Taq DNA polymerase 2.0
Based on 1 Customer Validation
Taq DNA polymerase 2.0 is a thermostable DNA polymerase that can be used in PCR.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
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DNA Polymerase |
PCR system
2.5 μL 10x PCR Buffer; Up to 5 μL of whole blood template DNA; 2 μL 2.5 mM dNTP; 1 μL upstream primer (10 μM); 1 μL downstream primer (10 μM); 0.5 μL Taq DNA polymerase 2.0 The total volume of Nuclease-free water replenishment is 25 μL;
Note: The following reaction system takes 10×PCR Buffer as an example. If 10× high GC buffer is used, it is recommended to increase the dNTA concentration to 4 μL (in a 25 μL system).
Buffer component information:
10×PCR Buffer: 150 mM Tris, 50 0mM KCl,150 mM (NH4)2SO4, 20mM MgSO4, NP40 0.8% (PH 8.8);
10× High GC buffer: 200 mM Tris, 200 mM (NH4)2SO4, 15 mM MgSO4, NP40 0.8%, Betaine (HY-B0710) 1.5 M (PH 8.8).
PCR cycle
1) 95°C for 3 minutes Cycle once;
2) 94°C for 30 seconds; 55°C for 30 seconds; 72°C 45-90 seconds; Cycle 35 to 90 times
3) 72°C for 7 minutes; Cycle once.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Taq DNA polymerase 2.0]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Taq DNA polymerase 2.0 Related Classifications
HY-E70087 Related Classifications
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