Cat. No.: HY-N0820

Catalpol

(Synonyms: Catalpinoside)

13 Cited Publications

Customer Review

Based on 13 publication(s) in Google Scholar

Catalpol (Catalpinoside), an iridoid glycoside found in Rehmannia glutinosa. Catalpol has neuroprotective, hypoglycemic, anti-inflammatory, anti-cancer, anti-spasmodic, anti-oxidant effects and anti-HBV effects.

For research use only. We do not sell to patients.

Purity: 99.86%
CAS No.: 2415-24-9
Formula: C₁₅H₂₂O₁₀
Molecular Weight:362.33

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Catalpol Chemical Structure

CAS No.: 2415-24-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (13)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	GI₅₀	>100 μM Compound: 1, catalpol	Antiproliferative activity against human A2780 cells after 48 hrs by SRB assay Antiproliferative activity against human A2780 cells after 48 hrs by SRB assay	[PMID: 17178223]
A2780	GI₅₀	>100 μM Compound: 2	Antiproliferative activity against human A2780 cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human A2780 cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
A2780	IC₅₀	>10 μM Compound: 10	Cytotoxicity against human A2780 cells by MTT assay Cytotoxicity against human A2780 cells by MTT assay	[PMID: 26859776]
A549	IC₅₀	>10 μM Compound: 10	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 26859776]
Bel-7402	IC₅₀	>10 μM Compound: 10	Cytotoxicity against human Bel7402 cells by MTT assay Cytotoxicity against human Bel7402 cells by MTT assay	[PMID: 26859776]
BGC-823	IC₅₀	>10 μM Compound: 10	Cytotoxicity against human BGC823 cells by MTT assay Cytotoxicity against human BGC823 cells by MTT assay	[PMID: 26859776]
BV-2	IC₅₀	7.3 μM Compound: 10	Inhibition of LPS-induced NO production in mouse BV2 cells assessed as reduction in nitrite level pretreated for 24 hrs before LPS treatment for additional 24 hrs by Griess reaction method Inhibition of LPS-induced NO production in mouse BV2 cells assessed as reduction in nitrite level pretreated for 24 hrs before LPS treatment for additional 24 hrs by Griess reaction method	[PMID: 26859776]
HBL-100	GI₅₀	>100 μM Compound: 2	Antiproliferative activity against human HBL100 cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human HBL100 cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
HeLa	GI₅₀	>100 μM Compound: 2	Antiproliferative activity against human HeLa cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human HeLa cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
HT-29	IC₅₀	>10 μM Compound: 10	Cytotoxicity against human HT-29 cells by MTT assay Cytotoxicity against human HT-29 cells by MTT assay	[PMID: 26859776]
SW1573	GI₅₀	>100 μM Compound: 1, catalpol	Antiproliferative activity against human SW1573 cells after 48 hrs by SRB assay Antiproliferative activity against human SW1573 cells after 48 hrs by SRB assay	[PMID: 17178223]
SW1573	GI₅₀	>100 μM Compound: 2	Antiproliferative activity against human SW1573 cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human SW1573 cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
T47D	GI₅₀	>100 μM Compound: 1, catalpol	Antiproliferative activity against human T47D cells after 48 hrs by SRB assay Antiproliferative activity against human T47D cells after 48 hrs by SRB assay	[PMID: 17178223]
T47D	GI₅₀	>100 μM Compound: 2	Antiproliferative activity against human T47D cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human T47D cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
WiDr	GI₅₀	>100 μM Compound: 1, catalpol	Antiproliferative activity against human WiDr cells after 48 hrs by SRB assay Antiproliferative activity against human WiDr cells after 48 hrs by SRB assay	[PMID: 17178223]
WiDr	GI₅₀	>100 μM Compound: 2	Antiproliferative activity against human WiDr cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human WiDr cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]

In Vitro

Catalpol (0.1 μg/mL; for 3 days) can induce neuronal differentiation in PC12 cells through activation of the intracellular signal transduction pathway, and promote neurite length^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Catalpol (25-100 mg/kg; intraperitoneal injection; once) treatment clearly reduces blood urea nitrogen, serum creatinine levels and the expression of KIM-1 in renal ischemia/reperfusion-injury (IRI) mice. Catalpol markedly reduces the expression of PI3K, Akt and eNOS levels, and suppresses the TNF-α, IL-1β, IL-6 and IL-10 activities^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 mice treated with renal ischemia/reperfusion surgery^[1]
Dosage:

25 mg/kg, 50 mg/kg and 100 mg/kg
Administration:

Intraperitoneal injection; once
Result:

Clearly reduced blood urea nitrogen, serum creatinine levels and the expression of KIM-1 in renal IRI mice.

Chemical Information

CAS No. 2415-24-9
Appearance Solid
Molecular Weight 362.33
Formula C₁₅H₂₂O₁₀
Color White to off-white
SMILES

OC[C@@]12[C@]([C@@H]3O[C@]([C@@H]([C@@H](O)[C@@H]4O)O)([H])O[C@@H]4CO)([H])[C@](C=CO3)([H])[C@H](O)[C@@H]1O2
Synonyms

Catalpinoside
Structure Classification
- Terpenoids
- Iridoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (13)

All IFWBBio/Physico-chemical Assay

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Journal Impact Factor

Pharmacol Res

Cardamonin retards progression of autosomal dominant polycystic kidney disease via inhibiting renal cyst growth and interstitial fibrosis. [Abstract]

