Cat. No.: HY-117078

Justicidin A

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justicidin A is a nature product that could be isolated form Justicia procumbens. justicidin A decreases the level of Ku70 leading to translocation of Bax from the cytosol to mitochondria to induce apoptosis. justicidin A can be used in research of cancer.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Pureté: 99.75%
CAS No.: 25001-57-4
Formule: C₂₂H₁₈O₇
Masse moléculaire:394.37

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Justicidin A Chemical Structure

CAS No.: 25001-57-4

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Activité biologique
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvant et solubilité
- In Vitro
Pureté et documentation
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Ca-Ski	ED₅₀	3 ng/mL Compound: 5	Cytotoxicity against human CaSki cells after 6 days by MTT assay Cytotoxicity against human CaSki cells after 6 days by MTT assay	[PMID: 10425143]
Ca-Ski	ED₅₀	3 x 10^-3 μg/mL Compound: 5	Cytotoxicity against human CaSki cells after 6 days by MTT assay Cytotoxicity against human CaSki cells after 6 days by MTT assay	[PMID: 10425143]
Ca-Ski	ED₅₀	3 ng/mL Compound: 1	Cytotoxicity against human CaSKi cells by MTT assay Cytotoxicity against human CaSKi cells by MTT assay	[PMID: 11908984]
Ca-Ski	ED₅₀	3 x 10^-3 μg/mL Compound: 1	Cytotoxicity against human CaSKi cells by MTT assay Cytotoxicity against human CaSKi cells by MTT assay	[PMID: 11908984]
Hep 3B2	ED₅₀	0.29 ng/mL Compound: 1	Cytotoxicity against human Hep3B cells by MTT assay Cytotoxicity against human Hep3B cells by MTT assay	[PMID: 11908984]
Hep 3B2	ED₅₀	2.9 x 10^-2 μg/mL Compound: 1	Cytotoxicity against human Hep3B cells by MTT assay Cytotoxicity against human Hep3B cells by MTT assay	[PMID: 11908984]
HepG2	ED₅₀	0.2 ng/mL Compound: 1	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 11908984]
HepG2	ED₅₀	2 x 10^-2 μg/mL Compound: 1	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 11908984]
HepG2	IC₅₀	163 μM Compound: 2	Cytotoxicity against Homo sapiens (human) HepG2 cells after 3 days by MTT assay Cytotoxicity against Homo sapiens (human) HepG2 cells after 3 days by MTT assay	10.1007/s00044-009-9172-1
HT-29	IC₅₀	>10 μM Compound: 6	Cytotoxicity against human HT-29 cells Cytotoxicity against human HT-29 cells	[PMID: 24937209]
HT-3	ED₅₀	1.8 ng/mL Compound: 5	Cytotoxicity against human HT-3 cells after 6 days by MTT assay Cytotoxicity against human HT-3 cells after 6 days by MTT assay	[PMID: 10425143]
HT-3	ED₅₀	1.8 x 10^-3 μg/mL Compound: 5	Cytotoxicity against human HT-3 cells after 6 days by MTT assay Cytotoxicity against human HT-3 cells after 6 days by MTT assay	[PMID: 10425143]
HT-3	IC₅₀	0.0018 μg/mL Compound: 110	Antiproliferative activity against human HT3 cells assessed as reduction in cell viability after 6 days by MTT assay Antiproliferative activity against human HT3 cells assessed as reduction in cell viability after 6 days by MTT assay	[PMID: 30830783]
KB	ED₅₀	<1 μg/mL Compound: 9	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 2849640]
KB	ED₅₀	<1 μg/mL Compound: 1	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 3734816]
Platelet	IC₅₀	31.7 μM Compound: 3	Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet-rich blood by turbidimetric method Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet-rich blood by turbidimetric method	[PMID: 8988600]
PLC-PRF-5	ED₅₀	2.2 ng/mL Compound: 5	Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay	[PMID: 10425143]
PLC-PRF-5	ED₅₀	2.2 x 10^-3 μg/mL Compound: 5	Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay	[PMID: 10425143]
SiHa	ED₅₀	7.4 ng/mL Compound: 5	Cytotoxicity against human SiHa cells after 6 days by MTT assay Cytotoxicity against human SiHa cells after 6 days by MTT assay	[PMID: 10425143]
SiHa	ED₅₀	7.4 x 10^-3 μg/mL Compound: 5	Cytotoxicity against human SiHa cells after 6 days by MTT assay Cytotoxicity against human SiHa cells after 6 days by MTT assay	[PMID: 10425143]
SiHa	ED₅₀	7.4 ng/mL Compound: 1	Cytotoxicity against human SiHa cells by MTT assay Cytotoxicity against human SiHa cells by MTT assay	[PMID: 11908984]
SiHa	ED₅₀	7.4 x 10^-3 μg/mL Compound: 1	Cytotoxicity against human SiHa cells by MTT assay Cytotoxicity against human SiHa cells by MTT assay	[PMID: 11908984]
T-24	ED₅₀	2 ng/mL Compound: 5	Cytotoxicity against human T24 cells after 6 days by MTT assay Cytotoxicity against human T24 cells after 6 days by MTT assay	[PMID: 10425143]
T-24	ED₅₀	2 x 10^-3 μg/mL Compound: 5	Cytotoxicity against human T24 cells after 6 days by MTT assay Cytotoxicity against human T24 cells after 6 days by MTT assay	[PMID: 10425143]

