1. Cell Cycle/DNA Damage
    Stem Cell/Wnt
    Metabolic Enzyme/Protease
    MAPK/ERK Pathway
  2. Casein Kinase
    CDK
    SGK
    Ribosomal S6 Kinase (RSK)
  3. CKI-7

CKI-7 

Cat. No.: HY-133028
Handling Instructions

CKI-7 is a potent and ATP-competitive casein kinase 1 (CK1) inhibitor with an IC50 of 6 μM and a Ki of 8.5 μM. CKI-7 is a selective Cdc7 kinase inhibitor. CKI-7 also inhibits SGK, ribosomal S6 kinase-1 (S6K1) and mitogen- and stress-activated protein kinase-1 (MSK1). CKI-7 has a much weaker effect on casein kinase II and other protein kinases.

For research use only. We do not sell to patients.

CKI-7 Chemical Structure

CKI-7 Chemical Structure

CAS No. : 120615-25-0

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10 mM * 1 mL in DMSO USD 157 In-stock
Estimated Time of Arrival: December 31
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25 mg USD 375 In-stock
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100 mg USD 1120 In-stock
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Description

CKI-7 is a potent and ATP-competitive casein kinase 1 (CK1) inhibitor with an IC50 of 6 μM and a Ki of 8.5 μM. CKI-7 is a selective Cdc7 kinase inhibitor. CKI-7 also inhibits SGK, ribosomal S6 kinase-1 (S6K1) and mitogen- and stress-activated protein kinase-1 (MSK1). CKI-7 has a much weaker effect on casein kinase II and other protein kinases[1][2][3][4].

IC50 & Target[1][2][3]

CK1

6 μM (IC50)

CK1

8.5 μM (Ki)

Cdc7

 

SGK

 

S6K1

 

MSK1

 

In Vitro

CKI-7 (0.1-10 μM; 5 days; ES cells) treatment significantly increases the expression of the early neuroectodermal marker Sox1 and the number of cells positive for the neural markers nestin and βIII-tubulin, in a concentration-dependent manner[1].
CKI-7 (5 μM; 5 days; ES cells) treatment suppresses SFEB-induced β-catenin stabilization on day 5, indicating that CKI-7 inhibits Wnt signaling[1].

RT-PCR[1]

Cell Line: Mouse ES cells
Concentration: 0.1-10 μM
Incubation Time: 5 days
Result: Significantly increased the expression of the early neuroectodermal marker Sox1 and the number of cells positive for the neural markers nestin and βIII-tubulin, in a concentration-dependent manner.

Western Blot Analysis[1]

Cell Line: Mouse ES cells
Concentration: 5 μM
Incubation Time: 5 days
Result: Suppressed SFEB-induced β-catenin stabilization on day 5.
In Vivo

In vivo dose-dependent anti-tumor activity of CKI-7 is demonstrated in a SCID-Beige mouse systemic tumor model utilzing a recently isolated Philadelphia chromosome positive acute lymphoblastic leukemia cell line. Standard cell cycle synchronization studies established that exposure to CKI-7 results in cell cycle dependent caspase 3 activation and apoptotic cell death[2].

Molecular Weight

285.75

Formula

C₁₁H₁₂ClN₃O₂S

CAS No.

120615-25-0

SMILES

O=S(C1=CC=C(Cl)C2=C1C=NC=C2)(NCCN)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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Keywords:

CKI-7CKI7CKI 7Casein KinaseCDKSGKRibosomal S6 Kinase (RSK)Cyclin dependent kinaseSerum-glucocorticoid regulated kinaseSerum and glucocorticoid-regulated kinaseS6KWntSox1βIII-tubulinnestinneuraldifferentiationCK1S6K1MSK1Inhibitorinhibitorinhibit

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CKI-7
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