Nos3 - nitric oxide synthase 3, endothelial cell Gene

Also Known as eNOS; Nos-3; ecNOS; 2310065A03Rik

Species: Mus musculus

Gene Type: protein coding
Gene ID: 18127

About Nos3

Chromosome 5: 24,569,808-24,589,472 forward strand.GRCm39:CM000998.3

This gene has 2 transcripts (splice variants), 114 orthologues, 5 paralogues and is associated with 243 phenotypes. Broad expression in subcutaneous fat pad adult (RPKM 22.), mammary gland adult (RPKM 2.1) and 26 other tissues.

Summary

Enables nitric-oxide synthase activity and scaffold protein binding activity. Involved in several processes, including heart development; negative regulation of muscle hyperplasia; and smooth muscle hyperplasia. Acts upstream of or within several processes, including lipopolysaccharide-mediated signaling pathway; nitric oxide biosynthetic process; and regulation of metal ion transport. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; cardiovascular system; genitourinary system; hemolymphoid system gland; and nervous system. Used to study aortic valve disease; essential hypertension; and persistent fetal circulation syndrome. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer's disease (multiple); artery disease (multiple); eye disease (multiple); reproductive organ Cancer (multiple); and vasculitis (multiple). Orthologous to human NOS3 (nitric oxide synthase 3). [provided by Alliance of Genome Resources, Apr 2022]

Nos3 Products (1)

mRNA Protein Name
NM_008713.4 NP_032739.3 nitric oxide synthase, endothelial

Gene Ontology

  • Molecular Function
  • Biological Process
  • Cellular Component
Molecular Function GO Annotation Evidence References Source
enables nitric-oxide synthase activity IMP
IMP: Inferred from mutant phenotype
15647265 MGI
enables protein binding IPI
IPI: Inferred from physical interaction
22081021 MGI
enables scaffold protein binding IPI
IPI: Inferred from physical interaction
25017399 MGI
Biological Process GO Annotation Evidence References Source
acts upstream of or within angiogenesis IMP
IMP: Inferred from mutant phenotype
15016731 MGI
involved in aortic valve morphogenesis IGI
IGI: Inferred from genetic interaction
27107132 MGI
involved in aortic valve morphogenesis IMP
IMP: Inferred from mutant phenotype
10821808 MGI
involved in blood vessel diameter maintenance IMP
IMP: Inferred from mutant phenotype
9466966 MGI
involved in blood vessel remodeling IMP
IMP: Inferred from mutant phenotype
9466966 MGI
acts upstream of calcium ion transport IMP
IMP: Inferred from mutant phenotype
12074580 MGI
involved in endocardial cushion morphogenesis IGI
IGI: Inferred from genetic interaction
27107132 MGI
acts upstream of establishment of localization in cell IMP
IMP: Inferred from mutant phenotype
12074580 MGI
involved in homeostasis of number of cells within a tissue IGI
IGI: Inferred from genetic interaction
27107132 MGI
acts upstream of or within in utero embryonic development IMP
IMP: Inferred from mutant phenotype
11732991 MGI
acts upstream of or within lipopolysaccharide-mediated signaling pathway IMP
IMP: Inferred from mutant phenotype
15647265 MGI
acts upstream of or within lung development IMP
IMP: Inferred from mutant phenotype
15016731 MGI
involved in negative regulation of biomineral tissue development IMP
IMP: Inferred from mutant phenotype
23583836 MGI
acts upstream of or within negative regulation of calcium ion transport IMP
IMP: Inferred from mutant phenotype
12074580 MGI
involved in negative regulation of cell population proliferation IMP
IMP: Inferred from mutant phenotype
9466966 MGI
acts upstream of or within negative regulation of hydrolase activity IMP
IMP: Inferred from mutant phenotype
12074580 MGI
involved in negative regulation of muscle hyperplasia IMP
IMP: Inferred from mutant phenotype
9466966 MGI
acts upstream of or within negative regulation of potassium ion transport IMP
IMP: Inferred from mutant phenotype
12074580 MGI
acts upstream of or within negative regulation of smooth muscle cell proliferation IMP
IMP: Inferred from mutant phenotype
20554900 MGI
acts upstream of or within nitric oxide biosynthetic process IMP
IMP: Inferred from mutant phenotype
15647265 MGI
acts upstream of or within ovulation from ovarian follicle IMP
IMP: Inferred from mutant phenotype
11779605 MGI
involved in positive regulation of angiogenesis IMP
IMP: Inferred from mutant phenotype
9616228 MGI
acts upstream of positive effect positive regulation of gene expression IGI
IGI: Inferred from genetic interaction
23583836 MGI
acts upstream of positive effect positive regulation of gene expression IMP
IMP: Inferred from mutant phenotype
23583836 MGI
acts upstream of potassium ion transport IMP
IMP: Inferred from mutant phenotype
12074580 MGI
involved in pulmonary valve morphogenesis IGI
IGI: Inferred from genetic interaction
27107132 MGI
involved in regulation of nervous system process IGI
IGI: Inferred from genetic interaction
29353861 MGI
acts upstream of or within regulation of sodium ion transport IMP
IMP: Inferred from mutant phenotype
12074580 MGI
acts upstream of or within regulation of the force of heart contraction by chemical signal IGI
IGI: Inferred from genetic interaction
18951881 MGI
involved in smooth muscle hyperplasia IMP
IMP: Inferred from mutant phenotype
9466966 MGI
involved in ventricular septum morphogenesis IGI
IGI: Inferred from genetic interaction
27107132 MGI
Cellular Component GO Annotation Evidence References Source
located in cytoplasm IDA
IDA: Inferred from direct assay
11732991 MGI
located in nucleus IDA
IDA: Inferred from direct assay
11732991 MGI
EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

Nos3 Protein Structure

NOS_oxygenase_euk

NOS_oxygenase_euk: cd00795 (66 - 478)

Flavodoxin_1

Flavodoxin_1: pfam00258 (520 - 696)

FNR_like

FNR_like: cl06868 (760 - 1162)

  • 0
  • 200
  • 400
  • 600
  • 800
  • 1000
  • 1202 a.a.
Protein Preferred Names Protein Names

nitric oxide synthase, endothelial

  • EC-NOS

  • NOS type III

  • NOSIII

  • cNOS

  • constitutive NOS

  • endothelial NOS

  • endothelial nitric oxide synthase

Related Agents

  • Clinical Phase
  • Pre-clinical Phase

Clinical Phase

Bioactive Molecules (1)
Cat. No. Product Name Effect
Purity Phase
HY-18732 L-NMMA Inhibitor 99.43% Phase 4

Pre-clinical Phase

Bioactive Molecules (5)
Cat. No. Product Name
Effect
Purity
Rare Diseases
HY-N8761 Kihadanin A Inhibitor 97.0% Unkown
HY-RS09441 Nos3 Mouse Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-RS09023 NANOS3 Human Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-RS09440 NOS3 Human Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-RS09442 Nos3 Rat Pre-designed siRNA Set A Pre-designed Set / Unkown

Orthologs Information

Species Symbol Source ID
Homo sapiens Nos3 NCBI NCBI:4846