Dopamine-PEG3400-N3
Dopamine-PEG3400-N3 (Dopamine-PEG3400-Azide) is a compound composed of dopamine, PEG segments and an azide group (-N3). Dopamine-PEG3400-N3 can be used as a crosslinker to connect different materials together to build new composite materials and improve the performance of materials. Dopamine-PEG3400-N3 is a click chemistry reagent. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing an alkyne group.
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- Peso molecular:3400 (Average)
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Chemical Information
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Actividad biológica
Descripciòn
Chemical Information
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Peso molecular 3400 (Average)
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SMILES
O=C(NCCC1=CC(O)=C(C=C1)O)COCCN=[N+]=[N-].[n]
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Synonyms
Dopamine-PEG3400-Azide
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureza y Documentación
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Dopamine-PEG3400-N3 Related Classifications
HY-W1123941D Related Classifications
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