Cat. No.: HY-N0279

Cardamonin

(Synonyms: Cardamomin; Alpinetin chalcone)

18 Cited Publications

Customer Review

Based on 18 publication(s) in Google Scholar

Cardamonin can be found from cardamom, and can target various signaling molecules, transcriptional factors, cytokines and enzymes. Cardamonin can inhibit mTOR, NF-κB, Akt, STAT3, Wnt/β-catenin and COX-2. Cardamonin shows anticancer, anti-inflammatory, antimicrobial and antidiabetic activities.

For research use only. We do not sell to patients.

Purity: 99.80%
CAS No.: 18956-16-6
Formula: C₁₆H₁₄O₄
Molecular Weight:270.28

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Cardamonin Chemical Structure

CAS No.: 18956-16-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (18)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All NF-κB Isoforms :

All STAT Isoforms :

All Wnt Isoforms :

All Bcl-2 Family Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
B16-F10	IC₅₀	0.6 μM Compound: 139	Antiinflammatory activity in mouse B16-F10 cells assessed as decrease in LPS-induced TNFalpha expression by Western blot analysis Antiinflammatory activity in mouse B16-F10 cells assessed as decrease in LPS-induced TNFalpha expression by Western blot analysis	[PMID: 37683361]
CCRF-CEM	IC₅₀	≤10 μM Compound: 64	Cytotoxicity in human CCRF-CEM cells by resazurin reduction assay Cytotoxicity in human CCRF-CEM cells by resazurin reduction assay	[PMID: 28793973]
DLD-1	IC₅₀	41.8 μM Compound: 6	Cytotoxicity against human DLD1 cells co-transfected with Sac1 after 24 hrs Cytotoxicity against human DLD1 cells co-transfected with Sac1 after 24 hrs	[PMID: 19682913]
L929	IC₅₀	2 nM Compound: PAP-1	Inhibition of human voltage-gated potassium channel Kv1.3 expressed in L929 cells by whole-cell patch clamp assay Inhibition of human voltage-gated potassium channel Kv1.3 expressed in L929 cells by whole-cell patch clamp assay	[PMID: 20971642]
RAW264.7	IC₅₀	2.2 μM Compound: Cardamonin	Inhibition of LPS-induced NO production in mouse RAW264.7 cells pretreated for 30 mins followed by LPS challenge measured after 12 hrs by Griess assay Inhibition of LPS-induced NO production in mouse RAW264.7 cells pretreated for 30 mins followed by LPS challenge measured after 12 hrs by Griess assay	[PMID: 28011215]
RAW264.7	IC₅₀	2.2 μM Compound: Cardamonin	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 12 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 12 hrs by Griess assay	[PMID: 28688956]
RAW264.7	IC₅₀	11.48 μM Compound: 8a	Anti-inflammatory activity in Mus musculus (mouse) RAW264.7 cells assessed as inhibition of IFN-gamma/LPS-induced NO production after 17 to 20 hr by Griess assay Anti-inflammatory activity in Mus musculus (mouse) RAW264.7 cells assessed as inhibition of IFN-gamma/LPS-induced NO production after 17 to 20 hr by Griess assay	10.1007/s00044-011-9706-1
THP-1	IC₅₀	1.1 μM Compound: 139	Antiinflammatory activity in human THP-1 cells assessed as decrease in LPS-induced TNFalpha expression by Western blot analysis Antiinflammatory activity in human THP-1 cells assessed as decrease in LPS-induced TNFalpha expression by Western blot analysis	[PMID: 37683361]

In Vitro

Cardamonin (5-40 μM, 24 or 48 h) treatment inhibits gastric cancer cell growth^[3].
Cardamonin (10-30 μM, 24 or 48 h) treatment can regulate the expression of apoptosis relative protein^[3].
Cardamonin (10-30 μM, 24 or 48 h) treatment can inhibit STAT3 phosphorylation^[3].
Cardamonin (0-100 μM, 24 h) treatment improves the anti-oxidative capacity in HL-1 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

AGS, MGC-803, BGC-823 cells
Concentration:

5, 10, 20, 30, 40 μM
Incubation Time:

24 or 48 hours
Result:

Inhibited cell growth in a concentration-dependent manner.

Western Blot Analysis^[3]

Cell Line:

AGS, MGC-803, BGC-823 cells
Concentration:

10, 20, 30 μM
Incubation Time:

24 or 48 hours
Result:

Down-regulated Bcl-2, increased Bax protein expression, and increased the protein expression levels of Caspase-3.

