2. Cytoskeleton Cell Cycle/DNA Damage Neuronal Signaling Membrane Transporter/Ion Channel
  3. Arp2/3 Complex DNA/RNA Synthesis Calcium Channel
  4. Dihydrocytochalasin B

Dihydrocytochalasin B is an Actin disruptor. Dihydrocytochalasin B disrupts actin microfilament bundles, inhibits actin polymerization, and alters intracellular actin cytoskeletal structures. Dihydrocytochalasin B blocks the initiation of DNA synthesis. Dihydrocytochalasin B inhibits Calcium transport. Dihydrocytochalasin B inhibits cytokinesis and alters cell morphology. Dihydrocytochalasin B can be used in studies related to rickets.

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Dihydrocytochalasin B

Dihydrocytochalasin B Chemical Structure

CAS No. : 39156-67-7

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500 μg In-stock

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Dihydrocytochalasin B Related Antibodies

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Description

Dihydrocytochalasin B is an Actin disruptor. Dihydrocytochalasin B disrupts actin microfilament bundles, inhibits actin polymerization, and alters intracellular actin cytoskeletal structures. Dihydrocytochalasin B blocks the initiation of DNA synthesis. Dihydrocytochalasin B inhibits Calcium transport. Dihydrocytochalasin B inhibits cytokinesis and alters cell morphology. Dihydrocytochalasin B can be used in studies related to rickets[1][2][3].

In Vitro

Dihydrocytochalasin B (2-150×10-7 M; 24 h) reversibly inhibits serum-induced initiation of DNA synthesis in serum-starved Swiss/3T3 mouse fibroblasts[1].
Dihydrocytochalasin B (1-7×10-7 M) does not inhibit 3H-2-deoxyglucose uptake by proliferating Swiss/3T3 mouse fibroblasts[1].
Dihydrocytochalasin B (2-10×10-7 M; 24 h) inhibits serum-induced initiation of DNA synthesis in serum-starved Swiss/3T3 mouse fibroblasts, and this effect is fully reversible[1].
Dihydrocytochalasin B (5-25 μg/mL; 30-60 min) inhibits active calcium transport in everted ileal sacs of young rabbits in a dose-dependent manner; after 60 min of incubation, a significant increase in 45Ca accumulation in mucosal scrapings is observed at the dose of 25 μg/mL[2].
Dihydrocytochalasin B (4-10 μM; 1 h) induces dose-dependent morphological changes identical to those induced by cytochalasin B (including cytoplasmic blebbing, nuclear extrusion, dendritization, and cell rounding) in BALB/c 3T3 cells, but it has slightly lower potency and exhibits an additive effect with cytochalasin B[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dihydrocytochalasin B Related Antibodies

Immunofluorescence[1]

Cell Line: serum-arrested Swiss/3T3 mouse fibroblasts
Concentration: 1×10-7 M, 2×10-7 M, 5×10-7 M, 7.8×10-7 M, 10×10-7 M
Incubation Time: 24 h
Result: Caused concentration-dependent disruption of actin microfilament bundles, loss of cell-substratum attachment sites, and progressive cell rounding.
Detected no changes in actin structure or cell shape at 1×10-7 M.
Made microfilament bundles fewer, thinner, and shorter, with cells assuming a rectangular shape at 2×10-7 M.
Left only peripheral microfilament bundles remaining, with cells adopting a spindle shape at 5×10-7 M.
Resulted in few to no actin microfilament bundles visible, with cells becoming rounded with blebby surfaces at 7.8 and 10×10-7 M.
Increased the percentage of cells with disorganized actin structure with concentration: 11% at 1×10-7 M, 30% at 2×10-7 M, 43% at 5×10-7 M, 60% at 7.8×10-7 M, and 89% at 10×10-7 M.
Showed correlation between actin disorganization and inhibition of DNA synthesis.
Molecular Weight

481.62

Formula

C29H39NO5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1[C@]2(O3)[C@]([C@@H](C([C@@H](O)[C@]2([H])/C=C/C[C@@H](CCC[C@@H](O)CCC3=O)C)=C)C)([H])[C@H](CC4=CC=CC=C4)N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMF : 30 mg/mL (62.29 mM; Need ultrasonic and warming)

DMSO : ≥ 20 mg/mL (41.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0763 mL 10.3816 mL 20.7633 mL
5 mM 0.4153 mL 2.0763 mL 4.1527 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.0%

SDS (644 KB)

COA (253 KB) RP-HPLC (1076 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / DMF 1 mM 2.0763 mL 10.3816 mL 20.7633 mL 51.9081 mL
5 mM 0.4153 mL 2.0763 mL 4.1527 mL 10.3816 mL
10 mM 0.2076 mL 1.0382 mL 2.0763 mL 5.1908 mL
15 mM 0.1384 mL 0.6921 mL 1.3842 mL 3.4605 mL
20 mM 0.1038 mL 0.5191 mL 1.0382 mL 2.5954 mL
25 mM 0.0831 mL 0.4153 mL 0.8305 mL 2.0763 mL
30 mM 0.0692 mL 0.3461 mL 0.6921 mL 1.7303 mL
40 mM 0.0519 mL 0.2595 mL 0.5191 mL 1.2977 mL
DMF 50 mM 0.0415 mL 0.2076 mL 0.4153 mL 1.0382 mL
60 mM 0.0346 mL 0.1730 mL 0.3461 mL 0.8651 mL
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Dihydrocytochalasin B Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dihydrocytochalasin B39156-67-7Arp2/3 ComplexDNA/RNA SynthesisCalcium ChannelActin-related protein 2/3 complex Ca2+ channelsCa channelsSV40-transformed Swiss/3T3 mouse fibroblastsM-MuSV-transformed Swiss/3T3 mouse fibroblastsintestinal absorptive cellsDNA synthesisactin microfilament bundlesactinricketsBALB/c 3T3 cellsSwiss/3T3 mouse fibroblastsactin polymerizationInhibitorinhibitorinhibit

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