IRG1-IN-1

Name: IRG1-IN-1
Brand: MedChemExpress
SKU: HY-148335
Rating: 5.0 (9 reviews)

Cat. No.: HY-148335 Purity: 99.80%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

IRG1-IN-1 is an itaconic acid derivative. IRG1-IN-1 can inhibit immune-responsive gene 1 (IRG1) activity. IRG1-IN-1 can be used for the research of cancer, inflammation and autoimmune diseases.

For research use only. We do not sell to patients.

CAS No. : 2407652-42-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

9 Publications Citing Use of MCE IRG1-IN-1

In Vivo Efficacy Study

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

: IRG1-IN-1 purchased from MedChemExpress. Usage Cited in: Nature. 2026 Apr;652(8111):1004-1015.; Fold change in tumour volume of mice with ZFTA–RELA mNSC xenografts and treated with vehicle (control; n = 5), an ACOD1-specific inhibitor (IRG1-IN-1, 1 mg/kg, intraperitoneally (i.p.); n = 6) or a combination of IRG1-IN-1 (1 mg/kg, i.p.) and itaconate (25 mg/kg, i.v.; n = 8). IRG1-IN-1 significantly slowed tumour growth.

: IRG1-IN-1 purchased from MedChemExpress. Usage Cited in: Nature. 2026 Apr;652(8111):1004-1015.; Western blot analysis of ZFTA–RELA, endogenous RELA and GAPDH levels was performed in tumour tissues (n = 3 per group) from mice bearing ZFTA–RELA mNSC xenografts. Mice were treated with vehicle, IRG1-IN-1 (1 mg/kg; i.p.; once every other day for 2 weeks), or the combination of IRG1-IN-1 and itaconate (25 mg/kg; i.v.). Tumour images are shown below. IRG1-IN-1 reduced ZFTA–RELA protein levels in vivo, and this effect was rescued by itaconate supplementation.

: IRG1-IN-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 26;16(1):10551. [Abstract]; RAW264.7 cells treated with IRG1-IN-1 (1 mM) and Su3118 were infected with S. Typhi encoding the nanoluciferase-dependent itaconate biosensor for 22 h.

: IRG1-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2025 Sep 22:e70130.; Western blots were used to verify the protein expression level of LCN2 and ACOD1 after overexpression of LCN2 with or without 20 μM IRG1-IN-1 (ACOD1-IN) after 48 h of treatment. IRG1-IN-1 (ACOD1-IN) inhibited the upregulation of ACOD1 protein expression levels.

: IRG1-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2025 Sep 22:e70130.; Cell viability of C2C12 myoblasts after overexpression of LCN2 either alone or in the presence of 20 μM IRG1-IN-1 (ACOD1-IN) after 48 h of treatment. ACOD1-IN alleviated the inhibitory effect of LCN2 on cell viability.

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Description

IRG1-IN-1 is an itaconic acid derivative. IRG1-IN-1 can inhibit immune-responsive gene 1 (IRG1) activity. IRG1-IN-1 can be used for the research of cancer, inflammation and autoimmune diseases^[1].

In Vitro

IRG1-IN-1(compound 6) (0.5 mM; 2 mM) reduces production of itaconic acid and the secretion of TNFα from LPS-stimulated human monocyte derived macrophages (hMDMs) ^[1].
IRG1-IN-1 (0.5 mM; 1 mM) inhibits the proliferation of C-IRG1-9 rat glioma cells^[1].
IRG1-IN-1(10 nM) increases proliferation of TCR-activated hCD8⁺ T cells^[1].
IRG1-IN-1(10μM) shows depletion of trimethylation of histone 3 at lysine 4 (H3K4me3) in CR-activated hCD8⁺ T cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	TCR-activated hCD8⁺ T cells
Concentration:	10 μM
Incubation Time:	24-72 h
Result:	Decreased protein levels of histone 3 (H3).

Cell Proliferation Assay^[1]

Cell Line:	C-IRG1-9 rat glioma cells and TCR-activated hCD8⁺ T cells
Concentration:	0.5 mM; 1 mM; 10 nM
Incubation Time:	48-96 h
Result:	Inhibited the proliferation of C-IRG1-9 rat glioma cells and increased proliferation of TCR-activated hCD8⁺ T cells.

In Vivo

IRG1-IN-1 (compound 6) (i.p.; 0.2 mg/kg; 27 days) shows antitumor effect in C57BL/6 mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice^[1]
Dosage:	0.2 mg/kg
Administration:	IP; 27 days
Result:	Increased survival of C57BL/6 mice bearing mouse CT26 colorectal tumors. Decreased intratumoral frequency of M-MDSCs in tumors.

Molecular Weight

314.31

Formula

C₁₈H₁₅FO₄

CAS No.

2407652-42-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)/C(C(CC1=CC=CC=C1)C(O)=O)=C\C2=CC=C(F)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (318.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1816 mL	15.9079 mL	31.8157 mL
5 mM	0.6363 mL	3.1816 mL	6.3631 mL
10 mM	0.3182 mL	1.5908 mL	3.1816 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (253 KB) HNMR (254 KB) RP-HPLC (261 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Adonia Papathanassiu, et al. Compositions and methods of using itaconic acid derivatives. Patent. US20210261495A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1816 mL	15.9079 mL	31.8157 mL	79.5393 mL
	5 mM	0.6363 mL	3.1816 mL	6.3631 mL	15.9079 mL
	10 mM	0.3182 mL	1.5908 mL	3.1816 mL	7.9539 mL
	15 mM	0.2121 mL	1.0605 mL	2.1210 mL	5.3026 mL
	20 mM	0.1591 mL	0.7954 mL	1.5908 mL	3.9770 mL
	25 mM	0.1273 mL	0.6363 mL	1.2726 mL	3.1816 mL
	30 mM	0.1061 mL	0.5303 mL	1.0605 mL	2.6513 mL
	40 mM	0.0795 mL	0.3977 mL	0.7954 mL	1.9885 mL
	50 mM	0.0636 mL	0.3182 mL	0.6363 mL	1.5908 mL
	60 mM	0.0530 mL	0.2651 mL	0.5303 mL	1.3257 mL
	80 mM	0.0398 mL	0.1988 mL	0.3977 mL	0.9942 mL
	100 mM	0.0318 mL	0.1591 mL	0.3182 mL	0.7954 mL

IRG1-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IRG1-IN-12407652-42-8Drug DerivativeDrug derivativeitaconic acidderivativeimmune-responsive gene 1 (IRG1)cancerinflammationautoimmune diseasesInhibitorinhibitorinhibit

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IRG1-IN-1

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IRG1-IN-1 Related Antibodies

9 Publications Citing Use of MCE IRG1-IN-1

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•Related Screening Libraries:

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•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

IRG1-IN-1 Related Antibodies

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Complete Stock Solution Preparation Table

IRG1-IN-1 Related Classifications

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