PF-739
Based on 1 publication(s) in Google Scholar
PF-739 is an orally active and non-selective activator of AMPK. PF-739 activates 12 heterotrimeric AMPK complexes and significantly reduces the level of glucose in plasma complexes.
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- Pureté: 99.79%
- CAS No.: 1852452-14-2
- Formule: C23H23ClN2O5
- Masse moléculaire:442.89
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) PF-739
MoreVoir tous les produits spécifiques à Isoform AMPK
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Activité biologique
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AMPK α1β1γ1 8.99 nM (EC50) |
AMPK α1β2γ1 126 nM (EC50) |
AMPK α2β1γ1 5.23 nM (EC50) |
AMPK α2β2γ1 42.2 nM (EC50) |
PF-739 activates α2β1γ1, α2β2γ1, α1β1γ1 and α1β2γ1 with EC50 values of 5.23 nM, 42.2 nM, 8.99 nM and 126 nM, respectively[1].
PF-739 (0-1000 nM) increases the phosphorylation of AMPK substrate in primary rat hepatocytes and myotubes of primary human cardiac cell line with dose-dependent manner[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice[2].
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Dosage:30, 100, 300 or 1000 mg/kg.
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Administration:Oral gavage or subcutaneous injection; single does.
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Result:Increased AMPK activity in skeletal muscle, AMPK phosphorylation in liver tissue and the expression of transcription factors Ppargc1a, Nr4a1, Nr4a3 in glucose metabolism pathway, and decreased plasma insulin and blood glucose.
Chemical Information
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CAS No. 1852452-14-2
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Appearance Solid
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Masse moléculaire 442.89
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Formule C23H23ClN2O5
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Color White to light yellow
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SMILES
O[C@H]1[C@]([H])([C@@]2(OC1)[H])OC[C@H]2OC3=NC4=CC(C5=CC=C(C=C5)C6(CC6)CO)=C(C=C4N3)Cl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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JCI Insight
2026 May 8;11(9):e200168. PMID: 42100877
Solvant et solubilité
DMSO : 100 mg/mL (225.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.64 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.64 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2579 mL | 11.2895 mL | 22.5790 mL | 56.4474 mL |
| 5 mM | 0.4516 mL | 2.2579 mL | 4.5158 mL | 11.2895 mL | |
| 10 mM | 0.2258 mL | 1.1289 mL | 2.2579 mL | 5.6447 mL | |
| 15 mM | 0.1505 mL | 0.7526 mL | 1.5053 mL | 3.7632 mL | |
| 20 mM | 0.1129 mL | 0.5645 mL | 1.1289 mL | 2.8224 mL | |
| 25 mM | 0.0903 mL | 0.4516 mL | 0.9032 mL | 2.2579 mL | |
| 30 mM | 0.0753 mL | 0.3763 mL | 0.7526 mL | 1.8816 mL | |
| 40 mM | 0.0564 mL | 0.2822 mL | 0.5645 mL | 1.4112 mL | |
| 50 mM | 0.0452 mL | 0.2258 mL | 0.4516 mL | 1.1289 mL | |
| 60 mM | 0.0376 mL | 0.1882 mL | 0.3763 mL | 0.9408 mL | |
| 80 mM | 0.0282 mL | 0.1411 mL | 0.2822 mL | 0.7056 mL | |
| 100 mM | 0.0226 mL | 0.1129 mL | 0.2258 mL | 0.5645 mL |
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