2020 May;155:104751. PMID: 32151678
Int J Biol Macromol

Reticuline isomerase AKR1B1 with aldo-keto reductase activity and detoxification function from the insect Blaps rhynchopetera. [Abstract]

2026 Mar:352:151224. PMID: 41791543
Acta Pharmacol Sin

Catalpol improves impaired neurovascular unit in ischemic stroke rats via enhancing VEGF-PI3K/AKT and VEGF-MEK1/2/ERK1/2 signaling. [Abstract]

2022 Jul;43(7):1670-1685. PMID: 34795412

Catalpol purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2022 Jul;43(7):1670-1685. [Abstract]

The numbers of vessels and neurons in the cortex near the infarcted area were up-regulated in Catalpol (Rat; i.v, 2.5, 5.0, 10.0 mg/kg/d; for 14 d) groups.
Phytomedicine

Catalpol protects vascular structure and promotes angiogenesis in cerebral ischemic rats by targeting HIF-1α/VEGF. [Abstract]

2020 Nov;78:153300. PMID: 32866905
Bioeng Transl Med

Catalpol promotes the generation of cerebral organoids with oRGs through activation of STAT3 signaling. [Abstract]

2024 Dec 29;10(3):e10746. PMID: 40385540

Catalpol purchased from MedChemExpress. Usage Cited in: Bioeng Transl Med. 2024 Dec 29;10(3):e10746. [Abstract]

Organoids (generation of cerebral organoids; 22 D; 10 uM). Immunofluorescence staining for TBR1 (red), SOX2 (green), and DAPI (blue) in day 30 control and Catalpol-treated organoids.
J Ethnopharmacol

Protecting the brain from stroke: Huoxue Rongluo formula (HXRLF) targets ferroptosis for neuroprotection. [Abstract]

2025 Jul 29;353(Pt A):120329. PMID: 40744419
J Ethnopharmacol

Radix Rehmanniae Praeparata promoted zebrafish fin regeneration through aryl hydrocarbon receptor-dependent autophagy. [Abstract]

2024 May 6:331:118272. PMID: 38710459

Catalpol purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 May 6:331:118272. [Abstract]

Bar charts showing the length of the regenerating fin of zebrafish exposure to different concentrations of Catalpol (2 days) at 2 dpa, n = 8~20. The results indicated that 0.5 mg/L was the optimal concentration for Catalpol to exert its pro-regenerative effect.
Sci Rep

Catalpol regulates apoptosis and proliferation of endothelial cell via activating HIF-1α/VEGF signaling pathway. [Abstract]

2024 Nov 16;14(1):28327. PMID: 39550364

Catalpol purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Nov 16;14(1):28327. [Abstract]

Catalpol (HUVECs; 1, 10, 100 µM; for 24 h). Western blots were conducted to detect the Bax, Bcl2, Cyto C, C-casp3 expression level. β-Actin was used as the endogenous control.
Chem Biol Drug Des

Promotion Effect of Catalpol on Angiogenesis and Potential Mechanisms: A Research Based on Network Pharmacology. [Abstract]

2024 Aug;104(2):e14602. PMID: 39134897
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Microvasc Res

Catalpol alleviates myocardial ischemia reperfusion injury by activating the Nrf2/HO-1 signaling pathway. [Abstract]

2022 Mar:140:104302. PMID: 34919942
SSRN

2021 Oct.
Research Square Preprint

2021 Feb.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (275.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 7.14 mg/mL (19.71 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7599 mL

13.7996 mL

27.5992 mL

5 mM

0.5520 mL

2.7599 mL

5.5198 mL

10 mM

0.2760 mL

1.3800 mL

2.7599 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jili Zhu, et al. Catalpol protects mice against renal ischemia/reperfusion injury via suppressing PI3K/Akt-eNOS signaling and inflammation. Int J Clin Exp Med. 2015 Feb 15;8(2):2038-44. [Content Brief]

[2]. M Yamazaki, et al. Neuritogenic effect of natural iridoid compounds on PC12h cells and its possible relation to signaling protein kinases. Biol Pharm Bull. 1996 Jun;19(6):791-5. [Content Brief]

[3]. R Mehrotra, et al. In vitro studies on the effect of certain natural products against hepatitis B virus. Indian J Med Res. 1990 Apr;92:133-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.7599 mL	13.7996 mL	27.5992 mL	68.9979 mL
	5 mM	0.5520 mL	2.7599 mL	5.5198 mL	13.7996 mL
	10 mM	0.2760 mL	1.3800 mL	2.7599 mL	6.8998 mL
	15 mM	0.1840 mL	0.9200 mL	1.8399 mL	4.5999 mL
DMSO	20 mM	0.1380 mL	0.6900 mL	1.3800 mL	3.4499 mL
	25 mM	0.1104 mL	0.5520 mL	1.1040 mL	2.7599 mL
	30 mM	0.0920 mL	0.4600 mL	0.9200 mL	2.2999 mL
	40 mM	0.0690 mL	0.3450 mL	0.6900 mL	1.7249 mL
	50 mM	0.0552 mL	0.2760 mL	0.5520 mL	1.3800 mL
	60 mM	0.0460 mL	0.2300 mL	0.4600 mL	1.1500 mL
	80 mM	0.0345 mL	0.1725 mL	0.3450 mL	0.8625 mL
	100 mM	0.0276 mL	0.1380 mL	0.2760 mL	0.6900 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

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Catalpol

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Publications Citing Use of MedChemExpress (MCE) Catalpol

Biological Activity

Chemical Information

Publications (13)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Catalpol Related Classifications

HY-N0820 Related Classifications