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Cat. No.: HY-175037

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DNH28

DNH28 is a potent NF-κB and MAPK inhibitor with an IC₅₀ of 0.93 μM against HepG2 cells. DNH28 promotes apoptosis by down-regulating the expression of Bcl-2, up-regulating the expression of BAX and Cleaved-caspase-3. DNH28 blocks the cell cycle and inhibits migration. DNH28 can be used for the study of hepatocellular carcinoma (HCC).

For research use only. We do not sell to patients.

Formula: C₂₃H₂₄ClF₃N₂O
Molecular Weight:436.90

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

DNH28 Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

Caspase 3

Bax

Bcl-2

NF-κB

All NF-κB Isoforms :

All p38 MAPK Isoforms :

All Bcl-2 Family Isoforms :

All Caspase Isoforms :

In Vitro

DNH28 (Compound 28) exhibits lower toxicity toward THLE-3 cells (IC₅₀ = 18.16 μM)^[1].
DNH28 (0.46-1.84 μM, 24 h) inhibits HepG2 cells proliferation by inducing apoptosis and dose-dependently inhibits HepG2 cells in the G2/M phase^[1].
DNH28 (0.46-1.84 μM, 24 h) inhibits HepG2 cell migration dose-dependently, and it completely inhibits the migration ability at a concentration of 1.84 μM^[1].
DNH28 (1.84 μM, 24 h) effectively inhibits the nuclear translocation of p65 induced by TNF-α^[1].
DNH28 (0.46-1.84 μM, 24 h) exerts anti-HCC effects by inhibiting the activation of NF-κB and MAPK signaling pathways^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

0.46, 0.92 and 1.84 μM
Incubation Time:

24 h
Result:

Induced apoptosis dose-dependently.
Reached 45.90% of apoptotic cells at 1.84 μM (compared with 14.57% in the control group).

Cell Cycle Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

0.46, 0.92 and 1.84 μM
Incubation Time:

24 h
Result:

Reached 76.22% G2/M phase ratio at 1.84 μM.

Cell Migration Assay ^[1]

Cell Line:

HepG2 cells
Concentration:

0.46, 0.92 and 1.84 μM
Incubation Time:

24 h
Result:

Partially suppressed cell migration, whereas at 1.84 μM, the scratch width remained unchanged.

RT-PCR^[1]

Cell Line:

HepG2 cells
Concentration:

0.46, 0.92 and 1.84 μM
Incubation Time:

24 h
Result:

Increased the expression of pro-apoptotic mRNA levels BAX and C-caspase-3.
Reduced the expression of the anti-apoptotic mRNA levels of BCL-2.

Western Blot Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

0.46, 0.92 and 1.84 μM
Incubation Time:

24 h
Result:

Exhibited dose-dependent inhibition of the phosphorylation of p65, IκB-α, p38 and ERK.
Significantly down-regulated the activation of the NF-κB and MAPK signaling pathways

Chemical Information

Molecular Weight 436.90
Formula C₂₃H₂₄ClF₃N₂O
SMILES

CCN1CCN(C2=CC(CC/C(C3=O)=C\C4=CC=C(F)C(F)=C4F)=C3C=C2)CC1.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yu L, et al. Novel 3,4-dihydronaphthalen-1(2H)-one derivatives promote apoptosis and inhibit migration of hepatocellularcarcinoma cells via inhibition of NF-κB and MAPK signaling pathways. Eur J Med Chem. 2025 Oct 15;296:117898. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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DNH28

All NF-κB Isoforms

All p38 MAPK Isoforms

All Bcl-2 Family Isoforms

All Caspase Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

DNH28 Related Classifications

HY-175037 Related Classifications