N0296)-induced cognitive impairment, reduces hippocampal AChE activity and oxidative damage, and modulates the SIRT1-PGC-1α-Tau signaling pathway in male Kunming mice^[1].
Schisanhenol (150 mg/kg; p.o.) significantly increases brain cytosol glutathione-peroxidase activity in mice subjected to brain ischemia-reperfusion, with a measured activity of 0.898 nmol/mg/5 min^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Kunming mice (male, 20-30 g) injected with Scopolamine^[1]
Dosage:

10 mg/kg; 30 mg/kg; 100 mg/kg
Administration:

i.p.; daily; 7 days
Result:

Significantly reduced escape latency in the Morris water maze.
Significantly increased time spent in the target quadrant during the probe test.
Significantly reduced acetylcholinesterase (AChE) and malondialdehyde (MDA) activity in hippocampal tissue.
Significantly increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) activity in hippocampal tissue.
Significantly increased SIRT1 and PGC-1α protein levels in hippocampal tissue.
Significantly reduced phosphorylated Tau (Ser396) protein levels in hippocampal tissue.

Chemical Information

CAS No. 69363-14-0
Appearance Solid
Masse moléculaire 402.48
Formule C₂₃H₃₀O₆
Color White to off-white
SMILES

COC(C(OC)=C(OC)C=C1C[C@H](C)[C@H](C)C2)=C1C3=C2C=C(OC)C(OC)=C3O
Synonyms

Schizanhenol; Gomisin-K3
Structure Classification
- Phenylpropanoids
- Lignans
- Phenols
- Monophenols
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvant et solubilité

In Vitro:

DMSO : 250 mg/mL (621.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4846 mL

12.4230 mL

24.8460 mL

5 mM

0.4969 mL

2.4846 mL

4.9692 mL

10 mM

0.2485 mL

1.2423 mL

2.4846 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 6.25 mg/mL (15.53 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 6.25 mg/mL (15.53 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (287 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Han Y, et al. Schisanhenol improves learning and memory in scopolamine-treated mice by reducing acetylcholinesterase activity and attenuating oxidative damage through SIRT1-PGC-1α-Tau signaling pathway. Int J Neurosci. 2019;129(2):110-118. [Content Brief]

[2]. Song JH, et al. Inhibition of UDP-Glucuronosyltransferases (UGTs) Activity by constituents of Schisandra chinensis. Phytother Res. 2015;29(10):1658-1664. [Content Brief]

[3]. Yu LH, et al. Schisanhenol attenuated ox-LDL-induced apoptosis and reactive oxygen species generation in bovine aorta endothelial cells in vitro. J Asian Nat Prod Res. 2008;10(7-8):799-806. [Content Brief]

[4]. Xue JY, et al. Antioxidant activity of two dibenzocyclooctene lignans on the aged and ischemic brain in rats. Free Radic Biol Med. 1992;12(2):127-135. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4846 mL	12.4230 mL	24.8460 mL	62.1149 mL
	5 mM	0.4969 mL	2.4846 mL	4.9692 mL	12.4230 mL
	10 mM	0.2485 mL	1.2423 mL	2.4846 mL	6.2115 mL
	15 mM	0.1656 mL	0.8282 mL	1.6564 mL	4.1410 mL
	20 mM	0.1242 mL	0.6211 mL	1.2423 mL	3.1057 mL
	25 mM	0.0994 mL	0.4969 mL	0.9938 mL	2.4846 mL
	30 mM	0.0828 mL	0.4141 mL	0.8282 mL	2.0705 mL
	40 mM	0.0621 mL	0.3106 mL	0.6211 mL	1.5529 mL
	50 mM	0.0497 mL	0.2485 mL	0.4969 mL	1.2423 mL
	60 mM	0.0414 mL	0.2070 mL	0.4141 mL	1.0352 mL
	80 mM	0.0311 mL	0.1553 mL	0.3106 mL	0.7764 mL
	100 mM	0.0248 mL	0.1242 mL	0.2485 mL	0.6211 mL

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Schisanhenol

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Voir tous les produits spécifiques à Isoform Cholinesterase (ChE)

Voir tous les produits spécifiques à Isoform Sirtuin

Activité biologique

Chemical Information

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Schisanhenol Related Classifications

HY-N0859 Related Classifications