Western Blot Analysis^[3]

Cell Line:

AGS cells
Concentration:

10, 20, 30 μM
Incubation Time:

24 or 48 hours
Result:

Suppressed the phosphorylation level of STAT3.

Western Blot Analysis^[4]

Cell Line:

HL-1 cells
Concentration:

0, 25, 50, 100 μM
Incubation Time:

24 hours
Result:

Showed anti-oxidant effects in doxorubicin-stimulated cardiomyocytes.

In Vivo

Cardamonin (oral gavage; 20, 40 or 80 mg/kg; once) treatments alleviates doxorubicin-induced cardiotoxicity and inhibits apoptosis in doxorubicin-challenged mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/6 J mice (8 weeks old, 20-22 g)^[4]
Dosage:

20, 40 or 80 mg/kg
Administration:

Oral gavage; 20, 40 or 80 mg/kg; once
Result:

Rescued the reduction of LVEF% and LVFS% by doxorubicin, reduced the increasement of serum LDH, CK-MB and Tn-T levels by doxorubicin in a dose-dependent manner, improved doxorubicin-induced histological changes, and attenuated collagen accumulation in cardiac sections by doxorubicin in a dose-dependent manner.

Animal Model:

Male C57BL/6 J mice (8 weeks old, 20-22 g)^[4]
Dosage:

20, 40 or 80 mg/kg
Administration:

Oral gavage; 20, 40 or 80 mg/kg; once
Result:

Rescued Bcl-2, and inhibited Bax and Caspase-3 cleavage in heart tissues from doxorubicin-challenged mice.

Chemical Information

CAS No. 18956-16-6
Appearance Solid
Molecular Weight 270.28
Formula C₁₆H₁₄O₄
Color Light yellow to yellow
SMILES

O=C(C1=C(OC)C=C(O)C=C1O)/C=C/C2=CC=CC=C2
Synonyms

Cardamomin; Alpinetin chalcone
Structure Classification
- Flavonoids
- Chalcones
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (18)

All WB

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Journal Impact Factor

Acta Pharm Sin B

Sulfation of chondroitin and bile acids converges to antagonize Wnt/ β-catenin signaling and inhibit APC deficiency-induced gut tumorigenesis. [Abstract]

2024 Mar;14(3):1241-1256. PMID: 38487006
Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186
Pharmacol Res

Cardamonin retards progression of autosomal dominant polycystic kidney disease via inhibiting renal cyst growth and interstitial fibrosis. [Abstract]

2020 May;155:104751. PMID: 32151678
Phytomedicine

Cardamonin inhibits atrial remodeling in mice by upregulating the expression of USP18. [Abstract]

2025 Dec:149:157512. PMID: 41240540
Phytomedicine

Cardamonin intervenes in myocardial hypertrophy progression by regulating Usp18. [Abstract]

2024 Aug 19:134:155970. PMID: 39178681
Biomed Pharmacother

Cardamonin protects against doxorubicin-induced cardiotoxicity in mice by restraining oxidative stress and inflammation associated with Nrf2 signaling. [Abstract]

2020 Feb;122:109547. PMID: 31918264

Cardamonin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Feb;122:109547. [Abstract]

Cardamonin promotes the Nrf2-ARE regulated cytoprotective genes in HL-1 cells dependent on Nrf2. western blot analysis were used to determine Nrf2, HO1, NQO1 and GCLM expression levels in HL-1 cells treated with the indicated concentrations of CAR (0, 25, 50 and 100 μM) for 24 h.

Cardamonin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Feb;122:109547. [Abstract]

Cardamonin promotes the Nrf2-ARE regulated cytoprotective genes in HL-1 cells dependent on Nrf2. Western blot analysis of Nrf2, HO1, NQO1 and GCLM.
Antioxidants (Basel)

A Combination of Cardamonin and Doxorubicin Selectively Affect Cell Viability of Melanoma Cells: An In Vitro Study. [Abstract]

2024 Jul 19;13(7):864. PMID: 39061932
Phytother Res

Cardamonin attenuates cerebral ischemia/reperfusion injury by activating the HIF-1α/VEGFA pathway. [Abstract]

2022 Apr;36(4):1736-1747. PMID: 35142404
J Agric Food Chem

Nephroprotective Effects of Cardamonin on Renal Ischemia Reperfusion Injury/UUO-Induced Renal Fibrosis. [Abstract]

2023 Sep 13;71(36):13284-13303. PMID: 37646396
Food Funct

Cardamonin protects against diabetic cardiomyopathy by activating macrophage NRF2 signaling through molecular interaction with KEAP1. [Abstract]