In Vitro

justicidin A (0-2.5 μM; 6 days) inhibits cell growth in colorectal cancer cells with IC₅₀ values of 0.110, 0.400, 0.020, 1.540 and 0.004 μM for HT-29, HCT 116, SiHa, MCF7 and T24 cells, respectively^[1].
justicidin A (0.75 and 5 μM; 0-72 h) induces apoptosis in colorectal cancer cells^[1].
justicidin A (0.75 and 5 μM; 0-72 h) induces the cleavage of caspases and their target proteins in human colorectal cancer cells^[1].
justicidin A (0.75 and 5 μM; 0-72 h) damages mitochondria and releases cyto c and Smac from mitochondria^[1].
justicidin A (0.75 and 5 μM; 0-96 h) decreases the level of Ku70 leading to translocation of Bax from the cytosol to mitochondria^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HT-29, HCT 116, SiHa, MCF7 and T24 cells
Concentration:

0-2.5 μM
Incubation Time:

6 days
Result:

Inhibited cell growth in a dose-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

HT-29 and HCT 116 cells
Concentration:

0.75 μM (HT-29 cells) and 5 μM (HCT 116 cells)
Incubation Time:

0, 12, 16, 20, 24, 48, and 72 hours
Result:

Induced apoptosis in a time-dependent manner.
Increased the population of sub-G 1 cells in a time-dependent manner.

Western Blot Analysis^[1]

Cell Line:

HT-29 and HCT 116 cells
Concentration:

0.75 μM (HT-29 cells) and 5 μM (HCT 116 cells)
Incubation Time:

0, 12, 16, 20, 24, 48, 72, and 96 hours
Result:

Decreased the amount of cytosolic Ku70 in HCT 116 cells and decreased (0.6-fold) at 48 h and reached the lowest level (0.33-fold) at 96 h.

Western Blot Analysis^[1]

Cell Line:

HT-29 and HCT 116 cells
Concentration:

0.75 μM (HT-29 cells) and 5 μM (HCT 116 cells)
Incubation Time:

0, 12, 16, 20, 24, 48, and 72 hours
Result:

Induced the cleavage of caspases and their target proteins.

In Vivo

justicidin A (6.2 mg/kg; p.o.; daily, for 56 days) suppresses the growth of human colorectal cancer cells in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

NOD-SCID mice with HT-29 cells xenograftes^[1]
Dosage:

6.2 mg/kg
Administration:

Oral administration; daily, for 56 days
Result:

Inhibited tumor growth and decreased tumor weight.

Chemical Information

CAS No. 25001-57-4
Appearance Solid
Masse moléculaire 394.37
Formule C₂₂H₁₈O₇
Color White to off-white
SMILES

O=C1OCC2=C(OC)C3=CC(OC)=C(OC)C=C3C(C4=CC=C(OCO5)C5=C4)=C12
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité

In Vitro:

DMSO : 16.67 mg/mL (42.27 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5357 mL

12.6784 mL

25.3569 mL

5 mM

0.5071 mL

2.5357 mL

5.0714 mL

10 mM

0.2536 mL

1.2678 mL

2.5357 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (255 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5357 mL	12.6784 mL	25.3569 mL	63.3922 mL
	5 mM	0.5071 mL	2.5357 mL	5.0714 mL	12.6784 mL
	10 mM	0.2536 mL	1.2678 mL	2.5357 mL	6.3392 mL
	15 mM	0.1690 mL	0.8452 mL	1.6905 mL	4.2261 mL
	20 mM	0.1268 mL	0.6339 mL	1.2678 mL	3.1696 mL
	25 mM	0.1014 mL	0.5071 mL	1.0143 mL	2.5357 mL
	30 mM	0.0845 mL	0.4226 mL	0.8452 mL	2.1131 mL
	40 mM	0.0634 mL	0.3170 mL	0.6339 mL	1.5848 mL

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Justicidin A

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mongolian Medicine Compound Library

High-Efficiency Gene Editing Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Activité biologique

Chemical Information

Solvant et solubilité

Pureté et documentation

Help & FAQs

Complete Stock Solution Preparation Table

Justicidin A

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mongolian Medicine Compound Library

High-Efficiency Gene Editing Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Activité biologique

Chemical Information

Solvant et solubilité

Pureté et documentation

Help & FAQs

Complete Stock Solution Preparation Table

Justicidin A Related Classifications

HY-117078 Related Classifications