Cat. No.: HY-N0271

Echinocystic acid

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Echinocystic acid is a natural pentacyclic triterpene with potent antioxidant, anti-inflammatory and analgesic activities.

For research use only. We do not sell to patients.

Purity: 99.00%
CAS No.: 510-30-5
Formula: C₃₀H₄₈O₄
Molecular Weight:472.70

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Echinocystic acid Chemical Structure

CAS No.: 510-30-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Echinocystic acid is a natural pentacyclic triterpene with potent antioxidant, anti-inflammatory and analgesic activities^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
HT-29	EC₅₀	>10 μM Compound: 9	Anti-necroptotic activity in human HT-29 cells assessed as inhibition of TNFalpha/SM-164/Z-VAD-fmk (TSZ)-induced necroptosis by measuring increase in cell viability measured after 12 hrs by celltiter-glo luminescent cell viability assay Anti-necroptotic activity in human HT-29 cells assessed as inhibition of TNFalpha/SM-164/Z-VAD-fmk (TSZ)-induced necroptosis by measuring increase in cell viability measured after 12 hrs by celltiter-glo luminescent cell viability assay	[PMID: 33248849]
MDCK	CC₅₀	15.41 μM Compound: EA	Cytotoxicity against MDCK cells measured after 48 hrs by alamar blue assay Cytotoxicity against MDCK cells measured after 48 hrs by alamar blue assay	[PMID: 27565552]
SK-OV-3	IC₅₀	21.47 μM Compound: Echinocystic acid	Reversal of platinum resistance in cisplatin-resistant human SK-OV-3 cells assessed as reduction in cisplatin IC50 after 48 hrs in presence of cisplatin by SRB assay (Rvb = 28.23 microM) Reversal of platinum resistance in cisplatin-resistant human SK-OV-3 cells assessed as reduction in cisplatin IC50 after 48 hrs in presence of cisplatin by SRB assay (Rvb = 28.23 microM)	[PMID: 35500202]
SK-OV-3	IC₅₀	96.45 μM Compound: Echinocystic acid	Cytotoxicity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 48 hrs by SRB assay Cytotoxicity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 48 hrs by SRB assay	[PMID: 35500202]

In Vitro

Echinocystic acid (2.5-10 μM; pretreated 1 h) inhibits IL-1β-induced NF-κB and MAPK activation^[1].
Echinocystic acid suppresses IL-1β-induced MMP-13, NO, and PGE2 production in a dose-dependent manner. IL-1β up-regulated the expression of COX-2 and iNOS, and the increase is inhibited by Echinocystic acid^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

Chondrocytes
Concentration:

2.5 μM, 5 μM, 10 μM
Incubation Time:

Pretreated 1 h and then stimulated with IL-1β for 30 min.
Result:

IL-1β-induced NF-κB and MAPK activation were inhibited.

In Vivo

Echinocystic acid (5 mg/kg intragastrically daily for 5 days) shows anti-depression activities in the mouse model of reserpine-induced pain-depression dyad^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male adult C57BL/6 mice (8-10 week old; 18 to 22 g) injected weith Reserpine^[2]
Dosage:

5 mg/kg
Administration:

Intragastrically; daily for 5 days
Result:

Attenuated reserpine-induced pain/depression dyad partially through regulating the biogenic amines levels and GluN2B receptors in the hippocampus.

Chemical Information

CAS No. 510-30-5
Appearance Solid
Molecular Weight 472.70
Formula C₃₀H₄₈O₄
Color White to off-white
SMILES

CC1(C)CC[C@]2(C(O)=O)[C@H](O)C[C@@]3(C)[C@]4(C)CC[C@@]5([H])C(C)(C)[C@@H](O)CC[C@]5(C)[C@@]4([H])CC=C3[C@]2([H])C1
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

All Histological Imaging/StainingELISART-PCRIFWB

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Journal Impact Factor

J Ethnopharmacol

Xihuang pill suppressed primary liver cancer growth by downregulation of AFP and YAP signaling. [Abstract]