2024 Nov 11;15(22):11083-11095. PMID: 39431579
Food Funct

Cardamonin inhibits osteogenic differentiation of human valve interstitial cells and ameliorates aortic valve calcification via interfering in the NF-κB/NLRP3 inflammasome pathway. [Abstract]

2021 Nov 29;12(23):11808-11818. PMID: 34766179
Nutrients

Inhibitory Effect of Tangeretin and Cardamonin on Human Intestinal SGLT1 Activity In Vitro and Blood Glucose Levels in Mice In Vivo. [Abstract]

2021 Sep 26;13(10):3382. PMID: 34684383
Int J Mol Sci

Integrating Network Pharmacology, Machine Learning, and Experimental Validation to Elucidate the Mechanism of Cardamonin in Treating Idiopathic Pulmonary Fibrosis. [Abstract]

2025 Dec 25;27(1):249. PMID: 41516127
ACS Omega

Receptor-Selective Modulation of Cannabinoid Signaling by Cardamonin: Integrating Molecular Dynamics, Free Energy Calculations, and Behavioral Validation. [Abstract]

2026 Apr 13;11(16):23820-23832. PMID: 42077871
Sci Rep

Cardamonin inhibits the growth and stemness of osteosarcoma stem cells by inducing autophagy and inhibiting the Wnt/β-catenin signaling pathway. [Abstract]

2025 Aug 12;15(1):29484. PMID: 40796594
Toxicol Appl Pharmacol

Cardamonin attenuates osteoporosis progression and promotes osteogenic differentiation of bone mesenchymal stem cells by upregulating TCF4 expression. [Abstract]

2026 Mar:508:117731. PMID: 41581571
Toxicol In Vitro

The natural chalcone cardamonin selectively induces apoptosis in human neuroblastoma cells. [Abstract]

2023 Sep:91:105625. PMID: 37268255
Neurol Res

Neuroregenerative effects of cardamonin in a rat model of sciatic nerve crush injury. [Abstract]

2025 Nov 25:1-17. PMID: 41289366

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (462.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6999 mL

18.4993 mL

36.9987 mL

5 mM

0.7400 mL

3.6999 mL

7.3997 mL

10 mM

0.3700 mL

1.8499 mL

3.6999 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.17 mg/mL (8.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Javaria Nawaz, et al. Cardamonin: A new player to fight cancer via multiple cancer signaling pathways. Life Sci. 2020 Jun 1;250:117591. [Content Brief]

[2]. Kai Wang, et al. Cardamonin, a natural flavone, alleviates inflammatory bowel disease by the inhibition of NLRP3 inflammasome activation via an AhR/Nrf2/NQO1 pathway. Biochem Pharmacol. 2018 Sep;155:494-509. [Content Brief]

[3]. Zheng Wang, et al. Cardamonin exerts anti-gastric cancer activity via inhibiting LncRNA-PVT1-STAT3 axis. Biosci Rep. 2019 May 17;39(5):BSR20190357. [Content Brief]

[4]. Wang Qi, et al. Cardamonin protects against doxorubicin-induced cardiotoxicity in mice by restraining oxidative stress and inflammation associated with Nrf2 signaling. Biomed Pharmacother. 2020 Feb;122:109547. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6999 mL	18.4993 mL	36.9987 mL	92.4967 mL
	5 mM	0.7400 mL	3.6999 mL	7.3997 mL	18.4993 mL
	10 mM	0.3700 mL	1.8499 mL	3.6999 mL	9.2497 mL
	15 mM	0.2467 mL	1.2333 mL	2.4666 mL	6.1664 mL
	20 mM	0.1850 mL	0.9250 mL	1.8499 mL	4.6248 mL
	25 mM	0.1480 mL	0.7400 mL	1.4799 mL	3.6999 mL
	30 mM	0.1233 mL	0.6166 mL	1.2333 mL	3.0832 mL
	40 mM	0.0925 mL	0.4625 mL	0.9250 mL	2.3124 mL
	50 mM	0.0740 mL	0.3700 mL	0.7400 mL	1.8499 mL
	60 mM	0.0617 mL	0.3083 mL	0.6166 mL	1.5416 mL
	80 mM	0.0462 mL	0.2312 mL	0.4625 mL	1.1562 mL
	100 mM	0.0370 mL	0.1850 mL	0.3700 mL	0.9250 mL

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Cardamonin

Apoptosis Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

NF-κB Signaling Compound Library

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Publications Citing Use of MedChemExpress (MCE) Cardamonin

All NF-κB Isoforms

All STAT Isoforms

All Wnt Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Chemical Information

Publications (18)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs