Glucose 6-phosphate dehydrogenase (yeast, recombinant)
Based on 1 Customer Validation
Glucose 6-phosphate dehydrogenase (yeast, recombinant) (G6PD (yeast, recombinant)) is a recombinant enzyme requiring NADP+ as a coenzyme. Glucose 6-phosphate dehydrogenase (yeast, recombinant) catalyzes the oxidative dehydrogenation of glucose-6-phosphate to form 6-phosphoglucono-δ-lactone, as well as the reduction of NADP+ to NADPH. Glucose 6-phosphate dehydrogenase (yeast, recombinant) relies on the amino acid Arg-52 to bind NADP+. Glucose 6-phosphate dehydrogenase (yeast, recombinant) undergoes competitive inhibition by NADPH and strong inhibition by zinc ions (Zn2+). Glucose 6-phosphate dehydrogenase (yeast, recombinant) is an enzyme in the pentose phosphate pathway, which is one of the glucose metabolic pathways. Glucose 6-phosphate dehydrogenase (yeast, recombinant) can be used for the quantitative detection of ATP, glucose and creatine kinase.
For research use only. We do not sell to patients.
- CAS No.: 9001-40-5
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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HY-125863
Glucose-6-phosphate dehydrogenase, Microorganism -
HY-125863C
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HY-P2902
Glucose oxidase -
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Glycerol-3-phosphate oxidase, E. coli -
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NAD-dependent Glucose dehydrogenase -
HY-135849
Catalase, Aspergillus niger -
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α-Glucosidase, bacillus stearothermophilus -
HY-P2755
Xanthine oxidase, Microorganism -
HY-P2755A
Xanthine oxidase, bovine milk -
HY-135849A
Catalase, Bovine Liver
Biological Activity
Description
In Vitro
Glucose 6-phosphate dehydrogenase (yeast, recombinant) (G6PD (yeast, recombinant)) (1 mM, 2 mM, 0.2 mM; 20 h, 20 min, 25 °C) is a tetrameric, strictly NADP+-dependent enzyme with an optimal pH of 8.5, optimal temperature of 47.5 °C, a Km for NADP+ of 33.3 µM, and is strongly inhibited by Zn2+ and NADPH (Ki = 56.04 µM)[1].
Glucose 6-phosphate dehydrogenase (yeast, recombinant) (1 mM, 2 mM, 0.2 mM, 0.2 mg/mL; 20 h, 25 °C) has Arg-52 as an essential determinant for NADP+ binding, as the R52D mutant loses all NADP+-dependent activity; only the quadruple R52D/S53Q/Y93V/A124S mutant gains weak NAD+-dependent activity with a Km of 3929 µM, with no change to enzyme structure or oligomerization[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9001-40-5
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Appearance Solid
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Color White to off-white
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SMILES
[Glucose 6-phosphate dehydrogenase (yeast, recombinant)]
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Synonyms
G6PD (yeast, recombinant)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Glucose 6-phosphate dehydrogenase (yeast, recombinant) Related Classifications
HY-125863B Related Classifications
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