2025 May 28:348:119891. PMID: 40294663
Immunol Res

Echinocystic acid ameliorates ischemic acute kidney injury in neonatal rats by attenuating ferroptosis via the Nrf2/GPX4 pathway. [Abstract]

2025 Mar 11;73(1):58. PMID: 40067558

Echinocystic acid purchased from MedChemExpress. Usage Cited in: Immunol Res. 2025 Mar 11;73(1):58. [Abstract]

H&E staining was performed to assess kidney tissue pathology treated with Echinocystic acid (EA) (20, 40 mg/kg, i.p.).

Echinocystic acid purchased from MedChemExpress. Usage Cited in: Immunol Res. 2025 Mar 11;73(1):58. [Abstract]

IL-6, IL-1β, and TNF-α concentrations in kidney tissues were quantifed using ELISA kits treated with Echinocystic acid (EA) (20, 40 mg/kg, i.p.).

Echinocystic acid purchased from MedChemExpress. Usage Cited in: Immunol Res. 2025 Mar 11;73(1):58. [Abstract]

Relative mRNA expression levels of IL-6, IL-1β, and TNF-α were assessed by RT-qPCR treated with Echinocystic acid (EA) (20, 40 mg/kg, i.p.).

Echinocystic acid purchased from MedChemExpress. Usage Cited in: Immunol Res. 2025 Mar 11;73(1):58. [Abstract]

The F4/80 expression in kidney tissues was determined by immunofuorescence treated with Echinocystic acid (EA) (20, 40 mg/kg, i.p.).

Echinocystic acid purchased from MedChemExpress. Usage Cited in: Immunol Res. 2025 Mar 11;73(1):58. [Abstract]

The expression of cleaved-PARP and cleavedcaspase 3 proteins in kidney tissues was evaluated by Western blotting treated with Echinocystic acid (EA) (20, 40 mg/kg, i.p.).
SSRN

2024 Feb 23.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (105.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1155 mL

10.5775 mL

21.1551 mL

5 mM

0.4231 mL

2.1155 mL

4.2310 mL

10 mM

0.2116 mL

1.0578 mL

2.1155 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.75 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ma Z et al. Echinocystic Acid Inhibits IL-1β-Induced COX-2 and iNOS Expression in Human Osteoarthritis Chondrocytes. Inflammation. 2016 Apr;39(2):543-9. [Content Brief]

[2]. Li S et al. Echinocystic acid reduces reserpine-induced pain/depression dyad in mice. Metab Brain Dis. 2016 Apr;31(2):455-63. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1155 mL	10.5775 mL	21.1551 mL	52.8877 mL
	5 mM	0.4231 mL	2.1155 mL	4.2310 mL	10.5775 mL
	10 mM	0.2116 mL	1.0578 mL	2.1155 mL	5.2888 mL
	15 mM	0.1410 mL	0.7052 mL	1.4103 mL	3.5258 mL
	20 mM	0.1058 mL	0.5289 mL	1.0578 mL	2.6444 mL
	25 mM	0.0846 mL	0.4231 mL	0.8462 mL	2.1155 mL
	30 mM	0.0705 mL	0.3526 mL	0.7052 mL	1.7629 mL
	40 mM	0.0529 mL	0.2644 mL	0.5289 mL	1.3222 mL
	50 mM	0.0423 mL	0.2116 mL	0.4231 mL	1.0578 mL
	60 mM	0.0353 mL	0.1763 mL	0.3526 mL	0.8815 mL
	80 mM	0.0264 mL	0.1322 mL	0.2644 mL	0.6611 mL
	100 mM	0.0212 mL	0.1058 mL	0.2116 mL	0.5289 mL

Keywords

Echinocystic acid

3 Cited Publications

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Echinocystic acid

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Publications Citing Use of MedChemExpress (MCE) Echinocystic acid

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Echinocystic acid Related Classifications

HY-N0271 Related Classifications